NSCs and Adult Neurogenesis

Question 1

totipotent vs pluripotent vs multipotent

Answer 1

totipotent - generate every cell type in embryo and placenta

pluripotent - generate every cell type in embryo

multipotent - generate multiple specialised cell types in a tissue

Question 2

population studies of stem cells

Answer 2

immunohistochemisty
single cell RNA-sequencing
thymidine analogues

Question 3

clonal analysis

Answer 3

lineage tracing

genetic or viral mediated labelling most common

Question 4

neurogenesis in SVZ

Answer 4

NSCs are Type B cells. They generate Type C cells which are neural progenitor cells. TC generate TA cells which are neuroblasts, that migrate through RMS to olfactory bulb

differentiate into different types of interneurons

Question 5

neurogenesis in hippocampus

Answer 5

dentate gyrus
NSCs are radial glial-like cells, multipotent and usually quiescent
only granule cells are generated