Bacteriology - Mycology Intro

Question 1

Importance of fungi being eukaryotic

Answer 1

Key for drug use, eukaryotic cells of fungi are similar to host cells - drugs can attack both = toxic

Question 2

Ergosterol

Answer 2

Replaces cholesterol in fungi cells, what “azol” drug target

Question 3

Yeast formation

Answer 3

Unicellular fungus, spreads by budding

Question 4

Hyphae

Answer 4

Filamentous form of fungus

Question 5

Pseudohyphae

Answer 5

Chains of yeast could appear like hyphae

Question 6

Mold formation

Answer 6

Fungus that grows as a mass of hyphae (mycelium)

Question 7

Dermatophyte form

Answer 7

Fungus that causes ringworm (superficial infections of skin, hair, nails)

Question 8

Endospores

Answer 8

Spores contained in a sac

Question 9

Dimorphism fungus

Answer 9

Fungus that exists as yeast forms in animal tissue & as hyphae in environment

Question 10

Broad vs narrow based budding yeast

Answer 10

Broad - two spheres in one spore
Narrow - bowling pin shape

Question 11

Fruiting body

Answer 11

Produces many conidia, tiny & light for easy dispersion (like a dandylion)

Question 12

Arthroconidia

Answer 12

Spores released during hyphal fragmentation, occurs in patients

Question 13

Macroconidia

Answer 13

Growth of spores that occur in vitro

Question 14

Factors that effect development of fungal disease (5)

Answer 14

Virulence factors, tissues affected, host immunity, geographic location, environment

Question 15

Best diagnostic test for superficial fungi

Answer 15

Wet mount cytology
- 10% K hydroxide
- new meth blue

Question 16

Temps for sending out samples

Answer 16

Dermatophytes - room temp
Fungal disease - room temp
Bacterial contamination or differential diagnosis - on ice to stall bacterial growth

Question 17

In house Dermatophyte media

Answer 17

DTM - Dermatophyte test media
RSM - rapid sporulated media

Question 18

DTM

Answer 18

pH indicator, turns red as Dermatophyte grows and eats protein (increases pH) false positives if you wait too long