Flashcards in SPECIAL STAINS Deck (19):
1
Sudan black*
Lipids : blue-black
2
Sudan IV (SCHARLACH R)*
Lipids (TAG) = red
* MOST COMMONLY USED
3
Oil red o*
Fat: BRILLIANT RED
4
Osmic acid stain*
Fats : black
5
Nile blue sulfate mtd
Neutral fats: pinkish red/Cholestrin esters and cholestrin
Fatty acids: lt red
Cerebrosides: lt blue
Fatty acids and soap: Deep blue to violet
6
Toluidine blue - acetone mtd
Sulfatide: metachromatic red-brown or yellow
7
Birohydride -PAS MTD
Gangliosides - red
8
CHO STAIN
forms H bonds
Selective and specific for glycogen
BEST CARMINE
9
Cho stain: Stain of choice for glycogen
PAS with diastase ctrl
10
Protein stain:
Histones and protaminase - green
Alkaline fast- green mtd
11
Special stain for Nucleic acid
MOST RELIABLE and SPECIFIC STAIN FOR DNA
Fuelgen technique
12
Special stain for Nucleic acid
RNA (nucleoli) = red
DNA (chromatin) = green
Methyl green - pyronin
13
Special stain for Nucleic acid
DNA: yellow-green fluorescence
RNA: brick to orange red fluorescence
Acridine orange fluorescent stain
14
Tse pigment & Deposits stain
Melanin and argentaffin cell granules - black
Masson-Fontana technique
15
Tse pigment & Deposits stain
Calcium- black
Von kossa’s silver nitrate mtd
16
Microorganism stain
M.leprae, other mycobacteria & nocardia
Wade-Fite technique
17
Microorganism stain
Spirochetes- black on yellow bg
Levaditi’s mtd
18
Microorganism stain
Spirochetes- black
Warthin-Starry mtd
19