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Biology 241 Lab EXAM > Lab 2: Enzymes and Spectrophotometry > Flashcards

Lab 2: Enzymes and Spectrophotometry Flashcards

1
Q

Enzymes

A

Biological catalysts that increase rates of biochemical reactions

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2
Q

Activation Energy

A

Energy required for a reaction to occur

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3
Q

Substrate

A

Reactant that binds to the active site of an enzyme

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4
Q

Product

A

Molecule formed by the conversion of a substrate by an enzyme

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5
Q

Enzyme-Substrate Complex

A

Binding of enzyme and substrate to facilitate a chemical change

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6
Q

Enzyme Kinetics

A

Study of how fast an enzyme converts substrate to products

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7
Q

Enzyme Activity

A

Rate or velocity of a reaction catalyzed by an enzyme

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8
Q

Spectrophotometry

A

Measurement of light absorption by a substance

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9
Q

Standard Curve

A

Graph showing the relationship between absorbance and concentration

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10
Q

Absorbance

A

Amount of light absorbed by a substance

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11
Q

Concentration

A

Amount of a substance in a given volume

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12
Q

β-Galactosidase

A

Enzyme that breaks down lactose into glucose and galactose

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13
Q

Lactose Intolerance

A

Inability to digest lactose due to low levels of β-galactosidase

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14
Q

ONPG

A

Artificial substrate for β-galactosidase activity measurement

ortho-Nitrophenyl-β-galactoside

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15
Q

o-Nitrophenol

A

Pale yellow product formed by the hydrolysis of ONPG

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16
Q

o-Nitrophenolate

A

Dark yellow product formed by the conversion of o-nitrophenol using Alkine solution such as Sodium carbonate

17
Q

Dilutions

A

Mixing a volume of a stock solution with a volume of diluent

18
Q

Dilution Factor

A

Total volume of a solution divided by the volume of the original solution and the volume of dilutent

(volume of solution) / (volume of solution + dilutent)

19
Q

Fixed Volumes

A

Specific volumes of known concentration prepared from stock solutions

V1C1 = V2C2

Biology 241 Lab EXAM (7 decks)

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