Lecture 2 Flashcards
What are the other names of 7 transmembrane receptors
7 pass transmembrane receptors
G protein coupled receptor
What are the terminals in the extracellular side and cyrosol side
Extra = amino terminal
Cytosol = carboxy terminal
Where do the ligand bind to the receptor
In the extracellular side of the receptor
How many sub-unit in G protein and what do they called
3 sub-units
Alpha , beta , gamma
Explain the process of GPCR amplification
1- Adrenalin bind to its receptor ( beta adrenerginic receptor )
2- then GDP will be converted to GTP
3- GTP will bind to the alpha sub-unit which will dissociate from the other sub-units
4-then this complex ( GDP+alpha subunit ) will bind to the enzyme ( Adenylyl cyclase )
5- the enzyme will catalyse the formation of cAMP which will activate protein kinase A
6- PKA will phosphorylate the cellular protein and make the cell response to adrenaline
What are the acceptors that are regulated by PKA ?
Structural proteins , enzyme , ion channel , transcription factors
Phospholipids C can be activated by what ?
G protein and phosphorylation of tyrosine
Explain the pathway of activation of PKC ?
Phospholipase C cut the molecule into diacyglycerol and iP3 DAG will activate Protein kinase C and iP3 will allow the release of Ca+ from Endoplasmetic reticulum which then will further activate Protein kinase C
Protein kinase C can be activated ted by ?
Ca+ and diacyglycerol
What are the molecules that can be activated by Ca+ directly ?
Protein kinase C , phospholipase A2 and DNAses
What are the molecules that can be activated by Ca+ indirectly ?
Calmodulin
Calmodulin regulates ?
Glycogen phosphorylase kinase , Muscle contraction and calmodulin regulated protein kinase
RTk how many in humans ?
- , 20 sub-families
