quiz 8 Flashcards

1
Q

methyl red test

A

indicates mixed acid fermentation pathway, red is positive, yellow is negative, orange is inconclusive or negative

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2
Q

VP/acetoin test

A

butanediol fermentation pathway to ferment glucose, positive is red, no color is negative

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3
Q

citrate test

A

capability of utilizing citric acid as sole carbon source, visible growth is positive and sometimes blue

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4
Q

ornithine test

A

presence of enzyme ornithine decarboxylase. purple is positive, yellow is negative

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5
Q

What is the morphology of enterobacteria?

A

facultativ anaerobes, gram negative rods, oxidase negative, motile

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6
Q

What is the physical appearance of E.coli on EMB agar?

A

irregular, flat, almost black colonies with green sheen

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7
Q

Why use the lactose mineral salt?

A

To restrict streptococcus and micrococcus

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8
Q

Why were propionic bacteria able to survive our skin surface?

A

They’re aeroduric

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9
Q

On a sourdough TSY plate, what do yeast look like?

A

large, irregular, white colonies

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10
Q

What is the morphology of our LA bacteria?

A

gram positive, catalase negative

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11
Q

What does a cloudy/turbid plaque assume?

A

Incomplete lysis, temperate phage

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12
Q

what is the job of the repressor?

A

Deactivates sigma gene expression

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13
Q

How does sigma phage grow on E.coli?

A

partial growth bc no repressor of sigma genes

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14
Q

How does sigma phage grow on sigma E.coli?

A

No growth bc sigma phage repressor turns off sigma gene expression, lysogen immunity

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15
Q

What is lysogen immunity?

A

conferred protection against phage of same kinda as prophage

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16
Q

what is sigma vir?

A

Altered operator region where sigma repressor binds, no repression

17
Q

Why is there no growth on lambda?

A

no phage receptors so cannot recognize the cell and grow

18
Q

What is the ourpose of the K12 E.coli plate?

A

demonstrate the difference between turbid and clear plaque

19
Q

what are the white micro colonies on clear plaques?

A

mutant e.coli strains that no longer express the lambda receptor protein, they are resistant to lambda infection because phage can no longer recognize and adsorb cell

20
Q

with PNS bacteria, what do g neg rods and spills signify/

A

rods are rhodobacter and rhodopseudomonas, spirals are rhospirillium