Things I forget

Question 1

How do you rehydrate your slides for staining?

Answer 1

6 minutes for Paraclear 1
5 minutes for Paraclear 2
4 minutes for IMS 1
3 minutes IMS 2
2 minutes for Spirit
1 minute for Water

Question 2

How do you dehydrate your slides for cover-slipping?

Answer 2

Dab off as much water as possible -> shake off
10 seconds spirit (if you’ve blotted you only need to dip)
1 minute IMS1
3 minutes IMS2
3 minutes in paraclear 1
3+ minutes in paraclear 2

Question 3

How do you rapid dehydrate your slide?

Answer 3

Ask for filter paper
Blot dry
Go straight to IMS 1 minute, IMS2, Paraclear 1, Paraclear 2

Question 4

What are the two types of silver stains and give examples?

Answer 4

argyrophilic = Gordon and Sweets
argentaffin = Grocotts

Question 5

What is a regressive and what is a progressive stain?

Answer 5

Regressive = needs differentiation /it stains everything = haematoxylin

Progressive = no need for differentiation / usually a counterstain = eosin

Question 6

What type of haematoxylin us used in H&E

Answer 6

Aluminium mordanted haematoxylin