Lab 6: Photosynthesis and the Hill Reaction

Question 1

Photosynthesis

Answer 1

Process by which plants convert light energy and CO2 into organic molecules

Question 2

Hill reaction

Answer 2

Reduction of an electron acceptor by electrons and protons from water, producing oxygen

Question 3

Spectrophotometer

Answer 3

Instrument used to measure the absorbance of light by a sample

Question 4

Chloroplasts

Answer 4

Organelles in plants responsible for photosynthesis

Question 5

Light-dependent reactions

Answer 5

Reactions in photosynthesis that convert sunlight energy to ATP and NADPH

Question 6

Light-independent reactions

Answer 6

Reactions in photosynthesis that use ATP and NADPH to convert CO2 to carbohydrate

Question 7

Electron transport chain (ETC)

Answer 7

Series of protein complexes that transfer electrons and generate ATP

Question 8

Chemiosmosis

Answer 8

Process by which ATP is synthesized using an electrochemical gradient

Question 9

Calvin Cycle

Answer 9

Process in photosynthesis where CO2 is reduced to carbohydrate

Question 10

Antennae complexes

Answer 10

Photosynthetic pigments that absorb light energy and transfer it to reaction centers

Question 11

Photosystems I and II

Answer 11

Reaction centers in chloroplasts that convert light energy to chemical energy

Question 12

Proton Motive Force

Answer 12

Electrochemical gradient across the thylakoid membrane that powers ATP synthesis

Question 13

Photolysis

Answer 13

Splitting of water into protons and oxygen in the presence of light

Question 14

Robert Hill

Answer 14

Scientist who discovered the Hill reaction and the source of oxygen in photosynthesis

Question 15

Chlorotoluron

Answer 15

Chemical inhibitor of photosynthesis used in Experiment II

Question 16

Inhibitor

Answer 16

Substance that reduces or prevents a biological process

Question 17

Null hypothesis

Answer 17

Statement that there is no significant difference or relationship between variables

Question 18

Alternate hypothesis

Answer 18

Statement that there is a significant difference or relationship between variables

Question 19

Independent variable

Answer 19

Variable manipulated by the experimenter

Question 20

Dependent variable

Answer 20

Variable that is measured or observed in response to the independent variable

Question 21

Control

Answer 21

Standard for comparison in an experiment, used to establish a baseline

Question 22

Buffer

Answer 22

Solution that resists changes in pH

Question 23

DCPIP

Answer 23

Artificial electron acceptor used to measure the Hill reaction

Question 24

Absorbance

Answer 24

Measure of the amount of light absorbed by a sample

Question 25

Stock solution

Answer 25

Concentrated solution used to prepare dilutions

Question 26

Dilution

Answer 26

Process of reducing the concentration of a solution by adding solvent