1. class Flashcards by Thomas Hasiweder

Q

Why fluorescence microscopy?

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Q

Why fluorescence microscopy?

GFP & quantum dots

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Q

Basics of fluorescence

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Q

Nature of light

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Q

Visible light

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Q

Light absorption

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Q

Light absorption

text

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Q

Lambert-Beer Law

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Q

Absorption and emission spectra

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Q

Simplified Jablonski diagram

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Q

Asorption vs Fluorescence

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Q

Stokes shift

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Q

Jablonski diagram

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Q

Photophysical processes

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Q

Many things can happen…

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Q

Symmetry

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Q

Exceptions to the Mirror-Image Rule

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Q

Absorption and emission spectra

Quinine

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Q

Some history

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Q

Fluorescence quantum yield

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Q

Fluorescence lifetime

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Q

Fluorescence lifetime graph

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Q

Fluorescence lifetime

Eosin

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Q

Fluorescence lifetime

donor & acceptor

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Intrinsic chromophores

Extrinsic chromophores

Extrinsic chromophores labeling

Light-matter interactions absorption

Luminescence incandescence

Light-matter interactions reflection & refraction

QUESTION: How do a perfectly diffuse or specular reflective surface look like?

Measurement of absorption spectra

Measurement of fluorescence

Measurement of fluorescence spectra

Measurement of fluorescence quantum yield

Measurement of fluorescence quantum yield effects

Measurement of lifetime

Measurement of lifetime TAC reset:

Measurement of lifetime IRF

Example: CFP fluorescence decay

Example: CFP fluorescence decay heterogeneity

FLUORESCENCE ANISOTROPY

Polarized light and photoselection

photoselection

Anisotropy experiment

Sequence of events after excitation with polarized light

Example: YFP

Example: multichromophoric dendrimers

Example: multichromophoric dendrimers monomer vs dendrimer

Magic angle

Energy transfer

Radiative energy transfer

Energy transfer

Förster Resonance Energy Transfer: FRET

FRET

FRET R0

FRET ex & em

FRET The critical transfer distance

FRET efficiency

Some useful FRET numbers

Förster Resonance Energy Transfer: FRET

FRET can be used to study:

Dexter energy transfer

Electron transfer

Electron transfer photosynthesis

Using Chlorophyl Fluorescence to Study Photosynthesis