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BACTERIOLOGY > GRAM POSITIVE COCCUS > Flashcards

GRAM POSITIVE COCCUS Flashcards

1
Q

Differentiates members of family
Micrococcaceae/Staphylococcus from Streptococcus

A

CATALASE PEROXIDASE

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2
Q

Catalase Peroxidase PRINCIPLE : H20 + 02 : POSITIVE

A

bubbles or effervescent

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3
Q

Micrococcaceae and Staphylococci differentiation

A

Oxidative/ Fermentative : Glucose degradation

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4
Q

oxidative or inert (asaccharolytic)

A

Micrococci

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5
Q

Fermentative

A

Staphylococci

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6
Q

A positive carbohydrate utilization test is indicated by the development of a_____ color in the medium.

A

yellow

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7
Q

Development of a yellow coloration in the open tube only.

A

Oxidative

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8
Q

Development of a yellow coloration in both open and closed tubes.

A

Fermentative

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9
Q

A negative carbohydrate utilization test is indicated by the absence of a____ color media remains green or turns
_____.

A

yellow, blue

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10
Q

positive in catalase test

A

Staphylococcus and micrococci

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11
Q

Aerotolerant: Facultative anaerobe

A

Staphylococcus

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12
Q

catalase and modified oxidase positive

A

Micrococcus

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13
Q

Bacitracin resistant 0.04 u

A

Staphylococcus

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14
Q

Furazolidone and Lysostaphin Resistant

A

Micrococcus

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15
Q

Positive (+) cocci, spherical cells

A

GENUS STAPHYLOCOCCUS

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16
Q

“staphyle” meaning

A

bunch of grapes; arranged in clusters; but are also seen as single coccus, pairs, tetrads and chains.

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17
Q

Grow best in aerobic conditions but behave as facultative anaerobes

A

Catalase POSITIVE (+): Staphylococcus

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18
Q

Staphylococcus is :

A

non – motile; non – sporeforming

19
Q

Staphylococcal species:

A

Staphylococcus aureus
Staphylococcus epidermidis
Staphylococcus saprophyticus
Staphylococcus haemolyticus
Staphylococcus lugdunensis

20
Q

Chief Source of Infection of Staphylococcus :

A
  1. shedding from human lesions
  2. fomites
  3. contaminated lesion esp. respiratory tract and skin
  4. asymptomatic carriers among hospital staff & patient
21
Q

Mode of Transmission

A
  1. spread of patient’s endogenous strain to normally sterile site by traumatic induction (e.g.surgical/microabrasions) or as a result of implantation of medical devices (e.g. shunts, prosthetic devices)
  2. person to person transmission by fomites, etc
  3. transmitted from infected skin lesion
22
Q

MOST virulent species of Staphylococci
encountered

A

Staphylococcus aureus

23
Q

Staphylococcus aureus is responsible for _______in human.

A

80% of suppurative infection

24
Q

Capacity to produce disease not diminished even with the introduction of antibiotics.

A

Staphylococcus aureus

25
Q

important pathogen for hospital and community acquired infection

A

Staphylococcus aureus

26
Q

Dominant site of Colonization for S. aureus:

A

anterior nares
axilla
perineum
10-15% human skin

27
Q

Principal virulence of S. aureus:

A

coagulase

28
Q

S. aureus produce_____ or golden – yellow in BAP

A

lipochrome

29
Q

Beta-hemolytic

A

S. aureus

30
Q

3 Major Components of Cell Wall Structure of S. aureus.

A
  1. PEPTIDOGLYCAN
  2. TEICHOIC ACID
  3. PROTEIN A
31
Q

polysaccharide polymer which provide rigid exoskeleton of the cell wall; constitute 40 – 60% of the cell weight

A

PEPTIDOGLYCAN

32
Q

Elicits production of interleukin-1 and
opsonic antibodies by monocytes

A

PEPTIDOGLYCAN

33
Q

it can be a chemoattractant, have
endotoxin-like activity, and activate
complement.

A

PEPTIDOGLYCAN

34
Q

composed of acetylglucosamine and
acetylmuramic acid

A

PEPTIDOGLYCAN

35
Q

polymers of glycerol phosphate (S.
epidermidis) or ribitol phosphate (S. aureus)

A

TEICHOIC ACID

36
Q

plays important role in maintenance of
normal physiologic function of the cell

A

TEICHOIC ACID

37
Q

regulate the cationic environment of cell
thus control the activity of autolytic
enzyme responsible for growth of cell
wall.

A

TEICHOIC ACID

38
Q

acid antibodies detectable by
gel diffusion method

A

Antiteichoic

39
Q

a bacterial surface protein that has been
characterized among a group of adhesins
called microbial surface components
recognizing adhesive matrix molecules
(MSCRAMMS).

A

PROTEIN A

40
Q

(MSCRAMMS).

A

microbial surface components
recognizing adhesive matrix molecules

41
Q

major protein component of cell wall

A

PROTEIN A

42
Q

major antigenic determinant unique to
Staphylococcus aureus

A

PROTEIN A

43
Q

antiphagocytic (through binding to the Fc
portion of IgG)

A

PROTEIN A

BACTERIOLOGY (5 decks)

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