Summary of Gene Technology

Question 1

What is the first step of making a human copy of a protein in gene techology?

Answer 1

isolate the target gene - using restriction enzymes

Question 2

What is the second step of gene technology?

Answer 2

insert the target gene into vector - need to use the same restriction enzyme, sticky ends to be complementary, use DNA ligase to make phosphodiester bonds, and you form recombinant DNA

Question 3

What is the third step of gene technology?

Answer 3

insert the vector into a bacteria - use ice cold calcium chloride and heat shock and electroportation, which forms a
trasnformed organism

Question 4

What is the fourth step of gene technology?

Answer 4

identify the transformed organisms - use a marker gene to target gene e.g.radiactivity / antibiotic resistance

Question 5

What is fifth step of gene technology?

Answer 5

culture the transformed bacteria - bacteria will have machinery to transcribe and translate the target gene - to make the protein

Question 6

What is sixth step of gene technology?

Answer 6

extract and purify the protein