Q1: Factors Affecting Enzymatic Activity

Question 1

The enzymes become denatured if the temperature is _______ compared to its optimum temperature.

Answer 1

Too High

Question 2

What happens when an enzyme changes shape?

Answer 2

Reaction slows down

Question 3

True or False

Enzymes work best within specific temperature and pH ranges, and sub-optimal conditions that can cause an enzyme to lose its ability to bind to a substrate.

Answer 3

TRUE

Question 4

5 Determinants of the Factors Affecting Enzyme Activities

Answer 4

Temperature, pH, Substrate Concentration, Enzyme Concentration, and Effect of Activators

Question 5

Raising temperature generally __________ a reaction, and lowering temperature __________ a reaction

Answer 5

Speeds up, Slows down

Question 6

extreme high temperatures can cause an enzyme to _________ and stop working

Answer 6

Denature

Question 7

TRUE OR FALSE

Most enzymes have an optimum temperature (temperature at which a procedure is best carried out on the action of an enzyme), near normal body temperature at which they catalyze a reaction most rapidly.

Answer 7

TRUE

Question 8

pH abbreviation for ______ or ______.

Answer 8

Power of Hydrogen/Potential of Hydrogen

Question 9

_________ pH values can cause enzymes to denature.

Answer 9

Extreme

Question 10

TRUE or FALSE

Small pH changes does not affect the electrical charges on various chemical groups in enzyme molecules, thereby altering the enzyme’s ability to bind its substrate and catalyze a reaction.

Answer 10

FALSE

Even small pH changes can alter the electrical charges on various chemical groups in enzyme molecules, thereby altering the enzyme’s ability to bind its substrate and catalyze a reaction.

Question 11

Enzymes catalyze a reaction most rapidly at an _________, near _______.

Answer 11

optimum range, neutral

Question 12

Increasing substrate concentration, _________ the rate of reaction to a certain point.

Answer 12

Increases

Question 13

TRUE OR FALSE

Once all of the enzymes have bound, any substrate increase will have no effect on the rate of reaction, as the available enzymes will be saturated and working at their maximum rate.

Answer 13

True

Question 14

At the _____________, the reaction will not speed up, no matter how much additional substrate is added.

Answer 14

Saturation Point

Question 15

The graph of reaction rate is plateauing.

Answer 15

Substrate Concentration

Question 16

Increasing enzyme concentration will _______ the reaction, as long as there is substrate available to bind to.

Answer 16

Speed up

Question 17

TRUE or FALSE

The higher the concentration of an enzyme the greater should be the initial reaction rate. This will last as long as there is an infinite amount of substrate present.

Answer 17

TRUE

Question 18

The graph of reaction rate is linear

Answer 18

Enzyme Concentration

Question 19

The graph of reaction rate is Bell-shaped.

Answer 19

pH

Question 20

TRUE or FALSE

Some of the enzymes do not require certain inorganic metallic cations, like Mg2+, Mn2+, Zn2+, Ca2+, Co2+, Cu2+, Na+, K+ etc., for their optimum activity. Rarely, anions are also needed for enzyme activity, e.g. a chloride ion (CI–) for amylase.

Answer 20

FALSE

Some of the enzymes require certain inorganic metallic cations, like Mg2+, Mn2+, Zn2+, Ca2+, Co2+, Cu2+, Na+, K+ etc., for their optimum activity. Rarely, anions are also needed for enzyme activity, e.g. a chloride ion (CI–) for amylase.