Protein sequencing Flashcards by Adam Sleiman | Brainscape

Q

N terminal determination methods…(3)

A

Amino-peptidase(exo)
DNFB(sanger), DABSYL, DANSYL
EDMAN degradation > PTC + hydrolysis + release

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

C terminal determination methods…()

A

Carboxy-peptidase(exo)
Hydrazinolysis
Endopeptidases
> Trypsin…in basic A.A.(arg, lys)
> Chymotrypsin…in aromatic A.A.
> Pepsin…non-specific
CNBr…at met

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

… identifies location of disulfide bridges

A

Diagonal electrophoresis

How well did you know this?

1

Not at all

2

3

4

5

Perfectly