4.7.5.1 Lab diag

Question 1

What is the medium use for swabs?

Answer 1

Cary-Blair medium

Question 2

What reagent is use for stool collection?

Answer 2

Alkaline peptone water (8.5 pH for stool culture)

Question 3

Medium that causes, medium to large, smooth, opaque, iridescent with greenish hue

Answer 3

BAP and CAP

Question 4

MAC reaction?

Answer 4

Colorless except V. vulnificus

Question 5

MAC reaction for V. vulnificus?

Answer 5

Pink because it can utilize lactose

Question 6

In TCBS, what is the function of Thiosulfate and sodium citrate?

Answer 6

Inhibits Enterobacteriaceae

Question 7

In TCBS, what is the function of Bile salt?

Answer 7

Inhibits Gram positive

Question 8

In TCBS, what is the pH indicator?

Answer 8

Bromothymol blue

Question 9

In TCBS, what is the function of NaCl?

Answer 9

provide optimum growth and metabolic activity

Question 10

In TCBS, what bacteria has growth with fermentation (Yellow)?

Answer 10

V. cholerae
V. alginolyticus
V. fluvialis
V. furnissi
V. cincinnatiensis
V. metschnikovii

Question 11

In TCBS, what bacteria has growth without fermentation (green/blue-green)?

Answer 11

V. mimicus
V. parahaemolyticus
V. damsela
Most of V. vulnificus strains

Question 12

In TCBS, what bacteria has No growth?

Answer 12

Aeromonas
Plesiomonas
Chromobacterium

Question 13

NOTE:

Answer 13

V. cholerae O1 and O139 causes epidemic cholera but O1 is more potent in creating the epidemic

V. cholerae non-O1 less potent