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عملي مايكرو Flashcards

(188 cards)

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1
Q
A

( Flame source )

Dry heat is to kill microorganisms

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2
Q
A

( Safety box )

Contaminated sharp to be incinerated

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3
Q
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( Disposable bag )

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4
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Sodium hypochlorite ( CLORX )

Use : Decontamination of liquid waste , surface and some medical equipment.

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5
Q
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Iodophores

Use : 1- hand hygiene
2- it can applied on mucous membranes

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6
Q
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Gram negative rod , Gram stain

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7
Q
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Filamentous fungi

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8
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Chocolate agar “heated blood agar”

Can be cultured with : Haemophilus influenza Neisseria

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9
Q
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Blood agar

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10
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MacConkeys agar

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11
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Thiosulfate - citrate - bile salts - sucrose agar ( TCBS )

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12
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Robertson CMM ( Enriched )

For growth of anaerobic organism

Eg. Clostridium

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13
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A

( Metalic loop )

Method of sterilization is by Red heat

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14
Q

Identify :
Stain :
Shape :
Example of possible organisms :

A 
Identify : 
Spirochetes 
Stain :
Fontana stain 
Shape : 
Twisted spirally ( corkscrew ) 
Example of possible organisms : 
 Treponema Pallidum
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15
Q

Identify ?

Stain ?

Arrangement ?

Example ?

A

Identify : Staphylococci in Culture
Stain : Gram +ve
Arrangement : Grape-like irregular clusters
Example : S.aureus , S.epidermidis

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16
Q

Identify ?

Stain ?

Arrangement ?

Example ?

A

Identify : Streptococci, in Culture
Stain : Gram +ve
Arrangement : Chain
Example : S. pyogens , S. agalactiae & S. viridans

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17
Q

Identify ?
Stain ?
Arrangement ?

A

Identify : Corynebacterium Diphtheria
Stain : Gram + ve
Arrangement : V shape giving Chinese letter appearance

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18
Q 
Identify ? 
Stain ? 
shape ? 
Arrangement ? 
Example?
A 
Identify : Bacillus group 
Stain : Gram +ve ( gram stain ) 
shape : Rods 
Arrangement : Long Chains 
Example : B. anthracis & B. cereus
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19
Q
A

Identify : Bacillus group
Stain : Gram +ve ( spore stain )
shape : Rods
Arrangement : Long Chains

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20
Q
A

Clostridium tetani

G +ve bacilli arranged in Drum stick appearance with unstained terminal spores

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21
Q
A 
Identify : Gram Negative Diplococci
Stain : Gram –ve 
Shape : Diplococci 
Arrangement : Pairs 
Example : Neisseria meningitides & Neisseria gonorrhea
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22
Q
A

Identify: Gram –ve bacilli
Stain : Gram –ve
Shape : straight bacilli
Example : E. coli

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23
Q
A

Identify : Gram –ve bacilli
Stain : Gram –ve
Shape : curved bacilli
Example : Vibrio cholera , Helicobacter, Cambylobacter

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24
Q
A

Staphylococcus aureus in pus
Gram +ve cocci arranged in clusters ( Grape like
Staphylococci aureus
+ Present in pus

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25
Staphylococcus aureus in pus Gram +ve cocci arranged in clusters ( Grape like Staphylococci aureus + Present in pus
26
Staphylococcus aureus in pus Gram +ve cocci arranged in clusters ( Grape like Staphylococci aureus + Present in pus
27
Staphylococcus aureus in pus Gram +ve cocci arranged in clusters ( Grape like Staphylococci aureus + Present in pus
28
Streptococcus pyogenes in pus | Gram +ve cocci arranged in chains + present in pus
29
Streptococcus pyogenes in pus | Gram +ve cocci arranged in chains + present in pus
30
Streptococcus pyogenes in pus | Gram +ve cocci arranged in chains + present in pus
31
(/Meningococci / Gonococci ) | Gram -ve diplococci arranged in pairs with kidney shape appearance + present in pus
32
Bacillus anthracis in tissue | G +ve bacilli arranged in long chain + present in pus
33
Identify: Acid Fast Bacilli Stain : Ziehl Neelsen Example : Mycobacterium Tuberculosis in sputum
34
Mycobacterium tuberculosis in sputum stained by Ziehl Neelsen
35
Mycobacterium leprae | Stain : Modified Ziehl Neelsen
36
Id entify : Spirochetes Stain : Fontana stain Shape : Twisted spirally ( corkscrew ) Example : Treponema Pallidum
37
Gram +ve budding yeast Example ; Candida albicans
38
Specific test to differentiate between Candida albicans from other albicans?
Germ tube test
39
Gram +ve ( Candida albicans )
40
Cryptococcus neoformans | Black background by India ink stain
41
Cryptococcus neoformans | black background by Direct Immunofluoresce
42
CPE of CMV (Intranuclear inclusion bodies with Owl’s eye appearance)
43
CPE of CMV (Intranuclear inclusion bodies with Owl’s eye appearance)
44
Heavy duty gloves Safe for holding and cleaning of sharp instruments ( surgical scissors , and instruments )
45
Goggles Should be used to avoid splashes of infectious material to the eye
46
( Autoclave )
47
( swab stick ) | For collection of specimen
48
Nutrient agar (Basic culture media)
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( Endopigment )
50
Exopigment
51
( Macconkeys agar media ) Showing lactose fermenting colonies ( LFC ) Example : E.coli
52
Lactose fermenter on MacConkey agar | Example : klebsiella
53
Blood agar Beta hemolytic Example : S.aureus
54
Blood agar | Alpha hemolysis
55
Filamentous fungi , Aspergillus in culture, lactophenol cotton blue stain .
56
Heavy duty gloves Safe for holding and cleaning of sharp instruments ( surgical scissors , and instruments )
57
Goggles Should be used to avoid splashes of infectious material to the eye
58
Surgical mask To prevent transmission of droplets and protect against infection.
59
Lowenstein – Jensen Medium Uses: For isolation of mycobacterium tuberculosis
60
Loffler's Serum Media Type: Enriched Uses: isola:on of Corynebacterium diphtheria
61
Robertson's Cooked Meat Media Type: Enrichment Uses: For growth of anaerobic organism e.g. Clostridium
62
البول الاسود، جوا
Bile Aesculin Agar showing ( Black discoloration ) | Ex. Enterococcus
63
لاهوائي و غاز و ظهر الجار
Anaerobic Gas Pack Jar Uses: - For isolation of anaerobic organisms e.g. all Clostridium sp. For isolation of microaerophilic microorganisms e.g. Campylobacter & Helicobacter
64
انا بروفيسوره هندية
AP I (Analytical Profile Index) test
65
الازرق كوب عليه مويه KP | الاخضر يعني إفا E
Citrate utilization test Positive (Blue) Ex: 1- Klebsiella 2- pseudomonas aeroginosa Negative (Green) Ex. E.coli
66
المجنون يحب اللون الوردي اكثر من مره و يحب الجلوكوز و المالتوز
Sugar utilization test for Neisseria Meningococci: Showing fermentation of glucose and maltose
67
جنى تحب اللون الاصفر و الوردي و تحب الجلوكوز بس
Gonococci: Showing fermentation of glucose ONLY
68
Brucella slide test , slide agglutination test | For serotyping of bacteria
69
Swarming of proteus on nutrient agar
70
ثايو السافل ستر البول المالح السكروزي
Thiosulphate citrate bile salt sucrose agar (TCBS) Growth of non sucrose fermenting colonies Use in : 1- vibrio genus 2- vibro cholera
71
MacConkey agar Growth of lactose fermenter ( LFC ) Use in : E. Coli
72
MacConkey agar Growth of lactose fermenter ( LFC ) Use in : E. Coli
73
MacConkey agar Growth of lactose fermenter ( LFC ) Use in : E. Coli
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MacConkey agar Non lactose fermenter | Proteus
75
Custine lactose Electrolyte Deficient agar ( CLED ) Growth of lactose fermenter Semiquantitave urine culture E. Coli
76
Custine lactose Electrolyte Deficient agar ( CLED ) Growth of lactose fermenter Semiquantitave urine culture E. Coli
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Exopigment of pseudomonas on nutrient agar
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Exopigment of pseudomonas on nutrient agar
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Exopigment of pseudomonas on Mac Conkey agar
80
``` Staphylococcus aureus in pus Gram +ve cocci arranged in clusters ( Grape like Staphylococci aureus + Present in pus Virulence factor: 1- Protein A 2- Clumping factors (bound coagulase) Disease : Impetigo ```
81
``` Staphylococcus aureus in pus Gram +ve cocci arranged in clusters ( Grape like Staphylococci aureus + Present in pus Virulence factor: 1- Protein A 2- Clumping factors (bound coagulase) Disease : Impetigo ```
82
``` Staphylococcus aureus in pus Gram +ve cocci arranged in clusters ( Grape like Staphylococci aureus + Present in pus Virulence factor: 1- Protein A 2- Clumping factors (bound coagulase) Disease : Impetigo ```
83
``` Staphylococcus aureus in pus Gram +ve cocci arranged in clusters ( Grape like Staphylococci aureus + Present in pus Virulence factor: 1- Protein A 2- Clumping factors (bound coagulase) Disease : Impetigo ```
84
``` Streptococcus pyogenes in pus Gram +ve cocci arranged in chains + present in pus Virulence factor: 1- M protein 2- IgG degrading enzyme Disease : 1- acute pharyngitis 2-impetigo Treatment: penicillin G ```
85
``` Streptococcus pyogenes in pus Gram +ve cocci arranged in chains + present in pus Virulence factor: 1- M protein 2- IgG degrading enzyme Disease : 1- acute pharyngitis 2-impetigo Treatment: penicillin G ```
86
Pneumococci Gram +ve cocci arranged in chains or diplos surrounded by unstained area + present in pus Virulence factors: 1- Polysaccharide capsule 2- IgA1 protease Diseases: 1- Otitis media 2- Lobar pneumonia
87
Pneumococci Gram +ve cocci arranged in chains or diplos surrounded by unstained area + present in pus Virulence factors: 1- Polysaccharide capsule 2- IgA1 protease Diseases: 1- Otitis media 2- Lobar pneumonia
88
Pneumococci Gram +ve cocci arranged in chains or diplos surrounded by unstained area + present in pus Virulence factors: 1- Polysaccharide capsule 2- IgA1 protease Diseases: 1- Otitis media 2- Lobar pneumonia
89
Pneumococci Gram +ve cocci arranged in chains or diplos surrounded by unstained area + present in pus Virulence factors: 1- Polysaccharide capsule 2- IgA1 protease Diseases: 1- Otitis media 2- Lobar pneumonia
90
Meningococci / Gonococci | Gram -ve diplococci arranged in pairs with kidney shape appearance + present in pus
91
Bacillus anthracis in tissue G +ve bacilli arranged in long chain + present in pus Virulence: 1-polypeptide Capsule 2- Anthrax toxin
92
Identify : Acid fast bacilli Stain : Ziehl Neelsen Example : Mycobacterium Tuberculosis in sputum
93
Mycobacterium leprae arranged in masses or groups mostly intracellular stained by Modified Ziehl Neelsen
94
Candida albicans | Germ tube test +ve
95
Candida albicans | Germ tube test -ve
96
Candida albicans | Germ tube test +ve
97
Cryptococcus neoformans, Capsulated budding yeast (India ink stain)
98
Cryptococcus neoformans, Capsulated budding yeast (India ink stain)
99
Cryptococcus neoformans, Capsulated budding yeast (India ink stain)
100
Cryptococcus neoformans Direct Immunofluoresce
101
Aspergillus flavus (lactophenol cotton blue)
102
CPE of Herpes simplex (Rounding and ballooning of cells grape like)
103
CPE of Herpes simplex (Rounding and ballooning of cells grape like)
104
CPE of RSV (Fusion of cells with syncytia formation) Syncytial formation caused by RSV in cell culture ) Disease :bronchiolitis and pneumonia in infants
105
Nutrient agar (Basic culture media)
106
Spicemen: ( CSF ) , blood , Nasopharyngeal swap in carrier’s ?
``` Chocolate agar (heated blood agar) (Enriched) Neisseria meningitis ```
107
Spicemen : urethral discharge, cervical samples , conjunctival swap ?
modified Thayer Martin medium | Neisseria gonorrhoea
108
Spicemen : sputum , CSF , pus ?
``` Chocolate agar (heated blood agar) (Enriched) Haemophilus influenza ```
109
Thiosulph ate citrate bile salt sucrose agar (TCBS) Uses: Isolation of vibrio Can be cultured with: Vibrio cholerae showing yellow sucrose fermenting colonies
110
Thiosulphate Citrate Bile Salt Sucrose Agar (TCBS) Cultured with: vibrio parahemolyticus Showing: colorless non lactose fermenting colonies
111
Cysteine Lactose Electrolyte deficient media (CLED) | Used for urine culture to inhibit swarming of proteus
112
Cysteine Lactose Electrolyte deficient media (CLED) | Non selective differential/indicator media for isolation of urinary pathogens
113
Loffler’s serum media Type: Enriched Uses: Rapid isola:on of Corynebacterium diphtheria
114
Lowenstein – Jensen Media Type: Enriched, Selective & Indicator (differential) Uses: For isolation of mycobacterium tuberculosis
115
Robertson's Cooked Meat Media | Uses: For growth of anaerobic organism e.g. Clostridium
116
Nutrient Agar Endopigment Staphylococcus aureus give golden yellow endopigment on nutrient agar
117
Nutrient agar Exopigment Pepseudomonas aeruginosa give greenish exopigment on nutrient agar
118
( Cell culture for virus culture )(For virus isolation and identification ) -Viral culturing: e.g. Polymyelitis & Herbs viruses
119
Anaerobic Gas Pack Jar Us es: - For isolation of anaerobic organisms e.g. all Clostridium sp. - For isolation of microaerophilic microorganisms e.g. Campylobacter & Helicobacter
120
Novobiocin sensitivity To Differentiates S. epidermidis from S. saprophyticus
121
حساسة ما تحب احد يجي جنبها
Novobiocin sensitivity S. epidermidis Skin Sensitive
122
ليست حساسة و الكل جنبها
( Novobiocin sensitivity ) S. saprophyticus URINE Resistance
123
Novobiocin sensitivity S. epidermidis Skin Sensitive
124
( Novobiocin sensitivity ) S. saprophyticus URINE Resistance
125
مكتوب على الدائرة B او A كلهم نفس الشيء
Bacitracin sensitivity | Group A streptococci sensitive ( streptococcus pyogenes)
126
مكتوب على الدائرة B او A كلهم نفس الشيء
Bacitracin resistant | Streptococcus group A , resistant ( streptococcus agalactiae )
127
Bacitracin sensitivity | Group A streptococci sensitive ( streptococcus pyogenes)
128
Bacitracin sensitivity | Group A streptococci sensitive ( streptococcus pyogenes)
129
Bacitracin sensitivity | Group A streptococci sensitive ( streptococcus pyogenes)
130
O او P نفس الشيء
Optochin sensitivity (Pneumococci sensitive)
131
O او P
Optochin sensitivity (Pneumococci sensitive)
132
O او P
Optochin sensitivity (Pneumococci sensitive)
133
``` Optochin sensitivity optochin resistance ( Viridans streptococci ) ```
134
است
Antibiotic Sensitivity Testing (Disc diffusion method) Used fo test antibiotics sensitivity by Disc diffusion method
135
ELEK’s test (Ag/Ab precipitation test, double diffusion of Ag/Ab in gel) Toxigenic strains of C.diphtheria
136
ELEK’s test | Toxigenic C.diphtheria
137
ELEK’s test | Non - toxigenic C.diphtheria
138
ELEK’s test
139
Nagler’s reaction (toxin neutralization) Clostridium perfringenes Antitoxin
140
Nagler’s reaction (toxin neutralization) Clostridium perfringenes No Antitoxin
141
Nagler’s reaction Used for toxin neutralization of clostridium perfringenes
142
Pseudomonas aeruginosa on nutrient agar | showing greenish exopigment
143
Proteus on nutrient agar showing swarming growth
144
Mannitol salt agar
145
Mannitol salt agar | Staphylococcus aureus
146
Mannitol salt agar | Staphylococcus epidermidis
147
Mannitol salt agar | Staphylococcus aureus
148
Mannitol salt agar
149
Mannitol salt agar | Staphylococcus aureus
150
الثقيلة
Lactose fermenting colonies in MacConkeys agar and mucoid is Klebsiella
151
الثقيلة
mucoid and Lactose fermenting colonies in MacConkeys agar is klebsiella
152
الخطوط الخفيفة
MacConkeys agar Lactose fermenting colonies , E. coli
153
MacConkeys agar Lactose fermenting colonies , E. coli
154
Non Lactose fermenting colonies (NLFC) •Salmonella •Shigella •Proteus
155
Non hemolytic colonies
156
Blood agar , Non hemolytic colonies
157
Blood agar , Non hemolytic colonies
158
Blood Agar à Swarming Growth | Cultured with: Proteus
159
Blood tellurite (Black colonies of Corynaebacterium diphtheria)
160
Blood tellurite (Black colonies of Corynaebacterium diphtheria)
161
Blood tellurite (Black colonies of Corynaebacterium diphtheria)
162
Alpha hemolysis in Chocolate agar (pneumococci/Strept virridans)
163
Sabouraud’s dextrose agar Filamentous fungi
164
Bcb
Blood cultu re bottles Type : Enrichment Used for culture of blood Examples/ culture of circulating organisms in blood In case of Bacteremia, septicaemia, typhoid fever
165
Tube agglutination test (standard agglutination test; SAT) e.g diagnosis of brucellosis
166
Tube agglutination test (standard agglutination test; SAT) e.g Prozone phenomenon
167
Tube agglutination test (standard agglutination test; SAT) e.g Prozone phenomenon
168
انتي الانبوبه الحمراءinti الدويري O
Antistreptolysin O test (ASOT) for diagnosis of rheumatic fever (+ve tube showing no hemolysis)
169
الدوائر الصفراء يعني ترتر صغير MT حق اليسا
Microtiter plate (ELISA plate) (Detection of Ag or Ab)
170
Coagulase production test , positive, staphylococcus aureus
171
Coagulase production test , negative, staphylococcus epidermidis
172
Urease production test Positive , proteus
173
Urease production test , negative
174
بيه
Bile esculin hydrolysis test (Positive  Enterococci)
175
Agglutination test Blood grouping
176
Slide agglutination test serogrouping/serotyping of bacteria
177
Catalase test , catalase positive, staphylococcus aureus
178
Catalase test , catalase positive , staphylococci
179
Catalase test , catalase negative , streptococci
180
Catalase test , catalase positive , staphylococci
181
Catalase test , catalase negative , streptococci
182
Oxidase test
183
Oxidase test , Oxidase positive , Neisseria , pseudomonas
184
Oxidase test , oxidase negative , streptococci
185
Oxidase test
186
Oxidase test
187
است
Antibiotic sensitivity Testing Used for test antibiotics sensitivity by Disc diffusion method
188
Identify: Antiseptics Uses : used for hand hygiene, preparation of patient skin before invasive procedures Example : alcohols, chlorhexidine and iodophores (Betadine)

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