1000 Histo Connective Tissue Flashcards by Danielle Hayes

What are the five types of connective tissue?

proper, bone, cartilage, blood, lymph

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How can proper connective tissue be further subcategorized?

loose, dense, adipose

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What are the 5 functions of connective tissues?

Mechanical support and protection of soft tissues
Physiological support
Storage of energy in fat
Defense against infection
repair of injuries

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How does CT give physiological support?

a. Serves as pathway for vessels and nerves
b. Tissue fluids of connective tissue act as
diffusion medium for exchange of metabolites between tissues and vessels

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If the connective tissue is damaged and unable to facilitate diffusion of fluids what results?

edema (fluid build up in tissues)

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What is the composition of connective tissue?

ground substance and fibers (ECM) and cells

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What kind of connective tissue has these:

           Fibrocytes      
           Fibroblasts
           Adipose Cells
           Mast Cells 
           Macrophages 
           Pericytes  
          (Chondrocytes)
          (Osteocytes)

Fixed (resident) CT

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What kind of connective tissue has these:
Plasma cells
   Lymphocytes
   Neutrophils
   Eosinophils
   Basophils
   Monocytes
   Macrophages

Free (transient) CT

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What is the composition of ground substance?

amorphous gel-like material composed of
GAGs
proteoglycans
glycoproteins

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What are GAGs?

Long, inflexible, polysaccharides composed of repeating disaccharide units

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If you link a repeating disaccharide unit (GAG) to a protein what do you get?

proteoglycan

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Glycoproteins are primarly (blank) molecules of the ECM. What are some examples of these.

cell adhesion
integrins, fibronectins, laminin, entactin,
tenascin, chondronectin and osteonectin

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(blank) is the most important nonsulfated GAG.
What is it important for?
Where is it found

hyaluronan
absorb semisolids (jello-like)
Found in the ECM of all CT and in vitreous humor and synovial fluid.

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The majority of GAGs are (blank)

sulfated

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Gags have a strong (blank) charge and attracts active (blank) causing a heavily hydrated matrix strongly resistant to compression.

negative

cations

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Since GAGS are negatively charged they repel each other and create a (blank)

slippery surface

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Some Important GAGS:
(blank) are well-represented in catrilage,
keratans and dermatins in (blank)
(blank) is crucial to the functioning of basal laminae, especially in the glomerular filtration membrane of the kidney.

chondriotins
skin
heparan

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What consists of a protein core to which sulfated GAGs are covalently bound (shaped like test tube brush). Form thick colloids similiar to gravy, pudding, gelatin or glue.

Proteoglycans

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What do proteoglycans do?

trap water, occupy space, link to collagen fibers, cell adhesion, slows the spread of pathogens through tissues.

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Why is it important the proteoglycans trap water?

resist compression

- return to original shape

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Why is it important the proteoglycans link to collagen fibers?

What do proteoglycans aid with via cell adhesion?

-to form networks
- in bone combine with calcium hydroxyapatite
and calcium carbonate

Embryonic migration

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What are glycoproteins?

cell adhesion molecules of the ECM, fibronection found in ECM, Laminin found in basal lamina of all epithelia, external laminae of muscle cells, adipocytes, shwann cells

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What is the function of ground substance?

traps and holds water, anchors, acts as a charge and size barrier, contributes to physical properties of a connective tissues, regulated morphogenesis, facilitates cell migration

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What is the importance of a ground substance’s ability to trap water?

fullness to skin
- protection
- reduces fluid loss
- limits pathogenic
invasion

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What is the importance of a ground substances ability to acts as a charge and size barrier?

- regulates access to cells

What is the importance of a ground substances ability to regulate morphogenesis?

- migration recognition | - growth factor activation

What are some general problems that can occur with issues within the ground substance?

Improper nutrient/waste management Improper tissue development (morphogenesis) Improper tissue growth (e.g. cartilage growth plate) Cellular malfunction due to GAG accumulation in lysosomes

What is a good example of a disorder of ground substance?

Hurler's syndrome

What is a family of diseases caused by accumulation of various GAGs in lysosomes?

mucopolysaccharides (MPS)

What are the three fiber types that are routinely stained for at the light microscope level?

1. reticular fibers which are argyrophilic i.e. stain with silver 2. elastic fibers that are best seen stained by special elastic stains (black stain) 3. collagen fibers which stain well with eosin (H&E) (red stain)

Why is it difficult to see type 1 collagen fibers in bone?

Because they are embedded in the ground substance

collagen has how many different types?

Each collagen type is made of different combinations of (blank)

alpha chains

Varying the alpha chain combinations of collagen dictates the (blank) properties of the different types of collagen fibers.

physical

What are the most common types of collagen?

I,II,III,IV

What is the most common of all collagens? | Where is it found?

Type I | Bone, skin, tendon, dentin, fibrocartilage

What does type I collagen originate? and what are its tissue properties?

oseteoblasts, fibroblasts, odontoblasts, chondroblasts. | Stiff, rope-like resists tension

Type I, II, and III collagen are all (blank) forming.

fibril

Where is Type III collagen (reticular fibers, highly glycosylated) found? Where does it originate? what is its tissue properties?

Forms structural framework of lymphatic tissue, adipose tissue, liver, cardiovascular system, lung, skin Originates:Fibroblasts, reticular cells, smooth muscle cells and hepatocytes Tissue Properties:Structural, delicate stroma (framework)

Where is type IV collagen found? where did it originate? What is the tissue properties like?

Basal lamina Epithelial cells, muscle cells, schwann cells Support and filtration of basal lamina

Which collagen type is network forming?

What type of collagen is highly glycosylated and known as reticular fibers?

collagen type III

What do you call defects in collagen synthesis?

collagenopathies

What can lead to Ehlers-Danlos type IV? | What are the symptoms?

faulty transcription or translation of type III collagen | aortic and/or intestinal rupture

What can lead to Ehlers-Danlos type VI? What are the symptoms?

Faulty lysine hydroxylation | augmented skin elasticity, rupture of eyeball

What can lead Ehlers-Danlos Type VII? What are the symptoms?

``` decrease in procollaen peptidase activity increased articular mobility (ability to move joints) frequent luxation (displacement of joints) ie rubber band joints ```

What can lead to scurvy? what are the symptoms?

Lack of vitamin C (cofactor for proline hydroxylase) | ulceration of gums, hemorrhages

What can lead to osteogenesis imperfecta? What are the symptoms?

change in one nucleotide in genes for collagen type I -Spontaneous fractures, cardiac insufficiency

(blank) are composed of elastin and microfibrils (fribillin).

elastic fibers

(blank) is a family of proteins which provides the scaffolding for the deposition of the elastin core. Mutations in the fibrillin gene results in (blank).

Fibrillin | Marfan syndrome

What is an amorphous material that imparts a high degree of elasticity characteristic of elastic fibers. These fibers may be stretched to 150% of their resting length without breaking. Their elasticity is due to the protein elastin. and their stability is due to the presence of microfibrils.

elastin core

What makes up the entire elastic fiber?

microfibrils and elastin core

what makes up the microfibrils?

fibrillin

What can be organized into groups, networks, or sheets/membranes (allowing for uniform dilation and constriction as in blood vessels.) ?

elastic fibers

Where can elastic fibers be synthesized?

fibroblasts, smooth muscle cells, and chondrocytes

When you see black fibers what can you assume they are?

elastic fibers.

(blank) is a systemic disorder of connective tissue with a high degree of clinical variability. Cardinal manifestations involve the ocular, skeletal, and cardiovascular systems. People are at increased risk for retinal detachment, glaucoma, and early cataract formation. The skeletal system involvement is characterized by bone overgrowth and joint laxity. The extremities are disproportionately long for the size of the trunk (dolichostenomelia). Overgrowth of the ribs can push the sternum in (pectus excavatum) or out (pectus carinatum). Scoliosis is common and can be mild or severe and progressive. The major sources of morbidity and early mortality are related to the cardiovascular system.

marfan syndrome

Stroma of the lymph node stained with silver are what kind of fibers?

reticular fibers

What are the types of fixed (resident) CT cells?

``` Fibrocytes (Chondrocytes, Osteocytes) Fibroblasts (Chondroblast, Osteoblast) Adipose Cells Mast Cells Macrophages (Osteoclast) Pericytes -stem cells for CT -role in angiogenesis & hypercellular obesity ```

What are the types of free (transient) cells?

``` Plasma cells Lymphocytes Neutrophils Eosinophils Basophils Monocytes Macrophages ```

what are the major producers of fibers and ground substance in connective tissues. Depending on the environment, what other cells can produce fibers and ground substances?

fibroblasts osteoblasts and chondroblasts

(blank) are euchromatic (i.e. their DNA is constantly being replicated) as are "blast" forms of other cells. They are primarily found in embryonic tissue and sites where formed connective tissue has been traumatized and undergoing repair.

fibroblasts

(blank) are heterochromatic so their nuclei stain very dark. They are capable of maintaining formed connective tissue.

Fibrocytes

Where do you find plasma cells?

scattered throughout connective tissues. Present in greatest number in chronic infammation and where foreign subtances or microorganisms have entered the tissues.

What are plasma cells derived from?

B lymphocytes

What do plasma cells do? | What kind of nucleus do they have?

When they have been activated with antigen, produce and secrete antibodies. clock face

(blank) are derived from circulating monocytes. They differentiate upon entry into connective tissue.

Macrophages

What are the two varieties of macrophages?

fixed and free

Are macrophages part of the mononuclear phagocytic system (MPS)?

yes

What are these characteristics of? Phagocytic or Antigen Presenting Cell Phagocytosis may be immune or non-immune mediated

macrophages

macrophages, osteoclasts,microganglia, langerhans cells are all part of the (blank)

mps

what does a mast cell look like?

granulated with an eyeball

What does this describe: They are derived from progenitor cells in the bone marrow. Possess granules containing histamine, heparin and others considered to be primary mediators They degranulate with secondary exposure to an allergen Capable of synthesizing secondary mediators

mast cells

what is the primary response of mast cells? | What is the secondary response?

IgE binding to Fc receptors Secondary Response Antigen crosslinks IgE molecules Granules released (primary mediators) Secondary mediators released

What do white fat cells look like?

look clear and white with red nucleus along outsides like a ring, unilobular

what does a white fat cell function in?

functions include calorie storage, insulation, protection, hormone production (leptin and adiponectin)

When are the number of fat cells determined?

perinatally (before birth)

Is distribution of white fat cells genetically determined and gender specific?

yes

What are some tumors of white fat cells?

lipomas, liposarcomas

white fat cell distribution is associated with various (blanks) succh as diabetes (males), heart disease (males) and breast cancer (females)

diseases

Where do you find brown fat cells?

around the neck and inter-scapular region

What does this describe: Multilocular cells with abundant cytoplasm filled with great numbers of lipid droplets and mitochondria; the later responsible for the brown gross appearance

brown fat cells

(blank) have a different unilocular form of brown fat

adults

(blank) fibers are associated with brown fat. White fat only have (blank) associated with ONLY their blood vessels.

nerve | nerve

What Functions in thermogenesis by oxidation of fatty acids. Thermogenins allows for the uncoupling of oxidation from phosphorylation. Heat is made instead (of ATP) which is then transferred to capillaries.

fat cells-brown

where is dense regular connective tissue (type I collagen) found?

areas where tension is exerted in a single direction (tendons, aponeuroses, ligaments)

What are cords attaching muscles to bones?

tendons

What are flat sheets attaching muscles to bones or to other muscles?

aponeuroses

What attach bones together at joints and contain more elastic fibers then do tendons therefore more stretchy?

ligaments

in a vangesen stain, what stains red, with small spaces of white in between which have elongated nuclei?

collegen

Where is dense irregular connective tissue found?

where tension is exerted in many directions. i.e. skin, heart valves,Fibrous pericardium, Perichondrium (membrane around the surface of cartilage), Periosteum (membrane around the surface of the bone), Fibrous capsules of organs and of joints.

where in the skin is dense irregular tissue found?

in the deeper layer of dermis and wall of digestive track. (forms submucosa.)

``` serves as a cushioning layer throughout the body often associated with adipose tissue ... What does this describe? ```

loose connective tissue.

what connective tissue is flexible, well vascularized, has little tensil strength and can be found easily in hypodermis and lamina proprea?

loose connective tissue.

what color do elastin fiber apear as in a veroeff slide, and what color does collgen appear?

black | red

In the renal sinus, islands of (blank) are often surrounded by white adipose tissue, which emphasizes the different appearances of the two tissue types. In brown adipose tissue, the nuclei of adipocytes are (blank). In addition capillaries are very frequent.

brown adipose tissue | are round and located more or less centrally in a cytoplasm

In an umbilical cord, what can you see?

large vessels, mucoid conective tissues (whartons jelly), simple squamos epithelium.

In mesenchymal connective tissue can be found in what?

dental pulp and in a fetal kidney

1000 Histo Connective Tissue Flashcards

(98 cards)