Genomics and Transgenes

Question 1

Organisms vary in the degree to which certain degenerate codons are used, can affect transgene expression

Answer 1

Codon Bias

Question 2

Transgenes that can be used to identify regulatory sequences by carrying transcriptional or translation fusion to a reporter gene

Answer 2

Transgene Reporter Assays

Question 3

Attempts to understand the content, organization, function, and evolution of the genetic information contained in whole genomes

Answer 3

Genomics

Question 4

A sequencing method used in the human genome project in which the chromosomal location of each clone was identified using mapping

Answer 4

Clone (Map-Based) Sequencing

Question 5

A sequencing method used in the human genome project that went straight to shotgun sequencing without genetic mapping.

Answer 5

Whole Genome Sequencing (WGS)

Question 6

A technique for determining the DNA sequence of an organism’s genome by randomly breaking up the genome into many smaller DNA fragments that are sequenced individually. A computer program then looks for overlaps in the DNA sequences and uses them to reassemble the fragments in their correct order

Answer 6

Shotgun Sequencing

Question 7

The process of attaching biological functions to the DNA sequences, based on experimental evidence (preferably) or computational analysis

Answer 7

Annotation

Question 8

The process of identifying the location of genes and functional sequences within the genome sequence

Answer 8

Genome Annotation