Histopath

Question 1

The process of applying the dyes on the
section to see the architectural pattern of tissue and physical characteristic of cells

Answer 1

Staining

Question 2

Commonly used type of microtome for routine in cutting paraffin embedded section.

Answer 2

Rotary microtome

Question 3

Commonly used fixative agent

Answer 3

10% NEUTRAL BUFFERED FORMALIN

Question 4

Ideal thickness of tissue in sectioning

Answer 4

4mm

Question 5

Branch of science that deals with the gross and microscopy study of tissues

Answer 5

Histology

Question 6

Type of anatomic pathology that deals with study of tissue organs and tumors.

Answer 6

Surgical pathology

Question 7

Type of pathology that plays a critical role in determining an accurate diagnosis.

Answer 7

Anatomical pathology

Question 8

Commonly used type of dehydrating agent

Answer 8

Ethyl alcohol

Question 9

Commonly used for impregnating tissue

Answer 9

Paraffin wax

Question 10

Desired temperature of cryostat machine for frozen section

Answer 10

-18 TO -20 DEGREE CELSIUS

Question 11

Give FIVE (5) important steps in Tissue
processing.

Answer 11

1. FIXATION
2. DEHYDRATION
3. CLEARING
4. IMPREGNATION
5. SECTIONING

Question 12

Give at least TWO (2) important role of
fixation.

Answer 12

AVERT AUTOLYSIS, OR THE DECAY OF TISSUE DUE TO THE ACTION OF CELLULAR ENZYMES AND PUTREFACTION, OR BACTERIAL ATTACK

Staining is enhanced by fixation

Question 13

GIVE FOUR (4) types of clearing agents

Answer 13

CEDAR WOOD OIL,
BENZENE,
XYLENE,
CHLOROFORM

Question 14

Give SEVEN (7) types of reagents on the
tissue processor (Autotechnicon)

Answer 14

10% NEUTRAL BUFFERED FORMALIN
70% ETHANOL
80% ETHANOL
90% ETHANOL
100% ETHANOL
XYLENE
LIQUID PARAFFIN

Question 15

Give at least TWO (2) analytical process in Histopathology

Answer 15

GROSS EXAMINATION,
TISSUE FIXATION,
TISSUE PROCESSING