Basic transcriptional control mechanisms

Question 1

This type of response in gene expression is DEPENDENT on the continued presence of inducing signal

Answer 1

Type A

Question 2

This type of response in gene expression is TRANSIENT despite the continued presence of the signal

Answer 2

Type B

Question 3

This type of response in gene expression PERSISTS and is IRREVERSIBLE even after the termination of the signal

Answer 3

Type C

Question 4

These regulators in transcription influence gene expression only on the same chromosome

Answer 4

Cis-acting elements

Question 5

This is a group of prokaryotic genes in a linear array involved in a metabolic pathway

Answer 5

Operon

Question 6

What is the Negative regulator of the Lac Operon? What is its Positive regulator?

Answer 6

Negative: Lac I
Positive: CAP-cAMP

Question 7

True or False: >2% of the DNA is coding

Answer 7

False

Question 8

The activated Lac Operon stimulates the activity of which enzymes?

Answer 8

Beta Galactosidase and Lactase Permease

Question 9

These genes in eukaryotes are ‘constitutively’ expressed

Answer 9

Unregulated genes

Question 10

This is the region of DNA that signals for initiation, elongation and termination . It produces pre-mRNA from the template strand

Answer 10

Transcription Unit

Question 11

DNA region where RNA polymerase will bind to initiate transcription

Answer 11

Promoter Region

Question 12

TATA box in prokaryotes

Answer 12

Pribnow Box

Question 13

TATA box in eukaryotes

Answer 13

Hogness Box

Question 14

Position of TATA box in eukaryotes

Answer 14

-25

Question 15

POsition of TATA box in prokaryotes

Answer 15

-10

Question 16

Which of the following proteins are associated with Initiating the binding to the promoter site? Which is for termination?
A. Sigma Factor
B. Rho Factor

Answer 16

A. B. Respectively

Question 17

Aside from identifying the part of DNA to start transcription at, what is the other purpose of promoters?

Answer 17

Frequency of RNA synthesis

Question 18

DNA sequences that facilitate transcription (different from promoters)

Answer 18

Enhancers

Question 19

The only eukaryoic transcription factor that binds to DNA

Answer 19

TFIID

Question 20

Transcription factors that stabilize the initiation complex

Answer 20

TFIIA and B

Question 21

Transcription factor structurally and functionally similar to the Sigma Factor

Answer 21

TFIIF

Question 22

Components of TFIID

Answer 22

TBP and TAF