Ch 29 molecular diagnostics Flashcards

1
Q

What is targeted for Equine influenza virus isolation by PCR?

A

hemagglutinin
nucleoprotein
matrix gene
**are commonly targeted genes

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2
Q

What is the sample of choice for testing for EHV-1 and EHV-4?

A

nasal or nasopharyngeal swab virus isolation/PCR

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3
Q

Streptococcus equi subsp equi culture may be unsuccessful for what reasons?

A

–during incubation & early clinical phases of infection
-presence of other Beta-hemolytic streptococci especially S. equi subsp zooepidemicus

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4
Q

What is one of the pitfalls in using PCR as a diagnosis?

A

inability to distinguish between live and nonviable organisms

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5
Q

PCR for Strep equi subsp equi should be considered in what scenerios?

A

-detect asymptomatic carriers
-establish the S. equi infection status of asymptomatic horses
-determine the success of elimination of S. equi from the guttural pouch

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6
Q

What is considered the gold standard diagnosis of Rhodococcus equi?

A

culture of the organism from TTW fluid

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7
Q

PCR for what gene of R equi in a TW fluid sample from a foal with pneumonia can be considered diagnostic?

A

Vap A gene

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8
Q

PCR for testing of neural tissue is useful when?

A

for post mortem testing

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9
Q

WNV encephalitis diagnostics

A

-one or more of the following– isolation of WNV from blood, CSF or tissue
-four fold increase in plaque reduction neutralization antibody titers on paired serum samples taken two weeks apart
-detection of IgM antibody to WNV by IgM capture ELISA

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10
Q

Which serotype of EHV-1 is associated with neurologic outbreaks?

A

D752 genotype

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