ELECTROPHORESIS OF NUCLEIC ACIDS Flashcards
1
Q
elements of electrophoresis
A
-gel
-buffer
-dyes
-intercalating molecules
2
Q
gel for big molecules (between 100 and 50.000 NTP)
A
polyacrylamide
3
Q
gel for small molecules
A
agarose
4
Q
buffer
A
electric conduction
5
Q
dyes
A
label molecules with bromophenol blue to see the migration
6
Q
intercalting molecules
A
detection
7
Q
to study JUST the size difference
A
double stand non denatured DNA
8
Q
to study size + conformation
A
denatured single stranded DNA and RNA
9
Q
A
