Krebs cycle

Question 1

What does the citrate synthase do?

Answer 1

1.The citrate synthase enzyme fuses Acetyle coa and oxaloaceacetate together
2.If there is too much ATP , citrate, succonylcoa & NADH it will inhibit the enzyme to making it
3.ADP stimulates the enzyme making it go through the kreb cycle to produce more ATP.

Question 2

What happens in the aconitase?

Answer 2

1. The citrate to isocitrate and the still the same 6 in citrate and 6 in isocitrate but this a isomerisation reaction what is reverisable.

Question 3

What happen in the Isocitrate dehydrogenase?

Answer 3

1. NAD + into NADH
   2.Isocitrate goes to alpha kentogultarate D.H and it loses one carbon to CO2 through decarboxlycation
   3.There are binding site, ATP binding and inhibits it and Ca+ ions and ADP stimulate to make ATP.

Question 4

Alpha ketogulatarte D.H

Answer 4

1.Alpha kentogulatarate goes to Alpha kentogulatarate enzyme and it loses one carbon through decarboxylation to Co2 (Now 4 carbons)
2.NAD+ into NADH
3.Succinyl coa and NADH inhibits the enzymes to making stop production of it.
4. Calcium stimulates the enzyme to speed up ATP production

Question 5

Succinyl coa synthesase

Answer 5

1. CoA is added then removed
   2.GDP- GTP-ADP (gains phosphate)-ATP
   3.ATP blocks the GTP so the GTP-GDP
   4.Succinyl coa itno sucinate

Question 6

Succinate dh enzyme

Answer 6

1.FAD- FADH2
2.Reverisble reaction
3.Can lead to pheochromocytotoma which os a tumour in the adernal medulla

Question 7

Fumarase

Answer 7

1. H20 is added to when it goes from fumarase to malate
2. Deficinicy in this enzyme leads to a tumour leiomas- benign in smooth muscles

Question 8

Malate dehydrogenase?

Answer 8

1. NAD- NADH
2. Reversible reaction