molecular hybridization Flashcards
1
Q
dehybridizaion
A
denaturation
2
Q
denaturation is possible when… (5)
A
- increasing temperature
- changing the medium composition (decrease of Na, increase of pH, adding denaturing molecules)
3
Q
melting temperature
A
4(C+G) + 2(A+T)
4
Q
stringency is…
A
degree of inhibition of non specific hybridization
5
Q
high stringency
A
only homologous sequences will be paired
6
Q
procedures comprising a hybridization (3)
A
- in solution : PCR primers
- on membrane : southern/northen/dot blot
- on tissue : in situ hybridization
7
Q
A
