CHAPTER 3: Lesson 11: ASEPTIC TECHNIQUE

Question 1

What is the main objective of aseptic technique?

Answer 1

to eliminate contaminants from
work surfaces, solutions and other sources in the laboratory

Question 2

Contamination can be avoided by the ff:

Answer 2

(1) regular checking of culture
(2)
avoidance of use of antibiotics for at least a part of incubation period to reveal cryptic
contaminations
(3) always use sterile reagents and materials, (4) exclusive use of
media, materials for different cell lines
(5) strict observation of standard sterile technique at all times.

Question 3

Elements of aseptic environment

Answer 3

1. Quiet area
2. Work surface
3. Personal Hygiene
4. Reagents and media
5. Cultures

Question 4

In the absence of a laminar flow or sterile room, a this area with little
or no traffic and no other activity should be picked.

Answer 4

Quiet Area

Question 5

The area should be kept clean
and not contain equipment other than that related to culture.

Answer 5

Quiet Area

Question 6

Non sterile activities
should not be carried out in this area.

Answer 6

Quiet Area

Question 7

Maintain clean and tidy ________ by (a) completely clear
surface, (b) swab surface with 70% alcohol before, between and after procedures, (c)
place onto the work surface materials necessary for a certain procedure, (d) arrange
work area systemically for easy access of items used and wide clear space to work
on, (e) work within range of vision, (f) mop up any spillage immediately and swab
area with 70% alcohol, and (g) when finished, remove all materials from the work
surface and swab with 70% alcohol.

Answer 7

Work surface

Question 8

hand washing is encouraged to reduce bacterial
count/adherent microorganisms on the skin that would otherwise likely to blow onto
your culture and cause contamination.

Answer 8

Personal Hygiene

Question 9

Surgical gloves may be worn and swabbed
frequently, and disposed properly after procedure.

Answer 9

Personal hygiene

Question 10

Long hair are tied back or kept in a cap, avoid talking when doing a procedure if not working in a laminar
flow hood, or avoid doing any culture procedures when having any respiratory
symptoms.

Answer 10

Personal hygiene

Question 11

This is encouraged to reduce bacterial
count/adherent microorganisms on the skin that would otherwise likely to blow onto
your culture and cause contamination.

Answer 11

hand washing

Question 12

These are ensured to be sterile
within packages.

Answer 12

Reagents and media

Question 13

Be cautious when handling these from other laboratories as they
may be contaminated from the source or during transit.

Answer 13

Cultures

Question 14

Newly arrived should be handled separately from the rest of the stocks and avoid antibiotics until
they are shown to be uncontaminated.

Answer 14

Cultures

Question 15

Sterile Handling

Answer 15

1. Swabbing
2. Capping
3. Flaming
4. Handling Bottles and Flasks
5. Pipetting
6. Pouring

Question 16

swab surface with 70% alcohol before, between and after procedures.
Swab other materials if applicable before use.

Answer 16

Swabbing

Question 17

Swab surface with this before, between and after procedures.

Answer 17

70% alcohol

Question 18

Screw caps should be washed thoroughly and covered with aluminum foil
to protect neck of bottle from dust.

Answer 18

Capping

Question 19

Type of steril handling wherein the neck of bottles and screw caps or other materials before and
after opening, before and after closing it to avoid contamination.

Answer 19

Flaming

Question 20

when working on an open bench, these should not be kept vertical when opened and kept at an angle as shallow as
possible during manipulations to avoid contamination

Answer 20

Handling Bottles and Flasks

Question 21

the easiest way to
manipulate liquids

Answer 21

Pipetting

Question 22

It should be avoided to avoid mycoplasmal
contamination and prevent hazard to the operator.

Answer 22

Mouth pipetting

Question 23

It should be avoided as much as possible to prevent generation of a bridge of
liquid between the outside of the bottle and the inside, which may permit contamination to enter the bottle.

Answer 23

Pouring

Question 24

An equipment that protect work surface from dust and contamination by a
constant, stable flow of filtered air passing over the work surface.

Answer 24

Safety cabinets (Laminar Flow)

Question 25

Two types of Safety cabinets (Laminar Flow)

Answer 25

• horizontal
• vertical

Question 26

Where air blows from the side facing you, parallel to the work surface, and
not circulated;

Answer 26

horizontal

Question 27

Where air blows down from the top of the hood onto the
work surface and is drawn through the work surface and either recirculated or vented.

Answer 27

vertical

Question 28

These are used to sterilize the air and exposed work surfaces in laminar flow hoods between uses.

Answer 28

Ultraviolet lights

Question 29

These are a major source of contamination.

Answer 29

Humidified incubators

Question 30

Incubators should
be regularly cleaned with detergent and then swabbed with ________ and cleared
of any inappropriate contents.

Answer 30

70% alcohol

Question 31

The humidifier tray at the bottom of the incubator can
be supplemented with a _______ to retard fungal growth.

Answer 31

fungicide