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MICROBIOLOGY 2.0 > Corynebacteria. Listeria > Flashcards

Corynebacteria. Listeria Flashcards

0
Q

In what dye are the metachromatic granules highly stained

A

Aniline dye

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1
Q

Give the shape of corynebacterium

A

Rod, in parallel or acute angle with irregular swelling at one end

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2
Q

What do u call the irregular swelling in one end of coryne

A

Babes-Ernst bodies

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3
Q

True or false. C. Diph are resitant to heat and killed by drying

A

False. Killed by heat. Resistant to drying

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4
Q

How long can they stay in dust? C. Diph

A

102 days

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5
Q

C diph grows best on ——- environment

A

Anearobe at 37

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6
Q

They can be found where? C diph

A

Fresh and salt water soil and air

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7
Q

Their characteristic on blood agar

A

Small, granular, gray, irregular edges

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8
Q

Reduced tellurite is positive of what

A

Staph, strep, coryne

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9
Q

Give the rxn in tellurite agar

A

Brown to black with halo

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10
Q

This medium grows more coryne than other org

A

Loeffler

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11
Q

Other name for potassium tellurite

A

Modified tinsdale

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12
Q

True or False. All coryne subtypes are toxigenic.

A

False

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13
Q

What causes toxigenicity in c diph

A

Phage gene

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14
Q

Invasiveness due to

A

Bacterial gene

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15
Q

Resistant drugs to listeria

A

Fluoroquinolones

Cephaloaphorins

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16
Q

DOC for listeriosis

A

Ampicillin plus gentamicin

Erythro. Cotri

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17
Q

Diphteria is spread via

A

Droplet and contact trans

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18
Q

It desires low iron content for growth.

A

C diph

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19
Q

What level of iron does c diph growth is optimal and suppressed

A

Optimal 0.14

Supp 0.5

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20
Q

Diph toxin is heat labile. True or false

A

True

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21
Q

Which fragment of diph toxin binds to CD9 and HBEGF

A

Fragment b

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22
Q

This inhibits polypeptide chain elongation via ef 2

A

Fragment a

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23
Q

This is responsible for the neurotoxic and necrotizing effect of diph toxin

A

Abrupt cessation of protein synthesis

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24
Q

This is manifested as cervical adenitis and submandibular edema

A

Bullneck

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25
Q

Diphteria incubation period

A

2-6 days

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26
Q

How many weeks does the bcteria persist in untreated infxn

A

6 weeks

27
Q

With treated diph, they become less comm at what day/week

A

Less than 4 days

28
Q

Most common loc of virulence

A

Upper respi tract

29
Q

Exudates in nasal

A

Serosanguinous or seropurulent

30
Q

Doc for diph

A

Pen and ery x 14 days

31
Q

What co existing org is also killed with ery and pen?

A

Strep

32
Q

Elimination is documented when?

A

24 h post last say of meds. 2 cons negative, 24 h apart

33
Q

Tellurite can have definite colonies in how many hours?

A

36-48h

34
Q

Loeffler slant yields microbe in how many hours?

A

12-18h

35
Q

What does negative culture but positive PCR means?

A

Positive

36
Q

This test shows a precipitation line post 48hours at 45 degrees

A

Mod elek test

37
Q

Used to detect tox gene

A

Pcr

38
Q

What must be done prior to diph antitoxin administration? When must it be done?

A

Skin test. Same day of dx.

39
Q

Cutaneous diph are treated for how many days?

A

10 days and cleanse with soap and water

40
Q

Where is the primary lesion in diph

A

Throat

41
Q

Prophylaxis for diph

A

Ery x 10 days
IM Pen G, 1 dose
Active vaccination

42
Q

When does heart problem occurs?

A

When respi symptoms are improving at 2-3 weeks

43
Q

Neuro prob occurs when and involves what.

A

Soft palate, pharyngeal wall. Sensory and motor nerve.

44
Q

This coryne doesnt produce toxins.

A

Diphtheroids

45
Q

Diphteroids asso with cath infxns

A

C striatum

46
Q

Causes pharyngitis

A

C. Haemolyticum

47
Q

Causes skim ulcers

A

Bovis. Haemolyticum. Ulcerans

48
Q

Causes bacteremia and sepsis

A

Xerosis. Group D2, E, JK. Pyogenes. Bovis

49
Q

This virulence actor hydrolyses sphingomyelin promoting spread of what org

A

Phospholipase D. Diphtheroids

50
Q

This causes alkalinization of urine and leads to struvite formation

A

Urease

51
Q

Doc for diphteroids

A

PM FR

Pen. Macrolide. Fluoro. Rifam

52
Q

This culture media is used for listeria

A

Mueller Hinton agar

53
Q

This non spore forming, gram positive have peritrichous flagella

A

Listeria

54
Q

Motile and esculin positive non spore forming gram positive

A

Listeria

55
Q

What do u call the movement of listeria

A

Tumbling end over end motolity at 22-28

56
Q

True or false. Listeria grows in 4degree, low ph and high salt

A

True

57
Q

This virulence factor promotes phagocytosis. Cell surface protein

A

Internalin

58
Q

This promotes formation of filopods which are elongates protrusions

A

ActA

59
Q

This promotes the lysis of phagolysosome.

A

Listeriolysin O.

60
Q

True or false. This organism can move from cell to cell without being exposed to abs

A

True

61
Q

What happens to immunocompetent patients with listeria

A

Febrile gastroenteritis. Self limiting

62
Q

Occurs in immunocom with listeria

A

Meningo and bacteremia

63
Q

Disseminated form of listeria

A

Granulomatosis infantiseptica

64
Q

This listeria serotype causes late onset syndrome

A

Serotype ivb

MICROBIOLOGY 2.0 (7 decks)

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