Approaches to sutding the NS (anatomy) Flashcards
Electrophysiology summary:
- Extracellular: CAP, EEG
- Intracellular microelectrodes
- Voltage clamp
- Current clamp
- Patch clamp
Types of imaging summary:
- Microscopy
- Immunohistochemistry
- MRI
- fMRI
- PET
- CT
Optical imaging summary:
- Voltage sensitive dyes
- Optogenics
- Ca2+ sensitive dyes
Animal behavioural observation summary:
- Morris water maze
- Novel object recognition
- Elevated plus maze
- Foot shock
Molecular biology summary:
- PCR
- Gel electerophoresis & blotting
- ELISA
- In situ hybridisation
Model systems summary:
- Animal
- In vitro (cell culture)
- Computational
Experimental manipulations summary:
- Surgival
- Pharmacological
- Genetic
Where did electrophysiology start?
- 1780s –> Galvani & Galeazzi, stimulated frog spinal cord causing the legs to move
What were the techniques for electrophysiology that took off in the 20th century?
- Erlanger & Gasser, CAP
- Hodkin & Huxley, intracellular electrophysiology
- Hodkin & Huxley, voltage clamp
- Gerad, Graham & Ling, intracellular microelectrodes
- Neher & Sakmann, patch clamp
What does the CAP stand for in extracellular electrophysiology: CAP?
Compound action potential
What is Compound action potential (CAP)?
Population response, some of APs from all the axons simultaneously contributing to the signal
What do extracellular electrodes measure?
Compound action potential
What do tracers measure?
Neuronal connections
What do voltage clamps measure?
Whole cell current
What does single channel recording measure?
Currents that flow through ion channels
