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microbiology > Lab > Flashcards

Lab Flashcards

1
Q

5 nutritional needs of MOs

A

Water
Nitrogen
Carbon
Minerals
Buffer

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2
Q

Best pH for fungi?

A

5-6

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3
Q

List 5 aerobic MOs families

A

Pseudomonas
Brucella
Neisseria
Bordetella
Fracisella

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4
Q

What is enrichment incubation?

A

Selective incubation for a specific spp

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5
Q

Minimum bacteria/mL for turbidity in a broth?

A

10^6

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6
Q

Agarose temperature landmarks

A

<95 for sterilization
55-60 for distribution
<50 for incubation

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7
Q

Agar deep vs slant

A

Deep = Anaerobic
Slant= aerobic

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8
Q

4 agars that are selective + differential ?

A

MacConkey
Mannitol salt
Hektoen enteric
XLD

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9
Q

What is mannitol salt agar selective for and how?

A

Staphylococcus > salty

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10
Q

What bacteria is non lactose fermenter G-? What is its color on Hektoen enteric agar?

A

Shigella ; blue

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11
Q

What bacteria is lactose fermenter G-? What is its color on Hektoen enteric agar?

A

E. Coli ; yellow

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12
Q

What bacteria is H2S producer G-? What is its color on Hektoen enteric agar?

A

Salmonella ; black

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13
Q

XLD vs Hektoen Enteric?

A

Shigella>
Blue in HE ; Pink in XLD

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14
Q

What MO has a swarming colony?

A

Proteus

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15
Q

What is thayer martin selective forv

A

N meningitis & N gonorrhea

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16
Q

Which bacteria gram stain are resistant to vancomycin?

A

G-

17
Q

Which bacteria gram stain are resistant to colistin & polymyxin?

A

G+

microbiology (16 decks)

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