Lesson 10: Culture Methods

Question 1

a population of bacteria grown in the laboratory

Answer 1

culture

Question 2

contains only one single type

Answer 2

pure culture

Question 3

contains two or more different bacteria

Answer 3

mixed culture

Question 4

new media to keep the bacterial population growing

Answer 4

subculture

Question 5

is used to obtain a pure of an infectious agent. also for studies leading to the identification of the pathogen

Answer 5

subculturing

Question 6

means using practices and procedures to prevent contamination from pathogens. it involves applying the strictest rules to minimize the risk of infection

Answer 6

aseptic technique

Question 7

the most effective way to isolate a single type of bacteria from a source that contains many by diluting the individual cells by spreading them over the surface of an agar plate using a platinum or inoculating loop 2-4 mm diameter.

Answer 7

streak plate method

Question 8

also called as carpet culture. this method used for (a) antibiotic susceptibility testing by disk diffusion method, (b) bacteriophage typing, (c) for preparation of bacterial antigens and vaccines

Answer 8

lawn culture

Question 9

provides a pure growth of bacteria for carrying out slide agglutination and other diagnostic tests. it is carried out in tubes usually contained slanted nutrient agar slopes

Answer 9

stroke culture

Question 10

this method is used for (a) mainly for demonstration of gelatin liquefaction (b) demonstration of oxygen requirement of the bacterium under study (c) for the maintenance of stock cultures (d) to study motility of bacteria in semisolid agar.

Answer 10

stab culture

Question 11

piles of bacterial cells observed after an incubation period

Answer 11

colonies

Question 12

used to determine approximate number of viable organism in liquids such as water or urine

Answer 12

pour plate culture

Question 13

a deep culture of agar or gelatin through which the inoculum is evenly distributed by shaking before the medium is solidified.

Answer 13

shake culture

Question 14

this method is used for (a) blood culture and for sterility. (b) dilution in the medium, or (c) large yields culture

Answer 14

liquid cultures

Question 15

incubation of cultures is at? it is the standard practice in the culture of bacteria pathogenic man.

Answer 15

37°C

Question 16

will not grow small inocula unless oxygen is absent and the Eh of the medium is low.

Answer 16

obligate anaerobes

Question 17

a method routinely employed in clinical bacteriology and enables the isolation of distinct colonies

Answer 17

surface plating

Question 18

are widely used for the isolation of pathogens from specimens such as feces, with varied flora

Answer 18

enrichment, selective, and indicator media

Question 19

it have been devised so that, the majority of organisms likely to be associated with those for which the media are used will not grow

Answer 19

selective media

Question 20

favor the multiplication of particular species as a step towards their isolation in pure culture.

Answer 20

enrichment media

Question 21

contain ingredients that change in appearance with particular organisms and so assist their isolation.

Answer 21

indicator media

Question 22

heating at _________ or at higher temperatures for shorter periods.

Answer 22

65°C for 30 minutes