Histopath-Lec-Cytology

Question 1

-microscopic examination of cells from different body sites for diagnostic purposes

Answer 1

Diagnostic Cytology

Question 2

2 Divisions of Cytology:

Answer 2

1. Exfoliative Cytology
2. Fine-needle Aspiration

Question 3

-microscopic examination of the shed, desquamated cells from the body surfaces of cells harvested by (1. rubbing, 2. brushing) epithelial surface

Answer 3

Exfoliative Cytology

Question 4

-procedure whereby a thin needle is inserted into the area of abnormally
appearing tissue or body fluid

Answer 4

Fine-needle Aspiration

Question 5

cells that are shed or remove from the epithelium

Answer 5

DESQUAMATED CELLS:

Question 6

GOALS of Exfoliative Cytology:

Answer 6

To detect malignancy or cancerous conditions
To detect asymptomatic cancer
To evaluate female hormonal activity
To determine genetic sex
To detect possible infection related to reproductive system

Question 7

Study of cells taken from the surface of excise or incise specimen by touching them from the slides

Answer 7

Imprint/Abraded Cytology

Question 8

Same with touch preparation

Answer 8

Imprint/Abraded Cytology

Question 9

Smear Technique Reminder: Smears are usually made form?

Answer 9

fresh materials

Question 10

Smear Technique Reminder: Smears must be prepared & immerse immediately in a _____ while still ______

Answer 10

fixation; moist

Question 11

Smear Technique Reminder: Fluid specimen must be centrifuge?

Answer 11

(2000 rpm) for 2 minutes

Question 12

Smear Technique Reminder: Extra sediments are used for?

Answer 12

cell block

Question 13

If smears cannot be made immediately:
Place in ______
Saccomano preservative: ______

Answer 13

50% alcohol, 95% ethanol
50% alcohol + 2% carbowax

Question 14

Fixatives for Cytology:

Answer 14

Equal parts of 95% ethyl alcohol and ether
95% ethyl alcohol
Carnoy’s fluid
Cytospray/spraycyte

Question 15

SMEAR PREPARATIONS:

Answer 15

streaking
spreading
pull-apart
touch preparation

Question 16

zigzag line

Answer 16

streaking

Question 17

teasing using applicator stick

Answer 17

spreading

Question 18

specimen spread evenly on two slides

Answer 18

pull-apart

Question 19

the slide is made to touch the cut surface of a tissue

Answer 19

touch preparation

Question 20

Specimen that requires ADHESIVE AGENT

Answer 20

Concentrated sputum
Urinary Sediment
Bronchial Lavage Specimen
Enzymatic Lavage

Question 21

ADHESIVES: (4)

Answer 21

Pooled human sera or plasma
Celloidin Ether-Alcohol
Leuconostoc culture
APES (3-aminopropyltriethoxysilane)

Question 22

***A good adhesive must be permeable to both?

Answer 22

fixative and stain

Question 23

Cell Block Technique: Also considered as

Answer 23

Microbiopsy

Question 24

Also considered as MICROBIOPSY

Answer 24

Cell Block Technique

Question 25

Uses of cell block: (4)

Answer 25

Architectural evaluation
Categorization of tumor
For Special stain and histochemistry
Archival material for future studies

Question 26

Centrifuge the fluid, the obtain sediment and add 10% formalin
Disadvantage: easily washed out

Answer 26

Direct Filtration

Question 27

Centrifuge the fluid, the obtain sediment, add 1mL plasma and add thrombin
Place filter paper then add 10% formalin

Answer 27

Plasma Thrombin

Question 28

Centrifuge the fluid, the obtain sediment & add carbowax; place in paraffin block

Answer 28

Carbowax method

Question 29

Technique collecting cells using filter paper with specified pore size

Answer 29

MEMBRANE FILTER METHOD

Question 30

MEMBRANE FILTER METHOD: The filter is made up of?

Answer 30

Polycarbonate and cellulose
ester

Question 31

Technique that allows cells to be spread on monolayer

Answer 31

LIQUID BASED CYTOLOGY