Determination of the Urine Sugar

Question 1

Normal urine contains sugar _____

Answer 1

Less than 0.1 mg/dl

Question 2

The main sugar detected in the urine is _____

Answer 2

Glucose

Question 3

High concentration of glucose in urine is termed _____

Answer 3

Glucosuria

Question 4

Glucosuria is associated in:

Answer 4

Diabetes mellitus
Impaired tubular reabsorption
Central nervous system damage
Thyroid disorders
Pregnancy with possible latent diabetes mellitus

Question 5

Routine urinary screening for sugars can be done using _____ and _____

Answer 5

Copper Reduction Test
Reagent Strip

Question 6

Tests included in the Copper Reduction Test:

Answer 6

Benedict’s Test
Fehling’s Test
Clinitest

Question 7

Fill in the blank:

Copper reduction tests are based on the ability of glucose and other reducing sugars (lactose, maltose, galactose) to reduce _____ to _____ in the presence of heat and alkali.

Answer 7

Copper sulfate
Cuprous oxide

Question 8

Fill in the blank:

Copper reduction tests are based on the ability of glucose and other reducing sugars (lactose, maltose, galactose) to reduce copper sulfate to cuprous oxide in the presence of _____ and _____.

Answer 8

Heat
Alkali

Question 9

Fill in the blank:

The Copper Reduction Test reaction is shown by a color change progressing from a blue to _____, _____, _____, and _____

Answer 9

Green
Yellow
Orange
Brick Red

Question 10

Fill in the blank:

Reagent strip utilizes the _____

Answer 10

Glucose Oxidase Reaction

Question 11

Fill in the blank:

The test area in the Reagent Strip is impregnated with a mixture of _____, _____, _____, and _____ to produce a double sequential enzyme reaction.

Answer 11

Glucose oxidase
Peroxidase
Chromgen
Buffer

Question 12

Fill in the blank:

The test area in the Reagent Strip is impregnated with a mixture of glucose oxidase, peroxidase, chromogen and buffer to produce a _____.

Answer 12

Double sequential enzyme action

Question 13

Fill in the blanks:

Reagent Strip is based on the catalytic reaction of glucose oxidase between _____ and _____ to produce _____ and _____

Answer 13

Glucose
Oxygen
Gluconic acid
Peroxide

Question 14

Fill in the blanks:

The _____ will catalyze the reaction between peroxide and chromogen to form an oxidized colored compound.

Answer 14

Enzyme peroxidase

Question 15

Fill in the blanks:

The enzyme peroxidase will catalyze the reaction between _____ and _____ to form an oxidized colored compound.

Answer 15

Peroxide
Chromogen

Question 16

Materials needed for the Determination of Urine Sugars using the Copper Reduction Method:

Answer 16

Test tubes
Water bath
Benedict’s reagent
Glucose solution (positive control)
Fehling’s reagent
Clinitest tablets

Question 17

Write down the step-by-step procedure on the Benedict’s Test:

Answer 17

1. Place 2.5 cc of Benedict’s reagent in a test tube.
2. Add 4 drops of urine.
3. Mix Boil for five minutes in a water bath or boil directly over a flame for two minutes.
4. Observe for the reaction and note down the result.

Question 18

In the Benedict’s Test, how many cubic centimeter of Benedict’s reagent is added into the test tube?

Answer 18

2.5 cubic centimeter

Question 19

In the Benedict’s Test, how many drops of urine is added to the slide with the reagent?

Answer 19

4 drops of urine

Question 20

In the Benedict’s Test, if we are to boil the mixture of reagent and urine in the glass slide in a water bath, how many minutes is necessary?

Answer 20

5 minutes

Question 21

In the Benedict’s Test, if the slide with the mixture of urine specimen and reagent are to boil directly over a flame, how many minutes is necessary?

Answer 21

2 minutes

Question 22

Benedict’s Test Grading:

Answer 22

Blue Nil
Green trace
Brown 2+
Yellow-orange 3+
Brick-red 4+

Question 23

Write down the step-by-step procedure in the Fehling’s Test:

Answer 23

1. Mix two cc of Fehling’s A and Fehling’s B solutions.
2. Dilute with an equal volume (4 cc) of distilled water.
3. Heat to boiling and then add urine drop by drop up to one cc using a pipet.
4. Keep the mixture boiling.
5. Observe for the reaction and note down the result.

Question 24

Write down the step-by-step procedure of the Clintest Tablets:

Answer 24

1. Place five drops of urine in a clean test tube.
2. Add 10 drops of water to the urine in the test tube.
3. Place a clinitest tablet in the mixture of urine and water in the test tube.
4. Observe the reaction carefully. If sugar content is excess of 2gm% there is a rapid production of an orange color which quickly turns to brown.
5. Wait 15 seconds after boiling.
6. Shake test tube gently to mix contents.
7. Compare the color produced with clinitest color chart.
8. Record the result.

Question 25

In the Clintest Tablets, how many drops of urine is added in a clean test tube?

Answer 25

5 drops of urine

Question 26

In the Clintest Tablets, how many drops of water is added to the urine in the test tube?

Answer 26

10 drops of water

Question 27

Fill in the blank:

If sugar content is _____ there is a rapid production of an orange color which quickly turns to brown.

Answer 27

Excess of 2gm%

Question 28

Fill in the blank:

If sugar content is excess of 2gm% there is a rapid production of an _____ which quickly turns to _____

Answer 28

Orange color
Brown color

Question 29

In the Clintest Tablets, how much time for us to wait after boiling?

Answer 29

15 seconds

Question 30

Write down the materials needed for the Determination of Urine Glucose using Reagent Strip:

Answer 30

Test tube containing Urine Specimen
Clinistix strip (reagent strip)

Question 31

Write down the step-by-step procedure in the Determination of Urine Glucose using Reagent Strip:

Answer 31

1. Dip the reagent strip in the urine and remove.
2. Stand for one minute and observe for the change in color.
3. Note down the result.