Ch 6-12 TEST 2 Flashcards
What is the preferred temperature range for a Psychrophile?
Psychrophiles 10C - 20C
What is the preferred temperature range for Psychotroph?
Psychotroph 0 to 20-30 C
What is the preferred temperature range for a Mesophiles?
Mesophiles 25 to 40 C
What PH range do most bacteria grown in?
PH 6.5 & 7.5
What PH range do most molds grow in?
PH 5 & 6
Explain the importance of osmotic pressure to microbial growth.
Since cells are made up of 80-90% water, if placed in a solution that is hypertonic (high salt/sugar), the cell will go under plasmolysis, which is osmotic loss of water from the cell. This would stop the growth of the microbe & vice versa in a hypotonic solution which could cause lysis.
What is carbon used for in microbial growth?
Energy source - they are structural organic molecules.
What microorganisms use organic carbon sources, & what are they?
Chemoherterotrophs - use carbohydrates, proteins & lipids.
What microorganisms use Carbon Dioxide for energy?
Chemoautotrophs - use CO2
What temperature is ideal for human pathogenic bacteria to grow & is ideal for a mesophilic?
37 C
What type of bacteria grows in an acidic environment?
Acidophiles
What makes up a Hypertonic Environment & what is it used for?
High salt & sugar concentrated - causes plasmolysis. It’s ued to preserve foods (honey, jams, meat)
What is a good example of a hypotonic solution?
Distilled water, water enters the cell & may cause lysis (cell wall to rupture) if it is weak.
Where is nitrogen located in a microbe?
In amino acids, DNA & RNA.
Where is Sulfur located in a microbe?
In amino acids, thiain & biotin.
Where is Phosphorus located in a microbe?
In DNA, RNA, ATP & membranes (phospholipids).
What could be a trace element in a microbe?
Usually as an enzyme cofactor they are: potassium, calcium, magnesium, iron, copper & zinc.
What is an obligate aerobe?
Requires oxygen
What is an facultative aerobe?
Grows with or w/o oxygen, but grows better w/ the oxygen present
What is an obligate anaerobe?
Cannot grow in presence of oxygen.
How do aerobes avoid damage by toxic forms of oxygen?
By using enzymes to neutralize toxic forms of oxygen. Superoxide free radical - dismutase. Peroxide anion - catalyse & peroxidase.
What is the formation of biofilms?
- Microbial Communities 2. Form slime/hydrogels 3. Attached to a surface 3.Share Nutrients
Why are biofilms potential causes for infection?
Because they work as a small community, they are shelted from harmful factors due to their slime layer. They are more resistant to microbes & they are prone to forming on indwelling medical devices.
What is chemically defined media?
Exact chemical composition is known
What is complex media?
Composition of nutrient varies: Composed of extracts of yeasts, meat or plants. *nutrient broth *nutrient agar
What is a culture medium?
Nutrient material prepared for microbial growth. Contains all nutrients required for microbial growth. Must be sterile (no living microbes)
Describe the characteristics of Agar.
- Complex polysaccharide from algea 2. used as solidifying agent for culture media in Petri Plates & Slants 3. Generally not metabolized by microbes. 4. Liquefies @ 100 C 5. Solidifies at -40C
Why do you use anaerobic technique?
to grow anaerobes
Why do you use Selective Media?
to suppress unwanted microbes
Why do you use Differential Media?
to distinguish between different microbes
Why do you use enrichment media?
to encourage growth of a microbe.
What is a pure culture?
contains only one species or strain
What is a colony?
Is a population of cells arising from a single cell, spore, or from a group of attached cells.
Why is a streak plate method used?
To isolate pure cultures.
What is the temp range for deep freezing?
-50C to -95C - it restricts growth
What is the temp & purpose of Lyophilization?
(freeze drying) -54 C to -72C - & it dehydrates the cells in a vacuum to restrict growth.
What are methods of reproduction of Bacteria?
- Binary fission - normal method. 2. Budding 3. Conidiospores 4. Fragmentation of filaments
What are the 4 phases of microbial growth?
- Lag phase (preparing for growth, but not production) 2. Log phase (binary fission, exponential growth) 3. Stationary Phase (equal growth & dying) 4. Dying phase (dying exponentially)
What are 4 methods of measuring cell growth?
- Serial dilutions 2. Pour Plate Method 3. Spread plate method 4. Filtration
What are direct methods for measuring cell growth?
- Plate counts 2. filtration 3. direct microscope count
What are indirect methods of measuring cell growth?
- Turbidity 2. Metabolic activity 3. Dry Weight
Sterilization
Removal of all microbial life
Commercial sterilization
Klling C. Boulinum endospores
Disinfection
Removal of pathogens (vegetative)
Antisepsis
Removal of pathogens from living tissue
Degerming
Mechanical removal of microbes from a limited area ( alcohol swab )
Sanitization
Lower microbial counts to safe public health levels & minimize disease transmission (eg eating utensils)
Biocide/Germicide
Treatments that kill microbes
Bacteriostasis
Treatments that inhibit, not kill, microbes.
Sepsis
refers to microbial contamintation
Asepsis
absence of significant contamination
Aseptic
an object or area is free of pathogens
Aseptic techniques
Techniques to minimize contamination (eg surgical instruments, operating personnel, patient)
At what rate do microbes die?
90%/ min
What are factors that influence the effectiveness of anitmicrobial?
- The # of microbes 2. Environmental Influences 3. Time of exposure 4. Microbial characteristics
What determines how well an antimicrobial treatment works?
The # of microbes influences the effectiveness of the antimicrobial treatment. ** The cells in a population die at a constant rate.
How do agents kill or inhibit microbes?
- Alteration of membrane permeability (damage lipids or protein components) 2. Damage Proteins 3. Damage to Nucleic acids (DNA & RNA).
What are types of physical methods of microbial control?
- Heat - Moist, pasteurization & dry heat sterilization 2. Filtration 3. Low Temperature 4. High Pressure 5. Desiccation 6. Osmotic Pressure 7.Radiation
Thermal death point (TDP) (from heat)
Lowest temperature at which all cells in a culture are killed in 10 min
Thermal death time (TDT) (from heat)
Time during which all cells in a culture are killed
Decimal Reduction time (DRT)
Minutes to kill 90% of a population at a given temperature
How does moist heat work?
It denaturation (coagulation) of proteins
Boiling
Does not kill endospores or some viruses
Autoclaving
Steam under pressure, temp. above boiling water (121 C) *sterilizes
Steam Sterilization
Steam MUST contact item’s surface
Pasteurization
Reduces spoilage organisms and pathogens; used for dairy products. Uses high temps @ short time *72C for 15 secs *Thermoduric organisms survive (usually not pathogenic)
Ultra high Temperature
sterilizes *heat to 140 C for 4 secs