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Bacte Lab (Midterm) > 1.1.1 Cultivation of bacteria > Flashcards

1.1.1 Cultivation of bacteria Flashcards

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1
Q

Process of growing microorganisms in culture by taking bacteria from in vivo (Infection site) to In vitro environment (Artificial environment)

A

Bacterial cultivation

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2
Q

What is in vivo environment in Bacterial cultivation?

A

Infection site

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3
Q

What is in vitro environment in Bacterial cultivation?

A

Artificial environment that mimics in vivo

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4
Q

What are the main purpose of Bacterial Cultivation

A

To grow and isolate
To determine which bacteria that grow that causes infection
To obtain sufficient growth of clinically relevant bacteria to allow Identification, characterization, and susceptibility testing

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5
Q

What are the nutritional needs for bacteria?

A

Carbon
Nitrogen
Non-metallic elements (Sulfur, phoshorus)
Metallic elements
Vitamins
Water
Energy

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6
Q

Most essential and central atom common to all cellular structures and functions

A

Carbon

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7
Q

Organisms requires inorganic compounds or carbon

A

Autotrophs (CO2)

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8
Q

Organisms requires organic compounds or carbon

A

Heterotrophs (Carbohydrates)

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9
Q

Essential atom for proteins and nucleic acid

A

Nitrogen

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10
Q

Type of proteins that is responsible for metabolic activities

A

Enzymes

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11
Q

Example of Non metallic elements?

A

Sulfur
Phosphorus

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12
Q

Integral to some amino acids and component of proteins

A

Sulfur

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13
Q

What is the source of sulfur for organic compounds

A

amino acids

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14
Q

What is the source of sulfur for inorganic compounds

A

Sulfates

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15
Q

Non metallic elements that forms the nucleic acid DNA and RNA

A

Phophorus

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16
Q

Phosphorus synthesize a high energy organic compound called?

A

Adenosine triphoshate

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17
Q

What is phosphorus being supplied to the cell?

A

Phosphate salt

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18
Q

What are the metallic elements

A

Calcium, Zinc, Sodium, Potassium, Copper, Manganese, Magnesium Ferrous, and Ferric

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19
Q

What are the activities for metallic elements?

A

Osmoregulation,
Regulation of enzyme activity,
Electron Transport

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20
Q

How is metallic elements present in the body?

A

Present in small amounts and are supplied by inorganic salts

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21
Q

Sources of coenzymes which are required for the formation of active enzyme systems

A

Vitamins

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22
Q

Vitamins are present in _____ amount and produce by?

A

Small
Organic substance

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23
Q

Universal medium requires to cross the cell membrane

A

water

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24
Q

Purpose of energy?

A

Active transport
Biosynthesis
Biodegradation of macromolecules

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25
Bacteria requires radiant energy
Phototrophs
26
Dependent on oxidation of chemical compounds
Chemotrophs
27
Nutrients are incorporated on or in which bacteria are grown?
Culture media
28
What are the consideration for culture media?
Growth requirements Optimal environmental conditions
29
Bacteria that needs relatively complex, and exceptional media components
Fastidious microorganisms
30
Bacteria that needs relatively basic and straightforward nutrients (Clinicaly important bacteria)
Non-Fastidious micoorganisms
31
NOTE:
Additional requirement nutrients require for the growth of fastidious microorganisms
32
What is the difference between broth and Plate
Concentration of agar
33
What is the basis for growth in broth/Culture media?
Turbidity
34
The higher the turbidity, ____________
more cell density
35
How many to know if the bacteria is in the broth?
10th to the 6th bacteria/mm of broth
36
What is the basis for growth of bacteria in agar?
Colonies
37
Composed of KNOWN quantities of chemically pure, specific organic and/or inorganic compounds. Their use requires knowledge of the organism's specific nutritional needs
Chemically defined media / Minimal media
38
Example of Chemically defined media?
Inorganic synthetic broth Glucose salts broth
39
The Exact chemical composition of these media is NOT KNOWN. They are made of extracts of plants and animal tissue and are variable in their chemical composition
Complex media
40
Example of complex media?
Nutrient agar Yeast Extract broth
41
This type of medium is not usually used in diagnostic microbiology laboratory
Chemically defined media (Minimal media)
41
This type of medium is not usually used in diagnostic microbiology laboratory
Chemically defined media (Minimal media)
42
Components of Nutrient agar
peptone (Nitrogen) Beef extracts (Organic carbon, vitamins, inorganic salts)
43
Contain specific nutrients required for the growth of particular bacterial pathogens.
Enrichment media
44
Example of Enrichment media?
Buffered charcoal-yeast extract agar (BCYE)
45
What bacteria can grow on BCYE?
Legionella pneumophila Nocardia
46
Used to ensure growth of an organisms when no organisms grow on solid media after initial specimen inoculation
Enrichment broths
47
Causative agent of Legionnaires disease
Legionella pneumophila
48
What contains in BCYE for the growth of Legionella pneumophila?
L-cystine
49
Example of Enrichment broth?
Thioglycollate broth Todd-hewitt broth Gram negative broth (GN broth)
50
What is the purpose of Thioglycollate broth?
Separation anerobic microorganisms
51
What is the component for Todd-Hewitt broth?
Colistin and Nalidixic acid
52
What bacteria grow on Todd-Hewitt broth?
Streptococcus agalactiae
53
What is the purpose of GN broth?
Enrich Enteric gram negative microorganisms
54
Contain nutrients that support growth of most non-fastidious organisms WITHOUT any particular organisms a growth advantage General purpose
Nutritive media
55
Example of Nutritive agar
Tryptic soy agar/broth Nutrient agar (Bacteria) Sabouraud's dextrose agar (Fungi)
56
Contain one or more agents that are inhibitory to all organisms except those "selected" by the specfic growth condition or chemica
Selective media
57
Examples of inhibitory agents
Dyes (Crystal violet) Bile salts Alcohols Acids Antibiotics BAlAcDAn
58
Example of Selective media?
Phenylethyl alcohol with 5% SBA
59
What is the purpose of PEA?
inhibits the growth of aerobic and facultatively anaerobic gram-negative rods
60
Employ some factor/s that allow colonies of one bacterial species or type to exhibit certain metabolic or culture characteristics that can be used to distinguish then from other growing on the same agar plate
Differential media
61
Example of Differential media?
MacConkey agar
62
Media used in diagnostic bacteriology that provide more than one function
Combination media
63
Differentiate lactose fermenter from non lactose fermenter
MacConkey agar
64
Positive indicator for MacConkey
Pink colonies
65
Negative indicator for MacConkey
Colorless
66
Components of MacConkey agar?
Bile salts Crystal violet - Inhibits Gram positive cocci

Bacte Lab (Midterm) (9 decks)

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