Enzymes

Question 1

Aim

Answer 1

Determine the time taken for starch to be digested and to calculate the rate of enzyme activity at different pH levels

Question 2

Step one

Answer 2

Add 2 cm³ of each solution ranging from pH 3 to 7 into each labelled test tube using a syringe

Question 3

Step two

Answer 3

Immerse test tube, starch and Amylase solution in water bath at 25°C

Question 4

Step three

Answer 4

Place 2 cm³ of Aminé solution into test tube of buffer solution

Question 5

Step four

Answer 5

Place 2 cm³ of starch into test tube and start time

Question 6

Step five

Answer 6

Repeat step for every 30 seconds until the iodine solution remains brown and does not turn blue black

Question 7

Step six

Answer 7

Calculate the rate of enzyme action using:
1 / Time taken for iodine solution to remain brown

Question 8

Step seven

Answer 8

Repeat steps 1–6 for different Ph values