13 - Hfr konjugacija Flashcards

1
Q

ali se lahko plazmid vključi v kromosom

A

da
če ima mesta homologije s kromosomom se lahko s homologno rekombinacijo vključi v kromosom

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2
Q

kako se vstavi

A

s homologno rekombinacijo

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3
Q

kaj so mesta homologije

A

transpozicijski elementi:
transpozoni in insercijska zaporedja

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4
Q

integriran plazmid je ___

A

episom

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5
Q

ali se lahko vstavljen plazmid izreže iz kromosoma

A

da
Hfr —> izključitev —> F’ (F prime) — če pride do nenatančnega izrezovanja (npr lac ostane na plazidu in ne gre na kromosom) oz F+ če je natančno izrezovanje

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6
Q

nastanek diploidne celice

A

če imamo F’ celico (ta ima F plazmid za lac +in kromosom) in poteče konjuacija z F- (ima lac-) bosta nastali dve celici: F’lac+ in F’lac+lac- (MEROZIGOTNA CELICA)

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7
Q

MEROZIGOTNA CELICA

A

prokariont diploiden za določen alel

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8
Q

ENDOGENOT

A

recipientska DNA

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9
Q

EKSOGENOT

A

tuja DNA

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10
Q

uporaba Hfr sevov

A

za mapiranje kromosomov

večja je frekvenca prenosa, bližje je gen začetku prenosa.

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