Autoclave

Question 1

3 parts of autoclave

Answer 1

1,chamber
2,lid
3,heating heater

Question 2

Conditions for sterilisation

Answer 2

121degree c
15lb per sq inch pressure
15 min holding time

Question 3

Uses

Answer 3

*Surgical ,anaesthetic, gloves,rubber materials
*materials which withstand at low temp
*biomedical waste

Question 4

Biological indicator of sterilisation

Answer 4

Spores of geobacillus stearothermophilus

Question 5

Holding period is counted when

Answer 5

On opening the safety valves, the steam escapes

Question 6

Autoclave

Answer 6

-Moist temp above 100oC
-load to saturated steam
-pressure Greater than atmospheric pressure

Question 7

Principle of autoclave

Answer 7

Vapour pressure of h20 =surrounding atmosphere
-pressure inside the vessel^,temp of h20^
-steam to the cooler surface-condense to h20-gives up latent heat to that surface

Question 8

Components of autoclave

Answer 8

-chamber
-steam jacket
-lid
-discharge tap consists of pressure gauge safety valve

Vertical /horizontal gunmetal or stainless steel in iron case
Lid closed by screw clumps and washer makes it air tighter
On lid ,a discharge tap for steam and air

Question 9

Working of autoclave(for sterilization)

Answer 9

-sufficient h20 in cylinder
-material put in the tray and autoclave is heated
-in lid with opening of discharge tap screw it tight
-safety valve to required pressure
-until all the air is displaced, mixed steam air is allowed to escape (to test, pass steam air through pale water)
-if no more air bubble, close the discharge tap
-safety valve opens, when steam inside reaches desired level
-excess steam escape
-holding temp and temp calculated
-heater turned off ,allow autoclave to cool
When pressure inside=atmospheric pressure

Question 10

Precautions of autoclave

Answer 10

-not used to sterilize waterproof materials (oil,grease,glove powder)
-materials loaded Ina way thatefficient steam must be penetrated
-materials should not touch the top and side of chamber
-

Question 11

Typed of autoclave

Answer 11

-simple laboratory
-transportable bench top
-large simple-horizontal and door at one side
-downward displacement
-multipurpose laboratory -to sterilize heat labile media ,glassware ,other equipments,lab coat contaminated discarded materials
-High security -to sterilize high risk organism
-porous load -hospital service, surgical theatre