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1

Type of cell death involving CASPASE enzyme

APOPTOSIS

2

Surgical connection between two structure

Anastomosis

3

Organs removed and dissected individually un the body (MOST WIDELY USED)

Technology of RUDOLF VIRCHOW

4

IN-SITU DISSECTION in part combined with EN BLOC TECHNIQUE

Technology of CARL VON ROKITANSKY

5

EN BLOC technique

Technology of ANTON GHON

6

EN MASSE technique

Technology of MAURICE LETULLE

7

Cold knife procedure:

Knife:
Tse:
Environment:

Knife: -40 to -60’C
Tse: -5 to -10’C
Environment: 0 to -10’C

8

Cryostat temp.

-18 to -20’C

9

Freeze drying temp

Quenching: -160’C

Sublimation: -40’C

10

Fixation temp

Sx’cal spx: RT
Histochem, EM: 0-4’C

11

Formol Calcium temp

4’C

12

Vacuum embedding temp

2-4’C above the melting pt of wax

13

Automated impregnation temp

3’C above the melting pt of wax

14

*MANUAL IMPREGNATION temp

2-5’C above the melting pt of wax (55’C to 60’C)

15

EMBEDDING

5-10’C above the melting pt of wax

16

Floatation water bath*

6-10’C BELOW THE MELTING OF WAX (45’C to 50’C)

17

Melting pt of wax:
Lab temp: 20-24’C

54-58’C (56’C)

18

Melting pt of wax:
Lab temp: 15-18’C

50-54’C

19

Water present in paraffin wax can be removed by:

Heating wax at 100-105’C

20

Gives the fastest result for paraffin wax impregnation

Vacuum embedding mtd

21

In double embedding, tse is first infiltrated w/ ____, then embedded w/ ____

Celloidin
Paraffin

22

Are used for INFILTRATION and EMBEDDING

CGP3

Celloidin
Gelatin

Paraffin
Paraffin subs
Plastic

23

Autotechnicon can perform: (4)

Fixation
Dehydration
Clearing
Infiltration

DIC -F

24

Sliding microtome

1789: Adams

25

Rocking (Cambridge) microtome

Paldwell Trefall (1881)

26

Rotary microtome

Minot (1885-1886)

27

Freezing microtome

Queckett (1848)

28

Simplest microtome

Rocking (Cambridge)

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Microtome incorporated in the Cryostat machine

Rotary (Minot)

30

Bevel angle

27’-32’

31

Clearance angle (knife to tse block)

0-15’ (5-10’)

32

Knife recommended for cutting paraffin-embedded sections on rotary microtome

Biconcave knife

33

Removal of nicks ( heel to toe, EDGE 1st)

Honing

34

Honing stones (3)

Belgium yellow
Arkansas
Fine carborundum

FAB

35

Removal of burrs (toe to heel, EDGE LAST)

Stropping

36

Used in stropping

Paddle strop (made of horse leather)

37

Causes of tse brittleness

Prolonged fixation
Dehydration
Clearing
Paraffin infiltration

38

Will make the tse opaque and difficult to cut due to presence of alcohol

INCOMPLETE CLEARING

39

Examples of mordants

K alum in Ehlich’s hematoxylin

Iron in Weigert’s hematoxylin

40

Sealing the margins of the coverslip

Ringing

41

Source of MONOCLONAL AB

Mice

42

SOURCE of POLYCLONAL AB for immunohistochem techniques

Rabbit 🐰 *

✔️ GOAT 🐐
✔️ PIG 🐖
✔️SHEEP 🐑
✔️ HORSE 🐎
✔️ GUINEA PIG 🐹

43

Color of AMINOETHYLCARBAZOLE (AEC)

Red

44

Color of DIAMINOBENZIDINE (DAB)

BROWN

45

Consists of cellular material which gas been transferred from 1 smear to another, usually DURING STAINING

Floater

46

Floaters may lead to _____

False ➕ if POSITIVE FLOATERS adhere to negative smear

47

Best type of fixative for EXFOLIATIVE CYTOLOGY

Equal parts of ETHANOL:ETHER

*highly flammable

48

Most commonly used fixative for EXFOLIATIVE CYTOLOGY

95% ETHYL ALCOHOL

49

Mtd of choice for EXFOLIATIVE CYTOLOGY

Papanicolau smear

* difference from Mod. PAP mtd: mod has no BISMARCK BROWN

50

Pale, pink cytoplasm with dark PYKNOTIC NUCLEUS
Under estrogen influence, TRUE ACIDOPHILIA is observed

Mature superficial cell

51

Medium sized POLYHEDRAL/ELONGATED cells with VACUOLATED CYTO

Intermediate cells

52

BOAT-SHAPED 🚣‍♀️
strong tendency to FOLD or CURL on edges

NAVICULAR (INTERMEDIATE)

53

Contains GLYCOGEN that pushes NUCLEUS ASIDE

PREGNANCY CELL (intermediate)

54

Resembles FRIED-EGGS with SUNNY SIDE UP
⬆️ in MENOPAUSAL WOMEN

PARABASAL CELLS

55

Groups of 3 or more cells found during menstruation

ENDOMETRIAL CELLS

56

May present a HONEYCOMB 🍯 APPEARANCE when viewed on end

ENDOCERVICAL GLANDULAR CELL

57

For samples of ENDOCERVICAL CANAL

ENDOCERVICAL BRUSH

58

For HYSTERECTOMY PXS

VAGINAL SCRAPE

59

*For Hormonal EVALUATION

*Lateral vaginal scrape

60

For localization of VAGINAL ADENOSIS

Four quadrant vaginal scrape

61

For detection of HERPETIC LESIONS OF CARCINOMA

VULVAR SCRAPE

62

Equipments for VAGINAL ASPIRATION

Glass pipette
Rubber bulb

63

Equipments for SWAB SMEAR

Ayre’s spatula

64

Equipments for ENDOCERVICAL or ENDOMETRIAL ASPIRATIONS

Laryngeal cannula attached to a 10-cc syringe

65

Nuclear stain (Counterstains)

Red: NSCH
BLUE: MTC

Red:
Neutral red
Safranin O
Carmine
Hematoxylin

Blue:
Methylene blue
Toluidine Blue
Celestine blue

66

Cytoplasmic stains (counterstain)

Red: PEE
Yellow: POR
Green: 2LG’s

Red:
Phloxine B
Eosin Y
Eosin B


Yellow:
Picric acid
Orange G
Rose Bengal*


Green:
Lt Green
LISSAMINE green