14 Cellular Signaling Flashcards
1
Q
T or F: Cell to cell signaling is also called intracellular communication.
A
False. It should be intercellular communication.
2
Q
What are the four main types of chemical messengers?
A
Endocrine, Paracrine, Autocrine, Neurocrine
3
Q
T or F: Some of the chemical mediators act as more than one type of chemical messengers.
A
True.
4
Q
Give one example of an endocrine messenger.
A
Insulin / Growth hormones
5
Q
Endocrine messengers are also known as ___________.
A
Classical hormones
6
Q
T or F: Paracrine messengers act on distant cells.
A
False. Paracrine messengers act locally to affect nearby, target cells, not distant ones.
7
Q
Through what medium do paracrine messengers diffuse?
A
Interstitial fluid or ECF
8
Q
Which messenger is typically involved in long-distance signaling?
A
Endocrine
9
Q
What is the speed of transport for endcorine messengers: fast or slow?
A
Slow
10
Q
What specialized structure allows direct communication between neighboring cells in juxtacrine signaling?
A
Gap junctions
11
Q
What is another name for juxtacrine messengers?
A
Local hormones
12
Q
T or F: Noradrenaline only functions as a neurotransmitter.
A
False. Noradrenaline functions both as a neurotransmitter and as a classical hormone.
13
Q
What dual roles can dopamine play in the body?
A
Neurotransmitter and a hormone
14
Q
What is another term for autocrine messengers?
A
Intracellular chemical mediators
15
Q
How do endocrine messengers reach their target cells?
A
Bloodstream
16
Q
Leukotrienes are an example of paracrine messengers.
A
False. Leukotrienes are an example of autocrine messengers.
17
Q
What are neurocrine substances also known as?
A
Neural Messengers
18
Q
An endogenous signaling molecule that carries information from one nerve cell to another nerve cell, muscle, or another tissue
A
Neurotransmiter
19
Q
Name two examples of neurotransmitters.
A
- Acetylcholine
- Dopamine
20
Q
Chemical substances released by nerve cells directly into the blood and transported to distant target cells.
A
Neurohormones
21
Q
Give examples of neurohormones
A
- Oxytocin
- ADH
- Hypothalamic releasing hormones
22
Q
What is the primary function of receptors in neurocrine signaling?
A
To sense and respond to changes in signals by activating biochemical pathways within the cell, leading to a defined response.
23
Q
What are chemical mediators and receptors?
A
Protein molecules to which a chemical messenger binds to stimulate a cell response
24
Q
Where are receptors located?
A
On the surface of the cell, within the cytoplasm, or in the nucleus.
25
Neurotransmitters are released at synaptic junctions from nerve cells and act across a narrow synoptic cleft on a post synaptic cell
Neural/Synaptic
26
How do hormones and growth factors reach their target cells in endocrine communication?
Through circulating blood or lymph.
27
What is the difference between paracrine and autocrine communication?
- In paracrine communication, the product of cells diffuses in the extracellular fluid (ECF) to affect neighboring cells
- in autocrine communication, the chemical
messenger binds to receptors on the same cell that secreted it.
28
Name types of chemical messengers that can function as neurotransmitters and paracrine mediators
Amines, amino acids, and steroids
29
What are the specialized junctions that allow intracellular signaling molecules to diffuse from the cytoplasm to one cell to an adjacent cells?
Gap Junctions
30
Where are gap junctions found?
Cardiac and smooth muscles
31
What are the specialized junctions that allows cells to be electrically coupled or permit rapid propagation of electrical activity, which is vitally important for the coordinated activity of cardiac and smooth muscles?
Gap Junctions
32
In neural communication, what are released at the synapse?
Neurotransmitters
33
What kind of communication utilizes neurotransmitters wherein the signal may travel long distances however the effect is limited to a very specific area?
Neural Communication
34
What kind of communication has a relatively slow due to time required for diffusion and the blood flow to reach the target cell?
Endocrine Communication
35
What does endocrine communication utilize to convey the signal?
Hormones
36
What kind of communication involves the secretion of chemicals into the interstitial fluid and primarily affects local areas or nearby cells?
Paracrine Communication
37
9. In Paracrine communication, where are the chemicals secreted?
Interstitial fluid
38
What are specialized junctions allowing intracellular signaling molecules to diffuse from a cell's cytoplasm to its adjacent cell.
Gap junctions
39
Why are gap junctions found in cardiac cells and smooth muscles?
Theey can rapidly propagate electrical activity from cell to cell which is important for coordinated activity of cardiac and smooth muscles
40
Gap junctions can be found in which cells?
Cardiac muscles and smooth muscles
41
T or F: As molecules go from one cell to another through gap junctions, they pass freely through the ECF.
False. They pass freely between cells WITHOUT entering the ECF.
42
Give some examples of substances that can pass via gap junctions.
-Ions
-Sugars
-Amino acid
-Other solutes within required molecular weight
43
In neural communication, at what site are neurotransmitters released?
Synapse
44
T or F:
1) Synaptic signaling is extremely fast or in milliseconds
2) Given its speed, the signal can travel only short distances.
1) True
2) False. It can travel LONG distances.
45
What characteristic of neural communication defines it as limited to a very specific area.
Discrete
46
In endocrine communication, the signaling molecules are released where?
Bloodstream, blood, lymph
47
Aside from signaling molecules, endocrine communication can also release?
Growth factors
48
Give an example of discrete or generalized hormone.
Antidiuretic hormone (ADH) or Vasopressin
49
T or F: The effect of endocrine communication is slow and short.
False. It is slow but prolonged
50
1) What is the type of communication if secreted chemicals affect local or nearby cells?
2) How about if it affects the same cell releasing it?
1) Paracrine communication
2) Autocrine communication
51
What are the 4 steps involved in cell signaling and signal transduction?
1. Recognition
2. Transduction
3. Transmission
4. Response
52
The receptor to which a signaling molecule binds to can be located in?
-Plasma membrane
-Cytoplasm
-Nucleus
53
In recognition, upon binding of a ligand to a receptor, what are activated? Cite some examples.
Intracellular proteins (i.e. kinases, phosphatases, GTP-binding proteins)
54
The signaling proteins interact with? What is their effect on them?
Target proteins; Regulate their activity
55
What are the functions of signaling molecules?
1) Regulate cell growth, division, and differentiation
2) Influence cellular metabolism
3) Modulate intracellular ionic composition
4) Control cytoskeleton-associated events (cell shape, division, migration)
5) Control adhesion (cell-to-cell and cell-to-matrix)
56
How do signaling molecules modulate the ionic composition intracellularly?
Regulating activity of ion channels and transport proteins
57
The recognition of chemical messengers or signal molecules or ligands by cells typically begins by _______
interaction or binding with a receptor at that cell
58
Which serve as cellular switches during recognition?
receptors
59
T or F. Receptors are static components of the cell.
False (receptors are not static components)
60
What do you call the type of regulation wherein there is an increase in the number of receptors when chemical messenger is deficient?
Up-regulation
61
What do you call the type of regulation wherein there is a decrease in the number/activity of receptors when chemical messenger is in excess.
Down-regulation
62
For receptors in the membrane, ______ is responsible for down regulation.
receptor-mediated endocytosis
63
In receptor-mediated endocytosis, when ligands bind to their receptors, what do they form?
ligand-receptor complexes
64
T or F. In receptor-mediated endocytosis, ligand-receptor complexes move internally in the membrane to coated bits
False (Ligand-receptor complexes move LATERALLY in the membrane to coated bits)
65
What do you call the process wherein the coated bits during receptor-mediated endocytosis are subjected to endocytosis to enter the cell?
Internalization
66
Some receptors are either ____ after internalization or ____ by de Novo synthesis in the cell.
recycled; replaced
67
When there is hormone or neurotransmitter in excess, number of active receptors increases or decreases?
decreases
68
When there is deficiency of chemical messengers, the number of active receptors increases or decreases?
increases
69
This is a type of down-regulation, wherein receptors are chemically modified to become less responsive.
Desensitization
70
What are the four major types of receptors according to their intracellular signaling pathways?
Ion Channel-Linked Receptors, G-Protein Coupled Receptors, Enzyme-Linked/Catalutic Receptors, Nuclear Receptors
71
Neurotransmitters directly gating ion channels.
Ionotropic receptors
72
Examples of neurotransmitters.
Acetylcholine and Norepinephrine
73
Examples of this receptor include Nicotinic cholinergic receptor.
Ligand-gated ion channels/Ion Channel-Linked Receptors
74
Examples of this receptor include K+ channel.
Ligand-gated ion channels/Ion Channel-Linked Receptors
75
Examples of this receptor include transient receptor potential cation channel vanilloid member 1 (TRPV1).
Ligand-gated ion channels/Ion Channel-Linked Receptors
76
This receptor mediate direct and rapid signaling between electrically excitable cells.
Ligand-gated ion channels/Ion Channel-Linked Receptors
77
What signaling molecules bind to receptors that cause the opening and closing of ion channels?
Neurotransmitters
78
True or False: Binding of neurotransmitters changes ionic permeability of plasma membrane as well as its membrane potential.
TRUE
79
True or False: The selectivity of Ion Channel-Linked receptor depends on diameter, shape, electrical charges, and chemical bonds along its inside surface.
TRUE
80
[From Book Guyton and Hall Textbook of Medical Physiology, 14th Ed., Ch. 146, Pgs. 574-575] A type of ion channels in the postsynaptic neuronal membrane which usually allow sodium ions to pass when opened (also sometimes allow K+ and/or Ca+).
Cation channel
81
[From Book Guyton and Hall Textbook of Medical Physiology, 14th Ed., Ch. 146, Pgs. 574-575] A type of ion channels in the postsynaptic neuronal membrane which mainly allow chloride ions to pass.
Anion channel
82
[From Book Guyton and Hall Textbook of Medical Physiology, 14th Ed., Ch. 146, Pgs. 574-575] A type of ion channels in the postsynaptic neuronal membrane which are lined with negative charges to attract the positively charged molecules.
Cation channel
83
[From Book Guyton and Hall Textbook of Medical Physiology, 14th Ed., Ch. 146, Pgs. 574-575] A neurotransmitter that opens cation channels (allow positively charges ions) and excite the neuron.
Excitatory transmitter
84
[From Book Guyton and Hall Textbook of Medical Physiology, 14th Ed., Ch. 146, Pgs. 574-575] A neurotransmitter that opens anion channels (allow negatively charges ions) and inhibit the neuron.
Inhibitory transmitter
85
GPCR downstream target proteins that regulate signaling pathways if the target protein is an?
Enzyme
86
GPCR change membrane ion permeability if target protein is an?
ion channel
87
What is the largest and most diverse group of membrane receptors?
G-Protein coupled receptors
88
other names of G-PCR
seven-transmembrane receptor, hepta-helical receptors, seven-helix receptore, serpentine receptors
89
type of receptor that is targeted in drug discovery
G-Protein coupled receptors ( because the cell surface receptors act like an inbox for messages in the form of light energy, peptides, lipids, sugars, and proteins making it diverse)
90
GPCR binds extracellular substances and transmits signals to an intracellular molecule called?
G-Protein (guanine nucleotide binding protein)
91
Name the subunits of G-proteins.
ɑ-subunit , β-subunit, 𝛾-subunit
92
site for attachment of GDP
ɑ-subunit
93
subunit of GPCR that are attached to membrane
ɑ and 𝛾-subunit
94
What are the subunits of a G-protein? (inactive state)
Alpha (ɑ), Beta (β), and Gamma (𝛾)
95
What does the three G-protein subunits form?
Complex
96
What does GDP stand for?
Guanosine diphosphate
97
What does GTP stand for?
Guanosine triphosphate
98
How does the G-protein becomes activated?
Through the binding of a ligand to a GPCR, and once the GDP is released for the binding of GTP
99
Which among the 3 G-protein subunits separates or travels away from the other 2?
The alpha subunit
100
What are the two G-protein subunits that form a signaling molecule together?
The beta and gamma subunits
101
What is the significance of the alpha subunit of a G-protein, especially when activated?
The conversion of GTP to GDP by the alpha subunit.
102
Describe the process of how a GPCR works, including their subunits.
A. The G-protein is inactive, wherein the three subunits form a complex, binding GDP to the alpha subunit. B. To reach an active state, a ligand must bind to the GPCR, followed by the conversion of the GDP to GTP by the alpha subunit. C. The alpha subunit then separates from the combined beta and gamma subunits, which can cause or bring many biological effects. D. The two subunits form together a signaling molecule, which can also activate a variety of effectors. The process repeats the activation and inactivation process, where the alpha subunit converts GTP to GDP and returns to the two subunits.
103
True or False: All receptors when activated function directly as enzymes or are closely associated with enzymes that they activate.
False: Not all, but only some.
104
What are the proteins that pass through the membrane only once in contrast to the seven transmembrane GPCRs?
Enzyme-linked receptors
105
What are the most common enzyme-linked receptors?
Protein kinases or those that are associated with protein kinases.
106
What causes the kinase to phosphorylate a specific subset of proteins on specific amino acids?
Ligand binding
107
True or False: Ligands can only activate protein activity.
False: Ligands can either activate or inhibit the protein activity.
108
True or False: Enzyme-linked receptors are proteins that can pass through the membrane multiple times/
False: Enzyme-linked receptors can only pass through once.
109
What are enzyme-linked receptors composed of?
Hormone-binding sites outside the cell membrane and catalytic/enzyme-binding sites inside
110
True or False: Many enzyme-linked receptors have extrinsic enzyme activity.
False: Many enzyme-linked receptors have intrinsic enzyme activity.
111
Hormone receptors inside the cell where adrenal and gonadal steroid hormones, thyroid hormones, retinoid hormones, and vitamin D bind to rather than in the cell membrane.
Nuclear Receptors
112
Ligands that interact with cytoplasmic receptors.
Glucocorticoids and Mineralocorticoids
113
Ligands that interact with nuclear receptors.
Estrogen, Progesterone, and Thyroid Hormones
114
The activated hormone receptor complex binds to a specific regulatory or promoter sequence of the DNA called __________ which either activates or represses specific chains of mRNA.
Hormone Response Element
115
An __________ can activate a gene response only if the appropriate combination of gene regulatory proteins is present.
Intracellular Receptor
116
The process within cells that converts one type of signal or stimulus into another.
Signal Transduction
117
Signal transduction allows a cell to change its behavior in response to ___________ interactions.
Receptor-Ligand
118
The molecule that initially binds to the receptor, triggering the signal transduction process.
Primary Messengers
119
Intracellular mediators that amplify the response and relay the signal from one part of the cell to another, such as from the plasma membrane to the nucleus, leading to a cascade of cellular events.
Secondary Messengers
120
When acetylcholine binds, these channels increase Na+ permeability, altering the membrane potential and enabling the transmission of electrical signals between excitable cells.
Ion-Channel Activation
121
A ligand binds to an ion channel, causing it to open and allow ions to pass through, relaying signals. This process is crucial in neural synapses for generating action potentials.
Acetylcholine-Gated Na+ Channel
122
What is likely the major site where the anesthetic agents and ethanol exert their effects?
What is likely the major site where the anesthetic agents and ethanol exert their effects?
123
Which ion channel is specifically affected by gamma-aminobutyric acid (GABA) and is an NMDA receptor or N-methyl-D-aspartate receptor?
The GABA-gated Cl- channel
124
How do anesthetic agents and ethanol cause CNS depression?
By altering the functions of ion channels, modifying the electrical activity of neurons through depolarization or hyperpolarization.
125
How do ion channel receptors transduce a chemical signal into a response?
By converting a chemical signal into an electrical signal that elicits a certain response in neurons.
126
What happens when G proteins are bounded to a receptor?
They may either be stimulated or inhibited
127
What do G proteins interact with after being activated?
Different effector proteins or enzymes that catalyze the production of second messengers.
128
What are second messengers, and what is their role?
Second messengers are intracellular signals that affect other intracellular proteins, causing a cascade of events.
129
Besides interacting with effector proteins, what else can G proteins interact with?
G proteins may also interact with nearby ion channels.
130
G-protein will interact with other effector proteins to produce what?
Secondary messengers are intracellular signals that affect other intracellular proteins, causing a cascade of events
131
In its inactive state G-protein (specifically the ɑ- subunit) is bound to what molecule?
GDP (Guanosine Diphosphate)
132
Ligand binding to GPCR will cause a conformational change in the heterotrimeric complex, which will promote the release of what?
GDP (Guanosine Diphosphate) will be released by the ɑ- subunit, and it will be replaced by GTP (Guanosine Triphosphate)
133
When a ligand binds to GPCR, GDP will be replaced by what molecule?
GTP (guanosine triphosphate)
134
What facilitates the dissociation of GDP and binding of GTP in G-proteins
GEFs (Guanine exchange factors)
135
Binding of GTP to G-protein will cause this subunit to dissociate from the rest of the subunits?
ɑ- subunit
136
The β- and ɑ- subunits of G-protein will regulate what?
Downstream effectors (altered activity of enzymes and ion channels)
137
What will inactivate/terminate downstream effectors?
Hydrolysis of GTP bound to ɑ-subunit to GDP
138
What facilitates Hydrolysis of GTP bound to ɑ-subunit
Regulator of G-protein signalling (RGS protein)
139
What is the most common downstream effector which facilitates conversion of ATP to cAMP?
Camp-dependent pathway/adenyl cyclase pathway
140
In the activation of Adenylyl Cyclase, a ligand binds to a receptor that interacts with the alpha G protein composed of:
An alpha subunit of the As class
141
What happens when Adenylyl Cyclase is activated?
Increases cAMP and activated Protein Kinase A
142
In the inhibition of Adenylyl Cyclase, a ligand binds to a receptor that interacts with a G protein composed of:
An alpha subunit of the AI class
143
What happens when Adenylyl Cyclase is inhibited?
Decreases cAMP and decreases Protein Kinase A
144
What does cAMP do?
Activation of Protein Kinase A and Activation of Cyclic Nucleotide-Gated Ion Channels
145
Adenylyl Cyclase Pathway 2nd Messenger:
cAMP
146
What happens when Protein Kinase A is activated?
Catalyze Phosphorylation of Proteins, Phosphorylates CREB (cAMP Response Element -Binding) in nucleus
147
When happens when phosphorylation of proteins is catalyzed?
Stimulation/Inhibition of Enzymes
148
What happens when CREB (cAMP Response Element -Binding) in the nucleus is phosphorylated?
Transcription of genes is regulated
149
Phosphodiester pathway regulates:
Phototransduction
150
When light hits the photoreceptor, the light absorbing retinal portion of the rhodopsin is activated that stimulates the:
Transducin
151
What does Transducin do?
Activates GMP Phosphodiesterase
152
When Transducin activates the GMP Phosphodiesterase, it catalyzes what reaction?
Degradation of cyclic GMP into five GMP
153
Reduction of cyclic GMP causes closure of:
Sodium channels
154
Reduction of cyclic GMP causes closure of the sodium channels which causes:
hyperpolarization of the photoreceptors
155
Phosphodiesterase Pathway 2nd Messenger:
cGMP
156
What are the functions of cGMP as a 2nd messenger in the Phosphodiester Pathway?
cGMP-Regulated Ion Channels and Activates cGMP-Dependent Kinase
157
What does cGMP-Regulated Ion Channels do?
Alters membrane Permeability to Ions
158
When cGMP-Dependent Kinase is activated:
Enzymes are stimulated/inhibited
159
A family of enzymes that modulate a variety of signaling pathways.
Phospholipase
160
An enzyme that converts phosphatidylinositol biphosphate to inositol triphosphate and diacylglycerol.
PLC
161
____diffuses to the endoplasmic reticulum (ER) and activates calcium channels, leading to the release of calcium into the cytosol.
InsP3, Inositol triphosphate
162
____activates protein kinase C, which phosphorylates effector proteins, changing their catalytic activities leading to cellular responses.
DAG, Diacylglycerol
163
An enzyme that releases arachidonic acid from membrane phospholipids.
Phospholipase A2
164
What mediates platelet aggregation, causes airway constriction, and induces inflammation?
Prostaglandins
165
In addition to prostaglandins, what else induces platelet aggregation and constricts blood vessels?
Thromboxanes
166
What inhibits platelet aggregation and dilates blood vessels?
Prostacyclins
167
In a second pathway of arachidonic acid metabolism,_____ initiates the conversion of arachidonic acid
to leukotrienes.
5-lipoxygenase
168
____ participates in allergic and inflammatory responses, including those that cause asthma, rheumatoid arthritis, and inflammatory bowel disease.
Leukotrienes
169
Ligand binding to GPCRs can also activate____.
Phospholipase A2
170
What iniatiates the third pathway of arachidonic acid metabolism?
Epoxygenase
171
T or F: Cells coordinate and integrate external chemical signals, including hormones, neurotransmitters, growth factors, and products of cellular metabolism, that serve as chemical messengers and provide cell-to-cell communication
TRUE
172
T or F: Signals do not interact with receptors located in the plasma membrane, cytoplasm, and nucleus
FALSE
173
An enzyme that facilitates the production of hydroxyeicosatetraenoic acid (HETE) and cis-epoxyeicosatrienoic acid (EETs).
epoxygenase
174
These are classes of receptors that have catalytic activity or associated with proteins that have catalytic activity?
Catalytic Receptor
175
Which class of receptors is associated with Atrial Natriuretic Peptide and Nitric Oxide?
Receptor Guanylyl Cyclase
176
What class of receptor is associated with Transforming Growth Factor-β (TGF-β)?
Receptor Threonine/Serine Kinases
177
Name three signaling molecules that utilize Receptor Tyrosine Kinase.
Epidermal Growth Factor (EGF), Platelet Derived Growth Factor, Insulin
178
Which receptor class is associated with Interleukin signaling?
Tyrosine Kinase Associated Receptors
179
What is the primary action of Receptor Guanylyl Cyclase?
It catalyzes the conversion of GTP to cGMP.
180
What does cyclic GMP activate, and what is the result of this activation?
Cyclic GMP activates cyclic GMP-dependent protein kinase (PKG), which phosphorylates proteins on specific serine and threonine residues.
181
How does Atrial Natriuretic Peptide (ANP) affect the kidney and blood vessels?
ANP inhibits sodium and water reabsorption by the collecting ducts in the kidney, dilates blood vessels, and stimulates Na+ excretion in urine.
182
What effect does nitric oxide (NO) have on smooth muscle?
Nitric oxide (NO) activates soluble guanylyl cyclase, converting GTP to cyclic GMP, which relaxes arteriole smooth muscle.
183
How does nitric oxide relate to the treatment of angina pectoris?
Nitroglycerin boosts NO production, raising cyclic GMP levels, relaxing coronary artery muscles, and treating angina pectoris.
184
What is the primary action of receptor serine/threonine kinases?
Receptor serine/threonine kinases phosphorylate serine or threonine residues in proteins.
185
What triggers the initiation of phosphorylation in receptor serine/threonine kinases?
Phosphorylation is initiated on serine or threonine residues in complementary receptors following ligand binding.
186
What role does TGF-β play in the body?
TGF-β plays a role in embryogenesis and wound healing.
187
What is the consequence of the phosphorylation of the type I subunit in TGF-β receptors?
Activates different downstream substrates and regulatory proteins, leading to the transcription of target genes.
188
What cellular processes are influenced by the activation of TGF-β downstream substrates and regulatory proteins?
Differentiation, chemotaxis, proliferation, and activation of many immune cells.
189
_____ are high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones.
Receptor tyrosine kinases (RTKs)
190
What happens upon ligand binding to nerve growth factor receptors?
Dimerization and activation of tyrosine kinase activity.
191
Describe the composition of insulin receptors and the process of their activation.
Composed of 2 alpha and 2 beta subunits, activation occurs when insulin binds to the alpha subunits, causing a conformational change,
192
What action do activated insulin receptors perform after ligand binding?
Phosphorylates cytoplasmic proteins to initiate its cellular effects.
193
What is the general action of receptor tyrosine kinases?
Phosphorylate tyrosine residues.
194
Describe one of the pathways activated by RTK phosphorylation and its cellular outcome.
One of the pathways involves the activation of the small G-protein Ras,
leading to MAP kinase activation and the production of transcription
factors in the nucleus, which alters gene expression.
195
Which class of catalytic receptors have no intrinsic kinase activity but associate with proteins that have tyrosine kinase activity?
Tyrosine Kinase Associated Receptors
196
Which families of proteins do Tyrosine Kinase Associated Receptors typically associate with?
Tyrosine kinases of SRC family & JAK family
197
What is the “ACTION” of Tyrosine Kinase Associated Receptors?
They interact with cytosolic tyrosine kinases (JAK).
198
Tyrosine Kinase Associated Receptors bind several cytokines including __________-_ and ___________.
Interlukins-6
Erythropoietin
199
Tyrosine Kinase Associated Receptors assemble into homodimers, heterodimers, or heterotrimers during ligand binding. How does this subunit assembly enhance the binding of tyrosine kinases?
It enhances the binding of tyrosine kinases by inducing kinase activity, which then leads to the phosphorylation of tyrosine residues on both the kinases and the receptor.
200
How do Tyrosine Kinase Associated Receptors for cytokine and colony-stimulating factor receptors differ from other growth factor receptors?
They lack tyrosine kinase domains and have little or no cytoplasmic tail but are able to activate Janus tyrosine kinases (JAKs) in the cytoplasm to initiate tyrosine kinase activity.
201
How does the JAK-STAT pathway work?
Tyrosine Kinase Associated Receptors activate Janus Tyrosine Kinases (JAKs) despite lacking tyrosine kinase domains → JAKs then phosphorylate STAT proteins, which form dimers and move to the nucleus to act as transcription factors.
202
How is the family of nuclear receptors categorized?
It is divided into into 2 subfamilies based on structure & mechanism of action.
203
What are the 2 subfamilies of nuclear receptors?
1. Steroid hormone receptors
2. Receptors that bind retinoic acid, thyroid hormones, and vitamin D
204
What happens when ligands (lipid soluble) bind to nuclear receptors?
The ligand-receptor complex activates transcription factors that bind to DNA and regulate the expression of genes.
205
Where can you locate nuclear receptors within the cell?
Cytoplasm
Nucleus
206
Receptors located in the cytoplasm where they interact with chaperones such as heat shock proteins
Cytoplasmic receptors
207
Binding of hormone to cytoplasmic receptors results in:
A conformational change that causes chaperones to dissociate from the receptor; this then uncovers a nuclear location motif that facilitates translocation of the hormone bound receptor complex to the nucleus
208
Examples of cytoplasmic receptors
Glucocorticoid receptor
Mineralocorticoid receptor
209
Nuclear receptors located primarily in the nucleus:
Estrogen receptor
Progesteron receptor
210
Nuclear receptors located in the nucleus, bound to DNA:
Thyroid hormone receptor
Retinoic acid receptor
Vitamin D receptor
211
When activated by hormon binding, nuclear receptors bind to:
Hormone response elements (HREs)
212
What are hormone response elements (HREs)?
They are specific DNA sequences in the regulatory regions of the responsive genes
213
Binding of ligand receptor to DNA causes a conformational change in DNA that:
Initiates transcription
Formation of mRNA
214
How do nuclear receptors regulate gene expression?
By acting as transcriptional repressors
As an example, glucocorticoids suppress the transcription activator protein and nuclear factor which stimulate the expression of genes that cause inflammation. By this, glucocoticoids reduce inflammation
215
(CLINICAL SCENARIO 1) A medical student volunteered in a Medical Mission in North Cotabato, The first patient he saw was a 35- year old man who had continuous, painless diarrhea in the past 24- hours. Stool was described as rice- watery and has a fishy odor.Patient was extremely weak and lethargic, and he complained of muscle cramps. He had sunken eyeballs, his oral mucosa was
dry, and he had poor skin turgor.
- Vital signs:
-Blood Pressure= 60/20 (hypotensive)
-Pulse Rate= 120/min
In this clinical scenario, what do you think happened to the
patient? What do you think is the etiology of the patient’s
symptoms?
The patient may have had an infection with a water- borne
infection called Cholera.
216
What clinical symptoms are associated with cholera?
Cholera presents with severe, painless diarrhea (often described as
“rice water stools”), weakness, lethargy, muscle cramps, sunken
eyeballs, dry oral mucosa, and poor skin turgor.
217
How does cholera toxin affect intestinal epithelial cells?
Cholera toxin is endocytosed by intestinal epithelial cells.
It acts on the G-alpha S subunit, leading to persistent activation of adenylyl cyclase.
This results in overproduction of cyclic AMP (cAMP) and continuous activation of
protein kinase A, leading to chloride loss, sodium loss, electrolyte imbalances,
and water loss.
218
What is the mechanism of action of aspirin?
Aspirin inhibits cyclooxygenase in the arachidonic acid pathway, which has anti-clotting effects.
219
How does Herceptin (used in breast cancer treatment) work?
Herceptin acts on the Receptor Tyrosine Kinase to decrease cancer growth in tumors with HER2 (Human Epidermal Growth Factor Receptor 2) overexpression.
220
The patient in the clinical scenario 1 has a blood pressure of 60/20 mmHg and a pulse rate of 120/min. What is the most likely cause of his hypotension?
Dehydration due to electrolyte loss
221
What is the role of cholera toxin in the pathogenesis of the disease?
It causes continuous activation of adenylyl cyclase, leading to excessive cAMP production.
222
The patient in the clinical scenario 1 has significant electrolyte losses.
Which electrolyte is most likely to be lost in large amounts due to the activation of
CFTR channels by cholera toxin?
Chloride
223
Aspirin inhibits the cyclo-oxygenase enzyme in the arachidonic acid pathway.
What is one clinical effect of this action?
decreased platelet aggregation
224
How does Herceptin help in the treatment of HER2-positive breast cancer?
By blocking the HER2 receptor, reducing cancer cell growth
225
What is the consequence of dysregulation of cellular signaling pathways involving phosphate timers?
Development of certain diseases
226
Which of the following best explains the symptoms of muscle cramps in the patient with cholera?
Electrolyte depletion
227
Given the clinical signs and symptoms, which laboratory test would confirm the diagnosis of cholera?
stool culture
228
Why is it important to monitor platelet function in patients taking aspirin long-term?
Aspirin can cause excessive bleeding by inhibiting platelet aggregation.
