Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology > Lab Exam 2 > Flashcards

Lab Exam 2 Flashcards

1
Q

Acidophiles optimum ph

A

pH 3

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Neutrophile optimum pH

A

pH 7

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Alkaphile optimum pH

A

pH 9-10

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Salt tolerance of non-halophile

A

<1%

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Salt tolerance of Halo tolerant bacteria

A

0.5%-2%

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Salt tolerance of halophile

A

3%-30%

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Temperature tolerance of psychrophiles

A

~10C optimum

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Temperature tolerance of psychrotroph

A

~25C optimum

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Temperature tolerance of mesophile

A

~37C optimum

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Temperature tolerance of thermophile

A

~60C optimum

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

A highly acidic or basic medium is selective for what kind of bacteria

A

Bacteria with extreme pH tolerance

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

A highly salty medium will select for what kind of bacteria

A

Salt tolerant bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Salt tolerance bacteria can be found in what environments (4)

A

Brackish water, ocean water, brine water, saturated solutions in the lab

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Obligate anaerobes…

A

Require no oxygen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Aero tolerant bacteria…

A

Do not use oxygen but can live with it present

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Facultative anaerobes…

A

Can use oxygen but do not require it

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Explain a catalase test.

A
  • Drop of hydrogen peroxide + glob of fresh bacteria
18
Q

Explain catalase test results.

A
  • bubbles = catalase pos +

- no bubbles = catalace neg -

19
Q

Explain an Oxidase test.

A
  • Glob of fresh bacteria on cotton swab + Reagent
20
Q

Explain Oxidase test results

A
  • Purple immediately = pos for oxidase +

- no color change = neg for oxidase -

21
Q

Explain a Nitrate broth test.

A
  • inoculate broth with loop
  • add reagents A and B
  • if inconclusive add zinc
22
Q

Explain nitrate broth test results.

A 
Red= nitrite present + for anaerobic respiration
Clear = inconclusive Add Zinc
Red = nitrate present - for nitrate reduction
Clear = nitrate gone + positive reduced further to nitrogen
23
Q

Explain an O/F Deep test.

A
  • Stab with needle to inoculate two green tubes

- add oil to one (anaerobic tube)

24
Q

Explain test results of O/F Deep test

A
  • (yellow)acid production + oxygen = respiration
  • (yellow) acid production + NO oxygen = fermentation
  • green = no carbohydrates we’re used.
25
Q

Explain SIM Deep test.

A

Stab with needle to inoculate sim deep media

26
Q

Explain test results for SIM Deep test.

A

Black = hydrogen sulfide production

Growth top only = obligate anaerobe
Growth throughout= aero tolerant or facultative
Growth at bottom only = obligate anaerobes

Stays in stab = nonmotile
Cloudy around stab = motility

Kovacs reagent –> red = tryptophanase made

27
Q

Explain an indol test.

A

Glob of bacteria on cotton swab + drop of reagent

28
Q

Explain indol test results.

A

Pink = tryptophanase +

Indol made as a byproduct of tryptophan break down.

29
Q

Explain a Thioglycollate Deep test.

A

Stab Thioglycollate media to inoculate

30
Q

Explain Thioglycollate Deep test results.

A

Growth on top= obligate anaerobe
Growth throughout= aero tolerant
Growth throughout but heavy near top= facultative
Growth bottom only= obligate anaerobe

31
Q

How do you inoculate Deep Tubes?

A

By stabbing with needle

32
Q

What are Durham Tubes used for?

A

To check for gasses

33
Q

What is the use of oil or gas -pak bags used for?

A

To keep media free of oxygen

34
Q

Which process gives more energy per glucose molecule? Respiration or fermentation?

A

Respiration

35
Q

What could cause an Oxidase test to show a false pos or neg?

A

High levels of glucose can give false neg reactions. Colonies must be grown on menus with no sugars.

36
Q

Why does Thioglycollate media contain a red color indicator

A

To show an aerobic to anaerobic gradient.

37
Q

What type of microbes are expected to contain Oxidase?

A

Aerobic and facultative bacteria

38
Q

Which type of bacteria is catalase expected to be found in?

A

Anaerobes and facultative

39
Q

What is TMPD?

A

A chromogenic reducing agent.

40
Q

When TMPD is oxidized what color does it turn?

A

Blue

Microbiology (4 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions