Gene Expression Measurement Methods

Question 1

A technique that permits visualization of molecules (mostly protein) in fixed biological samples (tissue or cell)

Answer 1

Immunoflorescence

Question 2

A type of immunofluorescence that uses a single, primary antibody, chemically linked to a fluorophore

Answer 2

Primary (Direct) Immunofluorescence

Question 3

A type of immunofluorescence that uses two antibodies: the unlabeled primary antibody which specifically binds the target molecule, and the secondary antibody which carries the fluorophore, recognizes the primary antibody, and binds to it

Answer 3

Secondary (Indirect) Immunofluorescence

Question 4

The protein of interest in immunofluorescence

Answer 4

Antigen

Question 5

In immunofluorescence, the part of the antigen recognized by the antibody

Answer 5

Epitope

Question 6

The identification of molecules of interest using specifically designed molecular probes, which bind to the target protein (by antibody) or DNA/RNA (by base pairing), and can be detected

Answer 6

Blotting

Question 7

Blotting to detect DNA

Answer 7

Southern Blot

Question 8

Blotting to detect RNA

Answer 8

Northern Blot

Question 9

Blotting to detect protein

Answer 9

Western Blot

Question 10

A technique for detecting mRNA in fixed tissues, to detect gene expression

Answer 10

In Situ Hybridization

Question 11

A technique for measuring the mRNA level for the gene of interest using PCR

Answer 11

qPCR

Question 12

A technique for analyzing the abundance of mRNA for all the genes in parallel

Answer 12

RNA Sequencing