GRAM POSITIVE COCCUS

Question 1

Differentiates members of family
Micrococcaceae/Staphylococcus from Streptococcus

Answer 1

CATALASE PEROXIDASE

Question 2

Catalase Peroxidase PRINCIPLE : H20 + 02 : POSITIVE

Answer 2

bubbles or effervescent

Question 3

Micrococcaceae and Staphylococci differentiation

Answer 3

Oxidative/ Fermentative : Glucose degradation

Question 4

oxidative or inert (asaccharolytic)

Answer 4

Micrococci

Question 5

Fermentative

Answer 5

Staphylococci

Question 6

A positive carbohydrate utilization test is indicated by the development of a_____ color in the medium.

Answer 6

yellow

Question 7

Development of a yellow coloration in the open tube only.

Answer 7

Oxidative

Question 8

Development of a yellow coloration in both open and closed tubes.

Answer 8

Fermentative

Question 9

A negative carbohydrate utilization test is indicated by the absence of a____ color media remains green or turns
_____.

Answer 9

yellow, blue

Question 10

positive in catalase test

Answer 10

Staphylococcus and micrococci

Question 11

Aerotolerant: Facultative anaerobe

Answer 11

Staphylococcus

Question 12

catalase and modified oxidase positive

Answer 12

Micrococcus

Question 13

Bacitracin resistant 0.04 u

Answer 13

Staphylococcus

Question 14

Furazolidone and Lysostaphin Resistant

Answer 14

Micrococcus

Question 15

Positive (+) cocci, spherical cells

Answer 15

GENUS STAPHYLOCOCCUS

Question 16

“staphyle” meaning

Answer 16

bunch of grapes; arranged in clusters; but are also seen as single coccus, pairs, tetrads and chains.

Question 17

Grow best in aerobic conditions but behave as facultative anaerobes

Answer 17

Catalase POSITIVE (+): Staphylococcus

Question 18

Staphylococcus is :

Answer 18

non – motile; non – sporeforming

Question 19

Staphylococcal species:

Answer 19

Staphylococcus aureus
Staphylococcus epidermidis
Staphylococcus saprophyticus
Staphylococcus haemolyticus
Staphylococcus lugdunensis

Question 20

Chief Source of Infection of Staphylococcus :

Answer 20

1. shedding from human lesions
2. fomites
3. contaminated lesion esp. respiratory tract and skin
4. asymptomatic carriers among hospital staff & patient

Question 21

Mode of Transmission

Answer 21

1. spread of patient’s endogenous strain to normally sterile site by traumatic induction (e.g.surgical/microabrasions) or as a result of implantation of medical devices (e.g. shunts, prosthetic devices)
2. person to person transmission by fomites, etc
3. transmitted from infected skin lesion

Question 22

MOST virulent species of Staphylococci
encountered

Answer 22

Staphylococcus aureus

Question 23

Staphylococcus aureus is responsible for _______in human.

Answer 23

80% of suppurative infection

Question 24

Capacity to produce disease not diminished even with the introduction of antibiotics.

Answer 24

Staphylococcus aureus

Question 25

important pathogen for hospital and community acquired infection

Answer 25

Staphylococcus aureus

Question 26

Dominant site of Colonization for S. aureus:

Answer 26

anterior nares axilla perineum 10-15% human skin

Question 27

Principal virulence of S. aureus:

Answer 27

coagulase

Question 28

S. aureus produce_____ or golden – yellow in BAP

Answer 28

lipochrome

Question 29

Beta-hemolytic

Answer 29

S. aureus

Question 30

3 Major Components of Cell Wall Structure of S. aureus.

Answer 30

1. PEPTIDOGLYCAN 2. TEICHOIC ACID 3. PROTEIN A

Question 31

polysaccharide polymer which provide rigid exoskeleton of the cell wall; constitute 40 – 60% of the cell weight

Answer 31

PEPTIDOGLYCAN

Question 32

Elicits production of interleukin-1 and opsonic antibodies by monocytes

Answer 32

PEPTIDOGLYCAN

Question 33

it can be a chemoattractant, have endotoxin-like activity, and activate complement.

Answer 33

PEPTIDOGLYCAN

Question 34

composed of acetylglucosamine and acetylmuramic acid

Answer 34

PEPTIDOGLYCAN

Question 35

polymers of glycerol phosphate (S. epidermidis) or ribitol phosphate (S. aureus)

Answer 35

TEICHOIC ACID

Question 36

plays important role in maintenance of normal physiologic function of the cell

Answer 36

TEICHOIC ACID

Question 37

regulate the cationic environment of cell thus control the activity of autolytic enzyme responsible for growth of cell wall.

Answer 37

TEICHOIC ACID

Question 38

acid antibodies detectable by gel diffusion method

Answer 38

Antiteichoic

Question 39

a bacterial surface protein that has been characterized among a group of adhesins called microbial surface components recognizing adhesive matrix molecules (MSCRAMMS).

Answer 39

PROTEIN A

Question 40

(MSCRAMMS).

Answer 40

microbial surface components recognizing adhesive matrix molecules

Question 41

major protein component of cell wall

Answer 41

PROTEIN A

Question 42

major antigenic determinant unique to Staphylococcus aureus

Answer 42

PROTEIN A

Question 43

antiphagocytic (through binding to the Fc portion of IgG)

Answer 43

PROTEIN A