OSPE Neisseria meningitidis- bacterial meningitis

Question 1

specimen

Answer 1

CSF
Blood culture

Question 2

Case

Answer 2

-splenectomised- more susceptible to infection by capsulated organism
-high grade fever, headache and neck rigidity.
-CSF was sent for Gram stain and culture.

Question 3

Gram-stain

Answer 3

• pus cells seen
• Both intracellular and extracellular GN diplococci seen
• Presumptive diagnosis: Meningococcal meningitis

Question 4

culture

Answer 4

• Blood agar:Non-hemolytic colonies
• Chocolate agar:Better growth, larger colonies
• MacConkey agar: No growth

Question 5

Biochemical identification

Answer 5

1. Catalase test: Positive
2. Oxidase test: Positive
3. Sugar fermentation test:
   ▪ Glucose - Fermented
   ▪ Maltose - Fermented
   ▪ Sucrose – Not fermented

*N. gonorrheae ferments only glucose

Question 6

Serogrouping

Answer 6

Latex agglutination kit