10. Class: labelfree Flashcards by Thomas Hasiweder

Q

Optical microscopy in biology?

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Q

content

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Molecular spectroscopy: matter

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Molecular spectroscopy: EM radiation

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Molecular spectroscopy: matter

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Light absorption and fluorescence

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Light absorption and fluorescence

transitions

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Resolution: diffraction limited

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Intrinsic fluorescence

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Fluorescence labeling

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Molecular spectroscopy: photon picture

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Vibrational energy

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(Spontaneous) Raman spectroscopy

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Raman vs Infrared spectrum

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Raman vs Infrared spectrum

wavelengths

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Raman vs Infrared spectrum

spectrum absorption

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Raman scattering intensity and shift

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Molecular spectroscopy: Light scattering

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Q

a new type of secondary radiation

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Q

Inelastic light scattering: Phenomenological

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Infrared spectroscopy / microscopy

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dimensions of biomolecules

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Q

Raman micro-spectroscopy

Study These Flashcards

A

Q

Raman microspectroscopy

advantages & disadvantages

Study These Flashcards

A

Raman µscopy: fixed lymphocytes

Conclusion on Raman spectroscopy and comparison with infrared

Stokes and anti-Stokes Raman scattering

SERS: phenomenological

Light induced Surface Plasmons

Electric field enhancement: Antenna-effect

Chemical enhancement

SERS in cells

Tip Enhanced RS (TERS)

TERS for cell membrane analysis

nonlinear?

Two-Photon excitation

Two-photon absorption

Two-Photon excitation

Two-Photon excitation =\> multiphoton

Auto-fluorescence

Pulsed lasers: time-averaged vs peak power!

2nd harmonic generation

2nd harmonic generation: what can we image?

Drosophila larvae

Nonlinear light-matter interactions

Coherent Raman Imaging: CARS

Coherent Raman Imaging: CARS

Coherent Raman Imaging: CARS Setup for microscopy?

Nonlinear optical microscope

Coherent Raman imaging

Coherent Raman Imaging: CARS

Drosophila polytene

Small molecule labeling

Small molecule labeling

end