quiz 6

Question 1

Whats the main difference between agarose and agropectin?

Answer 1

agarose is neutral charged and agaropectin is negatively charged.

Question 2

What is the purpose of using SWC media with agar-degrading bacteria?

Answer 2

TO see if they do catabolite repression, using nutrients in a rich media instead of agar(looking for a decrease in agarase)

Question 3

What does nutritionally fastidious mean?

Answer 3

Picky, require many things to grow successfully

Question 4

What are lactic acid bacteria morphology?

Answer 4

gram positive cocci

Question 5

What do we expect to see on the TSY plate?

Answer 5

It is trp +, we expect to see no growth. Growth on this plate due to unlysed donor cells in the solution

Question 6

What are we looking for In our purple non sulfur wet mounts?

Answer 6

motile, helical shaped cells-rhodospirillum
long, irregular rods with swollen tip-rhodopseudomonas

Question 7

What is chain termination in PCR?

Answer 7

incorporates flourescently labelled nucleotides that have no 3’OH and cannot add another base afterward. Only one primer added, only s single DNA synthesized