Microscope Info Flashcards
1
Q
Microscope invention
A
- Zachariah Janssen + Hans (father)
- Compound microscope (2 lenses)
2
Q
Francesco Redi/1668
A
- Experimented to test hypothesis
- Disproved spontaneous generation in maggots
3
Q
John Needham/1745
A
Tried to prove SG
Attributes results to LIFE FORCE
4
Q
Louis Pasteur/1859
A
- disproved SG
- used swan-shaped flask to show if no microbes enter nun grows
5
Q
Resolution
A
- ability to distinguish individual objects
- greater magnification = objects less resolved
6
Q
Contrast
A
- dark vs bright
- phase contrast microscopes enhance contrast
- makes cells clearer
7
Q
Staining
A
- increases contrast
- shows specific cell parts
8
Q
Compound Light Microscope
A
- light passes through specimen
- two lenser magnify image
- um used for measuring
9
Q
micrometers
A
- um
- 1mm = 1000 um
- used for small objects
10
Q
Magnification
A
- increases specimens apparent size
11
Q
Magnification formula
A
Ocular Lens Magnification X Objective Lens Magnification
12
Q
Field of View (FOV)
A
- circular region of slide visible under microscope
- higher magnification = smaller FOV
13
Q
FOV under low/medium/high power calculations
A
- measure diameter w ruler under microscope (low)
- ratios (med/high)
14
Q
FOV formula
A
FOV1 X MAG1 = FOV2 X MAG2
15
Q
Electron Microscope (EM)
A
- uses beams of electrons
- rly high resolving power
- magnify up to 1,000,000X
