rct irrigation Flashcards

1
Q

What is responsible for endo infection?

A

Obligate anaerobic bacteria (polymicrobial)

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2
Q

What are the aims of irrigation?

A

Kill and remove microorganisms
Remove necrotic/inflamed tissue, dentine debris and smear layer
Provides lubrication and reduces friction for instrumentation

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3
Q

What are the obstacles in disinfection?

A

1. Anatomical complexities- only 61% instrumented, rest remain infected
2. Smear layer- protection for bacteria, inhibits/delays irrigant into tubules
3. Fluid dynamics
4. Biofilm and resistant microbiota

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4
Q

How are there anatomical complexities?

A

Webs
Loops
Cul de sac
Fins
Lateral canals
Apical delta

Types I-V

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5
Q

What does the smear layer consist of?

A

In/organic substances
Fragments of odontoblastic processes
Microorganisms
Necrotic debris

Created during cleaning and shaping

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6
Q

What are fluid dynamics?

A

Irrigant only penetrates 1-2mm around needle
Absence of fluid motion- stagnation plane and no flushing of debris in apical region

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7
Q

What are fluid dynamic rules for irrigation?

A

1. Side venting needle
2. Luer-lok syringe
3. Keep needle tip moving
4. Gentle pressure
5. If needle binds- stop
6. Constant agitation to improve flow
7. Size 27/30 gauge needle

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8
Q

How far should the needle be from the apex?

A

2mm

3-4mm if open apex

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9
Q

What is dynamic pumping?

A

Introduce gutta percha point matched to master cone in a flooded canal to working length

Gentle in and out motion to stir up irrigant

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10
Q

What is a biofilm?

A

Any group of microorganisms in which cells stick to each other on a surface
Frequently embedded in EPS (extracellular polymeric substance)

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11
Q

What is EPS?

A

Polymeric conglomeration of extracellular DNA, proteins and polysaccharides

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12
Q

What are planktonic cells?

A

Single cells that may float in a liquid medium
- easily flushed out

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13
Q

What is MIC?

A

Minimum inhibitory concentration
Can be 100-1000x higher in biofilm than suspension culture

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14
Q

What is an example of a resistant bacteria?

A

E. faecalis
Can survive as monoculture in canals
Most freq species in root tx teeth w prevalence value of 90%
High resistance to NaOCl

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15
Q

What are ideal characteristics of an endo irrigant?

A

1. Effective antimicrobial
2. Non-irritating
3. Stable in solution
4. Prolonged effect
5. Active in blood, serum and protein derivatives of tissue
6. Low surface tension
7. Not interfere w repair
8. Non staining
9. Doesn’t induce cell mediate I. response
10. Completely remove smear layer
11. Non toxic/antigenic/carcinogenic
12. No adverse effects on physical properties of exposed dentine
13. No adverse effects on sealing ability of materials
14. Easy to use
15. Inexpensive

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16
Q

What are current irrigant solutions?

A

1. NaOCl
2. EDTA
3. CHX
4. IPI
5. MTAD
6. Hydrogen peroxide
7. PAD
8. Electronically activated water

17
Q

What is NaOCl?

A

Sodium hypochlorite- essentially supermarket bleach w/o thickener

1. Only irrigant that dissolves organic inc. biofilm
2. Effective against all endo bacteria
3. Affordable

0.5%- 30 min
2.5%- 10 min
4%- 5 min
5.25%- <30s

18
Q

What happens if you increase the temp of NaOCl?

A

1. Improve tissue dissolution (0.5% at 45C=5.25%)
2. 60C completely dissolve tissue but destroy osteoblasts and cause metal corrosion
3. Increase antimicrobial effect (2x each 5C rise)

19
Q

Why do you need to continuously replenish NaOCl?

A

Chloride ion responsible is rapidly consumed (2min) esp. in first phase

20
Q

What are disadvantages of NaOCl?

A

1. Minimal removal of dentine debris/smear layer
2. Reduces flexural strength
3. Rare allergy
4. Toxic to vital tissue (haemolysis, epithelial ulceration, necrosis)
5. Corrosion to metal

21
Q

What is EDTA?

A

Ethylenediamine tetra-acetic acid
Solution- 15-17% (penultimate rinse for 1-2mins)
Gel form- glyde- usually for sclerosed, aids lubrication
Synthetic AA
Removes smear layer as it’s a ‘chelating agent’
Not bactericidal/static (eventually kills by starvation due to chelation)
Relatively non toxic
Shouldn’t over use- excessive removal of dentine

22
Q

What is a chelating agent?

A

Scavenges up and forms ring shaped internal complexes w metallic ions, inc. Calcium

23
Q

Is citric acid used?

A

Not as effective chelating agent than EDTA

24
Q

What is chlorhexidine?

A

Cationic bisbiguanide antiseptic
Broad spectrum
Bacteriacidal at high conc
Static at low conc
Substantivity
Conc- 0.2-2%
Less toxic than NaOCl
No harmful effects to dentine

25
Q

What are disadvantages of CHX?

A

1. Forms precipitate w NaOCl- para chloroanaline (brown, potentially carcinogenic)- use intermediate as saline before using
2. No tissue dissolving effects
3. Can’t remove smear layer
4. Severe allergic reaction rare

26
Q

What is IPI?

A

Iodine (2%) potassium iodide (4%)
Acts as oxidising agent attacking key group proteins, nucleotides and FAs
Broad spectrum inc. E faecalis
Substantive
Low toxicity

27
Q

What are disadvantages of IPI?

A

1. Allergic reaction to iodine
2. Stains dentine
3. Not stable w organic
4. Doesn’t dissolve tissue/remove smear layer

28
Q

What is hydrogen peroxide?

A

Hydroxy free radicals destroy proteins and DNA
Conc- 3-5%
Active against bacteria, virus, yeast
Not used routinely anymore

29
Q

What are disadvantages of hydrogen peroxide?

A

1. Poor antimicrobial efficacy
2. Low tissue dissolving
3. Can release O2 gas (seepage in tissue may lead to air emphysema)

30
Q

What is MTAD?

A

Mixture of-
3% doxycycline (broad spectrum)
4.25% citric acid (removes smear layer)
Surface active detergent

No adverse effect on physical properties

31
Q

What are disadvantages of MTAD?

A

1. Less efficient on biofilm
2. Toxic
3. Expensive
4. Issues w bacterial resistance
5. Risk of tetracycline staining

32
Q

What is PAD?

A

Photo activated disinfection
Toluidine blue dye
Low power laser activated
Photosensitiser molecules attach to bacteria and singlet oxygen produced to rupture cell wall
Broad spectrum
No toxicity

33
Q

What are disadvantages of PAD?

A

1. Depends on power, length of exposure, absorption of light and distance
2. No benefit over others
3. Expensive

34
Q

What is electronically activated water?

A

Electrolyses saline solution
Creates hypochlorous acid and free radicals to remove biofilm and destroy bacteria
Lack studies showing efficacy/safety

35
Q

What is ultrasonic agitation of irrigants?

A

Acoustic micro steaming
Enhances antibacterial effect of irrigants
Disrupts biofilm
Eliminates more bacteria
Can use specific tips/standard files