Sterilization, Disinfection, and Antisepsis Flashcards

1
Q

sterilization

A
  • describes a process that destroys or eliminates all forms of microbial life and is carried out in health care facilities by physical or chemical methods
  • reduces numbers of microorganisms on a device to a level that is insufficient to transmit infectious organisms
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2
Q

disinfection

A
  • describes a process that eliminates many or all pathogenic microorganisms, except bacterial spores, on inanimate objects
  • doesn’t necessarily kill all microorganisms
  • reduces numbers of microorganisms on a device to a level that is insufficient to transmit infectious organisms
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3
Q

Health care associated infections

A
  • over a quarter of all hospital acquired HAIs are caused by four types of infections:
  • pneumonia
  • bloodstream infection
  • urinary tract infection
  • surgical site infection
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4
Q

high level disinfection

A
  • destroys all microorganisms except high number of bacterial spores
  • pasteurization and liquid immersion in chemical sterilants (used for heat sensitive semi-critical items such as GI endoscopes, bronchoscopes
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5
Q

intermediate level disinfection

A
  • destroys vegetative bactera, mycobacteria, most viruses, most fungi, not bacterial spores
  • hospital disinfectants with label claim regarding tuberculocidal activity used in non critical patient care items or surfaces with visible blood
  • chlorine based products
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6
Q

low level disinfection

A
  • destroys vegetative bacteria, some fungi and viruses, not sports or mycobacteria
  • no tuberculocidal claim-phenolics, quants, 70-90% alcohol
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7
Q

antisepsis

A
  • the reduction of microorganisms on living skin/tissue

- do not kill spores and can’t be used as disinfectants

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8
Q

HAI risk factors

A
  • use of indwelling medical devices such as bloodstream, endotracheal, and urinary catheters
  • transmission of communicable diseases between patients and HCWs
  • surgical procedures
  • injections
  • contamination of healthcare environment
  • overuse or improper use of antibiotics
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9
Q

challenges to avoiding HAIs

A
  • reprocessing of medical devices is a challenge w
  • endoscopes are probably the most challenging reprocessing task in health care
  • flexible endoscopes have intricate sophisticated small parts that are difficult to clean before they can be disinfected
  • 23.0% of 71 internal channels grew more than 10^6 CFU and 78% of facilities didn’t sterilize forceps
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10
Q

Ignaz Semmelweis

A
  • professor of obsetrics
  • thought particles from cadavers were spreading disease
  • said they should soak hands in chlorine (1847)
  • people called him crazy
  • died in mental institution
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11
Q

Florence Nightingal

A

-showed mortality rate in most hospitals was over 50%

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12
Q

Joseph Lister

A
  • 1865 Pasteur said decay was caused by organisms in air that cause fermentation-lister made connection with wound sepsis
  • used carbolic acid to sterilize wounds
  • ward remained clear of sepsis for 9 months
  • 1869-spray techniques for aseptic surgery
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13
Q

decontamination

A
  • removal of debris, blood, and proteins and most microorganisms
  • not necessarily rendering device safe to handle by HCWs who are not wearing protective attire
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14
Q

critical items

A
  • instruments or object that are introduced directly into the bloodstream of into other normally sterile areas of the body
  • want sterilization
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15
Q

semicritical items

A
  • non-invasive flexible and rigid fiber optic endoscopes, endotracheal tubes, anesthesia breathing circuits and cytoscopes
  • come into contact with mucous membranes or non intact skin, should be free of everything except spores
  • high level disinfectant
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16
Q

non-critical items

A
  • those that either do not ordinarily touch the patient and touch only in tact skin
  • have to be careful about MRSA
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17
Q

cidal

A
  • killing of microoganism
  • germicides
  • kill microbes but not necessarily their endospores
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18
Q

static/stasis

A

-inhibit growth or multiplication of microorganism

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19
Q

Resistance

A
  • there is a scale of how resistant bacteria and viruses are to various disinfectants
  • lipid enveloped viruses most susceptible, prions most resistant
20
Q

conditions influencing the effectiveness of physical and chemical microbial agens

A
  • population size
  • type of microbe
  • physiological state of microbe
  • concentration or intensity of antimicrobial agent
  • duration of exposure
  • temperature
  • environmental factors
  • amount of organic contamination
  • pH
21
Q

physical methods of microbial control

A
  • heat-incineration, dry heat, moist heat (below 100-pasteurization, 100=boiling, above 100=autoclave
  • radiation-UV, ionizing
  • Filtration
22
Q

autoclaving

A
  • steam under pressure
  • 121 C/15 psi for 15-90 min
  • 132 C /27 psi for 4-20 min
  • minimal time required
  • loading and packing critical to performance
  • non toxic to patient staff and enviro
  • rapidly microbicidal
  • penetrates medical packaging, device lumens
  • can’t use for heat sensitive instruments
  • microsurgical instruments damaged by repeated exposure
23
Q

flash sterilization

A
  • process of sterilizing unwrapped instruments
  • uses steam for 3 min at 270 F (132 C) at 27-28 psi
  • not routine, only OR
24
Q

pasteurization

A

does not achieve sterility but kills pathogens that might be present in milk, dairy products, beer-mycobacterium, salmonella

  • classic-63 C 30 min
  • flash-high temp short time (HTST) 72 C 15 sec rapid cooling
  • ultra high treatments (UHT) 140 C less than 1 sec, rapid cooling
25
Q

filtration

A
  • excellent way to sterilize solution of heat sensitive materials
  • membrane filters of .1 mm thickness-cellulose acetate, cellulose nitrate, polycarbonate, polyvinylidene, difluoride
  • pore size .45 or .22 mm for bacteria
  • .01 mm for spirochetes, mycoplasma, viruses, and large proteins
26
Q

masks

A
  • physical barrier between your mouth and nose and environment
  • N95 are 95% efficient at filtering out particles that are greater than 3 micrometers in diameter
  • droplets emitted by sneezing, coughing, talking are 5 micrometers or bigger, so these work well for flu or TB
  • must be fit properly every time they are used
27
Q

ionizing radiation

A
  • gamma, x, high E electron beams
  • ionization of water forms highly reactive hydroxyl radicals
  • uses-pharmaceuticals, disposable dental and medical supplies (syringes, gloves, sutures)
28
Q

nonionizing radiation

A
  • UV-thymine dimers inhibit replication of DNA
  • not penetrating
  • damages eyes, skin cancer, burns
29
Q

irradiated blood products

A
  • irradiated RBCs help to prevent graft versus host disease

- prevents proliferation of leukocytes and T cells so the graft can’t attack the host

30
Q

irradiated food

A
  • destroys bacteria by damaging DNA
  • prolongs shelf life of food in cases where microbial spoilage is the limiting factor
  • treatment of hamburger eliminates the residual risk of a contamination by e coli
31
Q

phenol and phenolics

A
  • exert antimicrobial activity by injuring PM, inactivate enzymes and denature proteins
  • carbolic acid irritates skin and smell
  • used to intermediate and low level disinfection
  • triclosan-only good for gingivitis and may be contributing to antibiotic resistance
32
Q

hexachlorophene (pHisoHex)

A
  • most active against gram pos bacteria (staph aureus)
  • toxic in newborns
  • now used for staph skin decolonization in patients undergoing prosthetic or cardiac valve implant surgery
33
Q

chlohexidine

A
  • not a phenol deriv
  • frequently used for disinfection of skin and mucous membranes
  • chloraprep
  • used for surgical hand scrubs and preop skin prep
  • low toxicity, strong affinity to skin or mucous membranes
  • killing effect by damaging PM
  • effective against most vegetative bacteria but not sporicidal
  • can be used in oral rinses
  • some people allergic, some conferring resistance
34
Q

aldehydes

A
  • work by forming covalent crosslinks with a number of organic functional groups on proteins
  • formalin-37% aq solution of formaldehyde gas
  • glutaraldehyde-used to sterilize hospital instruments
  • less irritating and more effective that formaldehyde
  • Cidex-bacteriocidal,tuberculocida, virucidal in 10 min, sporucidal in 3-10 hours
  • only liquid disinfectant that can be considered a sterilant
35
Q

gaseous chemosterilizers

A
  • ethylene oxide, propylene oxide
  • denaturation of proteins by alkylation
  • sterilize without heat
  • sterilization of medical supplies and equipment:disposable plastic syringes, lensed instruments, artificial heart valves, heart lung machine, mattresses
  • formaldehyde gas rarely used
  • ethylene oxide requires aeration time to remove ETO residue- toxic, carcinogen, flammable
36
Q

formaldehyde gas

A
  • AD-same as formalin, effective penetration
  • DISAD-same as formalin, flammable, pot carcinogen, gas irritates
  • uses-on site decontamination of biological safety cabinet, HEPA filters
  • enclosed areas
37
Q

ETO has

A
  • AD-broad spectrum, no heat/moisture, penetrates packing
  • DISAD-flammable, reactive, carcinogen/mutagen, some items may need more than 24 hrs for outgassing
  • uses:heat or moisture sensitive supplies, instruments, equipment
38
Q

Quaternary Ammonium Compounds

A
  • Ad-combined detergent and germicidal activity, stable and working dilutions have low toxicity
  • Disad-non-sporicidal, limited activity against viruses, mycobacteria, may support growth of bacteria
  • uses-surface decontamination, floors, walls, equipment wipe down, antiseptic formulations available
  • crestm cepacol
  • can be resistant
39
Q

alcohols

A
  • dehydrate cells, disrupt membranes and caus coagulation of protein
  • may be used for hard surface disinfection of skin antisepsis
  • isopropyl, methyl, and ethyl
  • a 70% aqueous solution is more effective at killing microbes than absolute alcohols
  • skin disinfectant, surface decontamination, bench top/cabinet wipedown
  • inactivates most viruses with lipid envelope
  • can’t penetrate protein rich materials
  • flammable
40
Q

handwashing and sanitizer

A
  • less time, more effective unless hands are visibly soiled
  • reduces bacterial counts on hands
  • more accessible than sinks
  • physicians more willing than washing hands
41
Q

halogens-iodine and chlorine

A
  • oxidizing agents and cause damage by oxidation of essential sulfydryl groups of enzymes
  • chlorine reacts with water to form hypochlorous acid, which is microbicidal
  • chlorine gas is used to bleach water
  • household bleach can be used at a dilution of 1:10 in decontamination of spillage of infectious material
  • disadvantage-unstable when diluted-needs to be made daily, don’t work on organic matter as well, may be corrosive
42
Q

iodine preparations

A
  • tincture of iodine (2% iodine, 70% alcohol) is an antiseptic
  • iodophores-iodine can be combined with neutral carriers such as polyvinylpyrrolidone to prepare iodophores such as povidone-iodine
  • betadine,isodine
  • iodophores permit slow release and reduce irritation of antiseptic
43
Q

heavy metals and organic acids

A
  • metals combine with SH groups on proteins
  • heavy metals-silver nitrate (1% for ohpthalmic prep), mercurochrome, copper sulfate, zinc chloride/oxide
  • organic acid deriv-sorbic acid, benzoic acid, methylparaben, propylparaben
44
Q

h2o2

A
  • acts on the microorganisms through its release od nascent oxygen, produces hydroxyl free radicals that damages proteins and DNA
  • used at 6% concentration to decontaminate instruments and equipment such as ventilators
  • 3% used for skin disinfection and cleaning of wounds and ulcers
  • strong solutions are sporicidal
  • decomposes in light, broken down by catalase, proteinaceous organic matter drastically reduces activity
45
Q

Peracetic Acid

A
  • mixture of acetic acid and h2o2 in aq soln
  • PAA disinfects by oxidizing the outer cell membrane of vegetative bacterial cells, endospores, yeast, and mold spores
  • cooling tower water disinfectant where it prevents biofilm/legionella
  • effective for sanitizing fowl carcasses without affecting skin or flesh-applied to meat
  • plasma (gas phase) sterilization
46
Q

plasma sterilization

A
  • beneficial for sterilizing temperature sensitive polymeric material (endoscopes)
  • safe, non tixic dry, low temp sterilization in 1 hour
  • generated in an enclosed chamber under deep vacuum using UV or microwaves to create free radicals in a gaseous phase
  • free radicals kill bacteria
47
Q

STERRAD

A
  • uses low temp h2o2 gas plasma tech to sterilize instruments and medical devices
  • quick reprocessing
  • express cycle for da Vinvci 3D endoscopes and other devices without lumens
  • processes a wide range of instruments including multiple single channel flexible endoscopes, cameras, rigid scopes, light cords, batteries, power drills