Required Practical 1 Flashcards
When is a line graph used in this practical
To present temperature, PH, enzyme concentration or substrate concentration
When should a bar chart be used in this practical
To measure the effect of an inhibitors
Other than temperature what are two other variables that’s should be controlled in this practical
Volume of amylase
Volume of Ph
How should the student monitor the temperature in this practical
Use a thermometer in the water bath and check the temperature every 10 minutes to make sure it stays constant in
Why would rate of starch hydrolysis would be faster at 40 degrees
Starch and amylase have more kinetic energy at higher temperature
More successful collisions
More enzyme substrate complexes
What are the factors that affect enzyme activity ?
Enzyme concentration
Substrate concentration
Temperature
Ph
How is a control set up in a practical measuring enzyme activity
Replace the enzyme solution with distilled water or boiled enzyme solution
How can the results be used to measure the initial rate of reaction
Plot your results on a graph of rate of reaction against time
Draw a tangent at time = 0 to find the initial rate
Outline the practical procedure used to measure the effect of temperature on enzyme activity using trypsin and milk ?
Immerse equal volumes of trypsin and milk stored in different test tubes in a water bath for 5 minutes
Until temperature at equilibrium
Mix together and immediately start timing
Record time taken for the milk to be completely hydrolysed
Test atleast 5 temperatures with 3 repeats
Rate of reaction calculation
1/time
What is the effect of temperature on enzyme activity
As temperature increases kinetic energy increases so more ez complexes form.
After optimum temperature, bond in the enzymes tertiary structure break which changes the shape f the active site
Substrate and active site no longer complementary
Rate of reaction decreases
