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Flashcards in SPECIAL STAINS Deck (19):
1

Sudan black*

Lipids : blue-black

2

Sudan IV (SCHARLACH R)*

Lipids (TAG) = red
* MOST COMMONLY USED

3

Oil red o*

Fat: BRILLIANT RED

4

Osmic acid stain*

Fats : black

5

Nile blue sulfate mtd

Neutral fats: pinkish red/Cholestrin esters and cholestrin

Fatty acids: lt red

Cerebrosides: lt blue

Fatty acids and soap: Deep blue to violet

6

Toluidine blue - acetone mtd

Sulfatide: metachromatic red-brown or yellow

7

Birohydride -PAS MTD

Gangliosides - red

8

CHO STAIN
forms H bonds
Selective and specific for glycogen

BEST CARMINE

9

Cho stain: Stain of choice for glycogen

PAS with diastase ctrl

10

Protein stain:
Histones and protaminase - green

Alkaline fast- green mtd

11

Special stain for Nucleic acid
MOST RELIABLE and SPECIFIC STAIN FOR DNA

Fuelgen technique

12

Special stain for Nucleic acid

RNA (nucleoli) = red
DNA (chromatin) = green

Methyl green - pyronin

13

Special stain for Nucleic acid

DNA: yellow-green fluorescence
RNA: brick to orange red fluorescence

Acridine orange fluorescent stain

14

Tse pigment & Deposits stain

Melanin and argentaffin cell granules - black

Masson-Fontana technique

15

Tse pigment & Deposits stain
Calcium- black

Von kossa’s silver nitrate mtd

16

Microorganism stain

M.leprae, other mycobacteria & nocardia

Wade-Fite technique

17

Microorganism stain

Spirochetes- black on yellow bg

Levaditi’s mtd

18

Microorganism stain

Spirochetes- black

Warthin-Starry mtd

19

Microorganism stain

HBsAg, some mucins -brown black

Orcein mtd