Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

VMCB 311 > CHAPTER 3: Lesson 10: Culture Methods > Flashcards

CHAPTER 3: Lesson 10: Culture Methods Flashcards

1
Q

A population of bacteria grown in the laboratory.

A

culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

This contains only one single type

A

pure culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

It contains two or more different bacteria

A

mixed culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

If a bacterial culture is left in the same media for too long, the cells use up the available nutrients, excrete toxic metabolites, and eventually the entire population will die. Thus bacterial cultures must be periodically ____________, to new media to keep the bacterial population growing

A

subcultured

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

This is used to obtain a pure culture of an
infectious agent, and also for studies leading to the identification of the pathogen.

A

subculturing

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

This means using practices and procedures to prevent contamination from pathogens.

A

Aseptic technique

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

It involves applying the strictest rules to
minimize the risk of infection.

A

Aseptic Technique

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Culture methods

A
  1. Streak plate method
  2. Lawn culture
  3. Stroke Culture
  4. Stab culture
  5. Pour plate culture
  6. Shake culture
  7. Liquid cultures
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

The most effective way to isolate a single type of
bacteria from a source that contains many.

A

Streak plate method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Method used by diluting the individual cells by
spreading them over the surface of an agar plate using a platinum or inoculating
loop of 2–4 mm diameter.

A

Streak Plate Method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Size of a platinum or inoculating
loop used in streak plate method.

A

2–4 mm diameter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

The piles of bacterial cells
observed after an incubation period.

A

colonies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

In the Streak Plate Method, the inoculated culture plate is incubated at _________ overnight
for demonstration of colonies.

A

37°C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

In the Streak Plate Method, this growth occurs at the primary inoculum.

A

confluent growth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Also called as carpet culture

A

Lawn culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

this method is used for Antibiotic susceptibility testing by disk diffusion method

A

Lawn culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

This method is used for Bacteriophage typing.

A

Lawn culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

This is used for preparation of bacterial antigens and vaccines.

A

Lawn Culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

This culture are prepared by flooding the surface of the plate with a liquid culture or suspension of the bacterium, pipetting off the excess
inoculum and incubating the plate

A

Lawn culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

It provides a uniform growth of the
bacterium.

A

lawn culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

It provides a pure growth of bacteria for carrying
out slide agglutination and other diagnostic tests.

A

Stroke culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

It is carried out in tubes usually
containing slanted nutrient agar slopes.

A

Stroke Culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

This method is used for mainly for demonstration of gelatin
liquefaction.

A

Stab culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

This method is used for demonstration of oxygen requirement of the bacterium under
study.

A

Stab Culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

This Method is used for the maintenance of stock cultures.

A

Stab Culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

This method is used to study motility of bacteria in
semisolid agar.

A

Stab Culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

This is used to determine approximate
number of viable organisms in liquids, such as water or urine.

A

Pour plate culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

It is used to
quantitate bacteria in urine cultures and also to estimate the viable bacterial
count in a suspension.

A

Pour Plate Culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

This method is carried out in tubes, each containing 15 mL of
molten agar.

A

Pour Plate Culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

Pour Plate Culture is carried out in tubes, each containing _______ of
________.

A

15 mL
molten agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

The molten agar in tubes in the Pour Plate Culture is left to cool in a water bath at _____.

A

45°C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

In the Pour Plate Culture, after
overnight incubation of these Petri dishes at ______, colonies are found to be
distributed throughout the depth of the medium, which can be counted using
a _________.

A

37°C
colony counter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

A deep culture of agar or gelatin through which the inoculum is
evenly distributed by shaking before the medium is solidified.

A

Shake culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

This method is used
chiefly for the demonstration of anaerobic colonies.

A

Shake Culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

It is made by melting nutrient agar in a test tube.

A

Shake Culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

This method is used for blood culture and for sterility.

A

Liquid cultures

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

This method is used for dilution in the medium,

A

Liquid Culture

38
Q

This method is used for or large yields culture.

A

Liquid Culture

39
Q

It does not provide a pure culture from mixed inocula—the major disadvantage, nor
identify a bacteria.

A

liquid cultures

40
Q

Incubation of cultures at this temperature is standard practice in the
culture of bacteria pathogenic to man.

A

37°C

41
Q

Brucella abortus and capnophilic streptococci, require extra of this element in the air in which they are grown.

A

CO2 or carbon dioxide

42
Q

These organisms grow better in air supplemented with 5 to 10
percent CO2.

A

pneumococcus and gonococcus

43
Q

pneumococcus and gonococcus grow better in air supplemented with how many percent of CO2?

A

5-10%

44
Q

For cultivation of aerobes the incubation is done in an incubator under normal atmospheric condition.

A

Aerobic Culture

45
Q

It require incubation without oxygen and differ in their
requirement and sensitivity to oxygen.

A

Anaerobic bacteria

46
Q

It will not grow from small
inocula unless oxygen is absent and the Eh of the medium is low.

A

Obligate anaerobes

47
Q

METHODS OF ISOLATING PURE CULTURES

A
  1. Surface plating
  2. Use of selective, enrichment or indicator media
  3. Selective treatment of the specimen before culture
  4. Use of selective growth conditions
  5. Separation of motile from non-motile bacteria can be effected using Cragie’s tube
  6. Animal inoculation
  7. Filters
48
Q

a method routinely employed in clinical bacteriology and enables
the isolation of distinct colonies which may be picked out, if necessary for further
purification and study

A

Surface plating

49
Q

widely used for the isolation of
pathogens from specimens such as feces, with varied flora

A

Enrichment, selective and indicator media

50
Q

_________ such as ________ for the diphtheria
bacillus, have been devised so that, the majority of organisms likely to be associated
with those for which the media are used will not grow, and the isolation of pure
cultures is thus facilitated

A

Selective media
tellurite media

51
Q

Selective media such as tellurite media for this kind of bacteria, have been devised so that, the majority of organisms likely to be associated
with those for which the media are used will not grow, and the isolation of pure
cultures is thus facilitated.

A

diphtheria
bacillus

52
Q

This media favor the multiplication of particular species as a step towards their isolation in
pure culture.

A

Enrichment media

53
Q

Example of Enrichment Media

A

selenite broth

54
Q

Enrichment media such as selenite broth is for what type of sp?

A

Salmonella
sp

55
Q

This media contain ingredients that change in appearance with particular
organisms and so assist their isolation.

A

Indicator media

56
Q

Indicator media, such as Willis and Hobbs medium is for what specie?

A

Clostridium sp

57
Q

Example of Indicator Media

A

Willis and Hobbs medium

58
Q

Willis and Hobbs medium is an example of what media?

A

Indicator Media

59
Q

Used to separate spores from vegetative bacilli but does not guarantee that
spores will germinate under subsequent cultural conditions.

A

Heating at 65°C for 30 minutes or at higher temperatures for shorter periods

60
Q

This method is useful for the isolation of tetanus bacilli from dust and
similar source.

A

Selective treatment of the specimen before culture

61
Q

Heating at 65°C for 30 minutes or at higher temperatures for shorter periods.

A

Selective treatment of the specimen before culture

62
Q

This method is the standard
practice for the isolation of tubercle bacilli from sputum and other clinical specimens,
by treatment with alkali, acid or other substances to which most commensals are
susceptible but tubercle bacilli are resistant.

A

Pretreatment of specimens with appropriate bactericidal substances

63
Q

Separation of bacteria with different temperature optima

A

Use of selective growth conditions

64
Q

The temperature
and atmosphere chosen for a culture automatically preclude the growth of many bacteria.

A

Separation of bacteria with different temperature optima

65
Q

Incubation temperature used for most medically important bacteria, is too warm
for some air contaminants, which subsequently appear as colonies when plates are
kept at room temperature.

A

37°C

66
Q

Some pathogens are selectively favoured by growth at what temperature?

A

above 37ºC

67
Q

What bacteria grow at 60ºC?

A

thermophilic bacteria

68
Q

Only thermophilic bacteria grow at what temperature?

A

60ºC

69
Q

Obligate aerobes and
anaerobes may be separated by cultivation under aerobic or anaerobic conditions.

A

Cultivation under aerobic or anaerobic conditions

70
Q

_________ will not grow in air and most __________ grow less
vigorously under anaerobic than under aerobic conditions

A

Strict anaerobes
facultative anaerobes

71
Q

This consists of a tube of semisolid agar, with a narrow tube open at both
ends placed in the center of the medium in such a way that it projects above the level of the agar.

A

Separation of motile from non-motile bacteria can be effected using Cragie’s tube:

72
Q

Separation of motile from non-motile bacteria can be effected using what?

A

Cragie’s tube

73
Q

This also serves the same purpose, inoculation being performed in one
limb and the subculture taken from the other.

A

U-tube

74
Q

This method can also be used to obtain
phase variants in Salmonella species.

A

U-tube

75
Q

U-tube can also be used to obtain
phase variants in what spp?

A

Salmonella spp

76
Q

Pathogenic bacteria may be isolated from mixtures by inoculation into
appropriate animals due to the fact that laboratory animals are highly susceptible to
certain organisms.

A

Animal inoculation

77
Q

example of animal that is highly susceptible to
certain organisms line pneumococcus

A

mouse

78
Q

Mouse is highly susceptible to
certain organisms like what?

A

Pneumococcus

79
Q

Animal inoculation

A
  1. Pnemococcus
  2. Anthrax bacilli
  3. Tubercle bacillus
80
Q

If a mixture of organisms containing the pneumococcus, e.g. in sputum, is inoculated subcutaneously into a mouse, the animal dies because of what?

A

pneumococcal septicemia

81
Q

If a mixture of organisms containing the pneumococcus, e.g.
in sputum, is inoculated subcutaneously into a mouse, the animal dies of
pneumococcal septicemia in how many hours?

A

12 to 48 hrs

82
Q

Pnemococcus can be obtained in
pure culture from the ________.

A

heart blood

83
Q

It can be distinguished from other aerobic
sporulating bacilli by inoculation into mice or guinea pigs.

A

Anthrax bacilli

84
Q

It produces a
fatal septicemia and may be cultured pure from the heart blood.

A

Anthrax bacilli

85
Q

Anthrax bacilli can be distinguished from other aerobic
sporulating bacilli by inoculation into what animals?

A

mice or guinea pigs

86
Q

Anthrax bacilli produce a
_________.

A

fatal septicemia

87
Q

Anthrax bacilli produce a
fatal septicemia and may be cultured pure from where?

A

heart blood

88
Q

This can be isolated from
contaminating organisms by inoculation of an infected specimen into a guinea pig.
.

A

Tubercle bacillus:

89
Q

Tubercle bacillus can be isolated from contaminating organisms by inoculation of an infected specimen into a what animal?

A

guinea pig

90
Q

Bacteria of differing sizes may be separated by the use of these.

A

Filters

91
Q

These are widely used for separating viruses from bacteria.

A

Filters

VMCB 311 (11 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions