Reading 2 Flashcards

1
Q

Dispersive replication

A

both nucleotide strands break down into fragments, always produces hybrids b/c has parts of original and new

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2
Q

how many stages does replication have

A

4

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3
Q

the stages of replication

A

initiation, unwinding, elongation termination
(I understand every thing)

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4
Q

-ase

A

enzyme

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5
Q

helicase

A

unzipping enzyme: unzips the 2 strands in DNA and breaks the hydrogen bonds

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6
Q

primase

A

the initializer, makes primer so the DNA polymerase can find where to start working

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7
Q

primer is made of what

A

RNA

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8
Q

ligase

A

glues the DNA fragments together

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9
Q

DNA replication starts where

A

the origin

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10
Q

step 1 of replication

A

helicase comes to unzip the DNA strands

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11
Q

step 2 (1 of 2) of replication

A

-single-stranded binding proteins bind to the strands of DNA to keep them separated

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12
Q

step 2 (2 of 2) of replication

A

topoisomerase keeps the DNA from supercoiling

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13
Q

step 3 of replication

A

primase makes primers on both strands

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14
Q

step 4 of replication

A

DNA polymerase builds the new strands
-has a leading strand and a lagging strand

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15
Q

lagging strand

A

(on 5 to 3)
-lagging because primers have to keep being placed and are forming fragments called Okazaki fragments

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16
Q

Okazaki fragments

A

formed in the lagging strand of the DNA

17
Q

step 5 of replication

A

ligase comes in the fill the gaps between the Okazaki fragments

18
Q

step 6 of replication

A

DNA polymerase proofreads its work for mistakes

19
Q

result of replication

A

2 DNA strands

20
Q

semi conservative

A

each resulting DNA molecule has 1 old original strand