EX. Biochemical Test Results

Question 1

What are the Extracellular Enzymes?

Answer 1

Starch
Lipid
Casein
Gelatin hydrolysis

Question 2

What is the Result for starch hydrolysis?

Answer 2

Clear zone (+)
Bacillus cereus

Blue coloration (-)

Question 3

What is the result for Lipid hydrolysis

Answer 3

Clear zone (+) S. aureus

No clear zone (-)

Question 4

What is the result for Casein hydrolysis?

Answer 4

Clear zone (+) Bacillus cereus

No clear zone (-)

Question 5

What is the result for Gelatin hydrolysis?

Answer 5

Liquifies (>25 C, +), Proteus vulgaris

Solidifies (<25 C, -), Pseudomonas aeruginosa

Question 6

What are the extracellular enzymes for Enterobacteriaceae?

Answer 6

Oxidase Test
Nitrate Reduction
Nitrite Reducatio test
Ortho-nitrophenyl-Beta-D-Galatopyranoside
Amino Acid Utilization
- Lysine > Cadevarine (Decarboxylase)
- Ornithine > Putresine (Decarboxylase)
- Arginine > Citrulline (Dehydrolase)
+ Moeller Decarboxylase medium
Sulfide Indole Motility
Carbohydrate fermentation

Question 7

What is the result for Oxidase test?

Answer 7

Tetramethyl-P-phenylene diamine - dihydrochloride

Purple (+) PPAM ViC HeN
Colorless (-) Enterobacteriacheae

Question 8

What is the Result for Nitrate Reduction test?

Answer 8

Alpha-Naphthylamine & sulfanilic acid
Red (+) Enterobacteriaceae

NO3 > NO2

Question 9

What is the Result for Nitrite Reduction tst?

Answer 9

Colorless (+) E. faecalis

Negative (-) Red

Alpha-Naphthylamine + Sulfanilic Acid + Zinc dust

Question 10

What is the Result for Ortho-Nitrophenyl-Beta-D-Galactopyranoside?

Answer 10

Yellow (+) for lactose fermenter (Usually detects early Lactose fermenter)

Question 11

What is the Final Product of Lysine?

Answer 11

Cadaverine (Decarboxylase)

Question 12

What is the Final Product of Ornithine?

Answer 12

Putrescine (Decarboxylase)

Question 13

What is the Final product of Arginine

Answer 13

Citrulline (Dihydrolase)

Question 14

What is the result for Moeller Decarboxylase Base Medium?

Answer 14

(+) purple Klebsiella pneumoniae

Question 15

What is the result for Moeller Decarboxylase Base Medium?

Answer 15

(+) purple Klebsiella pneumoniae
(-) Yellow Enterobacter cloacae

Question 16

What is the negative control for Lysine Decarboxylase?

Answer 16

Enterobacter cloacae

Question 17

What is being added in Fermentation for?

Answer 17

Mineral oil (Anaerobic) = Yellow (Acid)

Question 18

Bacteria that are positive in Arginine?

Answer 18

E. cloacae
E. sakazakii

Question 19

What are the bacteria from SPACEd that cause blackening?

Answer 19

Salmonella
Proteus
(Salmonella) Arizonae
Citrobacter freundii
Edwardsiella tarda

Question 20

What is the result for Motile in Sulfide Indole Motility?

Answer 20

Bruch like appearance or Turbidity in the inoculation site

Question 21

Use to Detect gas production

Answer 21

Durham Tube

Question 22

What are the sugars in TSI and it’s percentage?

Answer 22

S 10%
L 10%
G 1%

Question 23

What is H2S indicator for TSI?

Answer 23

Ferrous Sulfate and Sodium Thiosulfate

Question 24

What are the CHYSSA

Answer 24

Citrobacter freundii
Hafnia
Yersinia enterocolitica
Shigella sonnei
Serretia mercesens
Arizonae

Question 25

From SPACEd, what is the result for the bacteria with A/A+H2S?

Answer 25

Citrobacter freundii Arizonae

Question 26

From SPACEd, what is the result for the bacteria with K/A+H2S?

Answer 26

Salmonella Proteus Edwardsiella tarda

Question 27

From SPACEd, what is the bacteria with A/A+H2S + K/A?

Answer 27

Citrobacter freundii

Question 28

From SPACEd, what is the bacteria with A/A+H2S + K/K?

Answer 28

Arizonae

Question 29

From SPACEd, what is the bacteria with K/A+H2S + K/A?

Answer 29

Salmonella

Question 30

From SPACEd, what is the bacteria with K/A+H2S + K/K?

Answer 30

Edwardsiella tarda

Question 31

From SPACEd, what is the bacteria with A/A+H2S + R/A?

Answer 31

Proteus spp

Question 32

From HYSS, What is the bacteria with K/K

Answer 32

Hafnia Serretia marcescens (End - End)

Question 33

From HYSS, What is the bacteria with K/A

Answer 33

Yersinia Enterocolitica Shigella Sonnei

Question 34

What is being detected in Indole test?

Answer 34

Tryptophanase

Question 35

What is the reagents?

Answer 35

Kovaks or Erlich (p-dimethyaminobenzaldehyde)

Question 36

What is the End product of Methyl Red?

Answer 36

Mixed Acid (Acetic acid, Lactic acid, Formic Acid)

Question 37

What is the media used in Methyl Red?

Answer 37

Clark and Lubs medium

Question 38

What is the positive indicator for ,MR?

Answer 38

Red 4.4

Question 39

What are the 2 methods for Vogues Proskauer?

Answer 39

Barrits Coblentz method

Question 40

Barritz method Reagents?

Answer 40

40% KOH and 5% alpha naphthol

Question 41

Coblentz method reagents?

Answer 41

40% KOH with creatine and 5% alpha naphthol

Question 42

What are the positive bacteria in VP?

Answer 42

KESH Klebsiella (Except for Ozanae and rhinscleromatis) Enterobacter Serretia marcescens Hania alvei

Question 43

Bacteria that are positive in Simmons Citrate?

Answer 43

Enterobacter Klebsiella Citrobacter Pseudomonas aeruginosa

Question 44

What is Positive in Acetate utilization?

Answer 44

E coli (Blue) Shigella flexneri (Green)

Question 45

What s positive in MUG Test?

Answer 45

E. coli blue Pseudomonas aeruginosa no flurescens

Question 46

What bacteria are positive in Urease?

Answer 46

E. gergoviae K. pneumoniae K. oxytoca Citrobacter spp Serretia marcescens Yersinia pseudotuberculosis Yersinia enterocolitica PPM

Question 47

In urease, what species of Providencia is Negative?

Answer 47

Providencia alcalifasiens

Question 48

In PPM, who is positive in Ornithine?

Answer 48

Morganella morgonii

Question 49

What is the presumptive identification of PPM?

Answer 49

Pheylalanine Deaminase

Question 50

Positive for Phenylalanine Deaminase?

Answer 50

Green

Question 51

What is the nonoxidizer or non utilizer in O/F?

Answer 51

Alcaligenes faecalis